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  4. Recombinant Mouse Serine/threonine-protein kinase Nek8 (Nek8)

Recombinant Mouse Serine/threonine-protein kinase Nek8 (Nek8)

  • 中文名称：
    小鼠Nek8重组蛋白
  • 货号：
    CSB-YP849695MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Nek8重组蛋白
  • 货号：
    CSB-EP849695MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Nek8重组蛋白
  • 货号：
    CSB-EP849695MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Nek8重组蛋白
  • 货号：
    CSB-BP849695MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Nek8重组蛋白
  • 货号：
    CSB-MP849695MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Nek8
  • Uniprot No.：
  • 别名：
    Nek8; Jck; Serine/threonine-protein kinase Nek8; EC 2.7.11.1; Never in mitosis A-related kinase 8; NimA-related protein kinase 8
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-698
  • 氨基酸序列
    MEKYERIRVV GRGAFGIVHL CLRKADQKLV ILKQIPVEQM TKEERQAAQN ECQVLKLLNH PNVIEYYENF LEDKALMIAM EYAPGGTLAE FIQKRCNSLL EEETILHFFV QILLALHHVH THLILHRDLK TQNILLDKHR MVVKIGDFGI SKILSSKSKA YTVVGTPCYI SPELCEGKPY NQKSDIWALG CVLYELASLK RAFEAANLPA LVLKIMSGTF APISDRYSPE LRQLVLSLLS LEPAQRPPLS HIMAQPLCIR ALLNIHTDVG SVRMRRAEKS LTPGPPIASG STGSRATSAR CRGVPRGPVR PAIPPPLSSV YAWGGGLSSP LRLPMLNTEV VQVAAGRTQK AGVTRSGRLI LWEAPPLGAG GGTLLPGAVE LPQPQFVSRF LEGQSGVTIK HVACGDLFTA CLTDRGIIMT FGSGSNGCLG HGNLTDISQP TIVEALLGYE MVQVACGASH VLALSTDGEL FAWGRGDGGR LGLGTRESHN CPQQVPVAPG QEAQRVVCGI DSSMILTSPG RVLACGSNRF NKLGLDHLSL DEEPVPYQQV EEALSFTPLG SAPLDQEPLL CVDLGTAHSA AITASGDCYT FGSNQHGQLG TSSRRVSRAP CRVQGLEGIK MVMVACGDAF TVAVGAEGEV YSWGKGTRGR LGRRDEDAGL PRPVQLDETH PYMVTSVSCC HGNTLLAVRS VTDEPVPP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for renal tubular integrity. May regulate local cytoskeletal structure in kidney tubule epithelial cells. May regulate ciliary biogenesis through targeting of proteins to the cilia. Plays a role in organogenesis and is involved in the regulation of the Hippo signaling pathway.
  • 基因功能参考文献：
    1. NEK8 plays a critical role in replication fork stability through its regulation of the DNA repair and replication fork protection protein RAD51. PMID: 27892797
    2. NEK8-depleted inner medullary collecting duct cells exhibited a defective response to fluid shear. PMID: 23274954
    3. Nek8 acts downstream of Inv function PMID: 22687244
    4. cross-species analysis between zebrafish and mouse demonstrates taht NEK8 has a role in development of pronephric cysts PMID: 12421721
    5. Nek8 and Pkd1 genes function in a common pathway to regulate cystogenesis. Moreover, compound Pkd1 and Nek8 heterozygous adult mice develop a more aggressive cystic disease than animals with a mutation in either gene alone. PMID: 17928412
    6. Defects in ciliary localization of Nek8 is associated with polycystic kidney diseases PMID: 18189147
    7. interacts with the signal transduction pathways of the polycystins and may lead to cystogenesis by altering the structure and function of cilia in the distal nephron PMID: 18235101

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  • 相关疾病：
    Defects in Nek8 are the cause of autosomal recessive juvenile polycystic kidney disease (ARJPKD).
  • 亚细胞定位：
    Cytoplasm. Cytoplasm, cytoskeleton. Cell projection, cilium. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
  • 蛋白家族：
    Protein kinase superfamily, NEK Ser/Thr protein kinase family, NIMA subfamily
  • 组织特异性：
    Kidney, liver, and testis.
  • 数据库链接：

    KEGG: mmu:140859

    STRING: 10090.ENSMUSP00000017549

    UniGene: Mm.23788