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  4. Recombinant Mouse Serine/threonine-protein kinase DCLK1 (Dclk1)

Recombinant Mouse Serine/threonine-protein kinase DCLK1 (Dclk1)

  • 中文名称：
    Recombinant Mouse Serine/threonine-protein kinase DCLK1(Dclk1),Yeast
  • 货号：
    CSB-YP864369MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse Serine/threonine-protein kinase DCLK1(Dclk1),Yeast
  • 货号：
    CSB-EP864369MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse Serine/threonine-protein kinase DCLK1(Dclk1),Yeast
  • 货号：
    CSB-EP864369MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse Serine/threonine-protein kinase DCLK1(Dclk1),Yeast
  • 货号：
    CSB-BP864369MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse Serine/threonine-protein kinase DCLK1(Dclk1),Yeast
  • 货号：
    CSB-MP864369MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Dclk1; Dcamkl1; DclkSerine/threonine-protein kinase DCLK1; EC 2.7.11.1; Doublecortin-like and CAM kinase-like 1; Doublecortin-like kinase 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-756
  • 氨基酸序列
    MSFGRDMELE HFDERDKAQR YSRGSRVNGL PSPTHSAHCS FYRTRTLQTL SSEKKAKKVR FYRNGDRYFK GIVYAISPDR FRSFEALLAD LTRTLSDNVN LPQGVRTIYT IDGLKKISSL DQLVEGESYV CGSIEPFKKL EYTKNVNPNW SVNVKTTSAS RAVSSLATAK GGPSEVRENK DFIRPKLVTI IRSGVKPRKA VRILLNKKTA HSFEQVLTDI TDAIKLDSGV VKRLYTLDGK QVMCLQDFFG DDDIFIACGP EKFRYQDDFL LDESECRVVK STSYTKIASA SRRGTTKSPG PSRRSKSPAS TSSVNGTPGS QLSTPRSGKS PSPSPTSPGS LRKQRISQHG GSSTSLSSTK VCSSMDENDG PGEGDELGRR HSLQRGWRRE ESEEGFQIPA TITERYKVGR TIGDGNFAVV KECIERSTAR EYALKIIKKS KCRGKEHMIQ NEVSILRRVK HPNIVLLIEE MDVPTELYLV MELVKGGDLF DAITSTSKYT ERDASGMLYN LASAIKYLHS LNIVHRDIKP ENLLVYEHQD GSKSLKLGDF GLATIVDGPL YTVCGTPTYV APEIIAETGY GLKVDIWAAG VITYILLCGF PPFRGSGDDQ EVLFDQILMG QVDFPSPYWD NVSDSAKELI NMMLLVNVDQ RFSAVQVLEH PWVNDDGLPE NEHQLSVAGK IKKHFNTGPK PSSTAAGVSV IATTALDKER QVFRRRRNQD VRSRYKAQPA PPELNSESED YSPSSSETVR SPNSPF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system.
  • 基因功能参考文献：
    1. Dclk1 contributes functionally to the pathogenesis of pancreatic cancer; Dclk1 marks quiescent pancreatic progenitors that are candidates for the origin of pancreatic cancer PMID: 27058937
    2. loss of parietal cells leads to the reversible emergence of a novel Dclk1-expressing sensory cell population in the gastric mucosa. PMID: 26073039
    3. Dclk1 plays an important role in regulating colonic inflammatory response and colonic epithelial integrity PMID: 26285154
    4. Dclk1 promotes axonal regeneration, neuronal survival, and growth core reformation via microtubule stabilization, prevention of F-actin destabilization and synergy with mTOR. PMID: 26526391
    5. Dclk1 is critically involved in facilitating intestinal tumorigenesis by enhancing pluripotency and EMT factors in Apc mutant intestinal tumors and it also provides a potential therapeutic target for the treatment of colorectal cancer. PMID: 25211188
    6. Dclk1 plays a functional role critical in the epithelial restorative response. PMID: 24123696
    7. DCLK1 regulates pluripotency and angiogenic factors via microRNA-dependent mechanisms in pancreatic cancer. PMID: 24040120
    8. These results suggest that crypt epithelial cell Dclk1 expression can be used as one potential marker to evaluate the early survival of intestinal stem cells following severe radiation injury. PMID: 24368703
    9. Pancreatic neoplasms in mice contain morphologically and functionally distinct subpopulations, expressing DCLK1, that have cancer stem cell-like properties. PMID: 24096005
    10. Doublecortin-like kinases are critical regulators of dendritic development by means of their specific targeting to the distal dendrites, and their local control of dendritic growth and synapse maturation. PMID: 23385585
    11. The neurons lacking Dcx and/or its structurally conserved paralogue, doublecortin-like kinase 1 (Dclk1), show impaired Kif1a-mediated transport of Vamp2, a cargo of Kif1a, with decreased run length. PMID: 22857951
    12. Our findings define DCL as a novel marker for specific aspects of adult neurogenesis, which partly overlap with doublecortin PMID: 22605579
    13. Gastric tuft cells express DCLK1 and are expanded in hyperplasia PMID: 21688022
    14. A truncated, constitutively active form of DCLK-short is highly expressed in the hippocampus, cortex and amygdala of transgenic mice. These mice spend less time, move less in the open arms of the maze and show a reduction in the number of rim dips. PMID: 20705078
    15. The DCLK gene product affects glutamatergic neuronal transmission in response to neurological stimuli. PMID: 20659433
    16. genomic organization underlying the splice variants of DCLK and the expression profile of two splice variants affecting the kinase domain of DCLK and CPG16 (candidate plasticity gene 16). PMID: 11884394
    17. DCLK controls mitotic division by regulating spindle formation and also determines the fate of neural progenitors during cortical neurogenesis. PMID: 16387637
    18. Dcx and Dclk may directly or indirectly regulate microtubule-based vesicle transport, a process critical to both neuronal migration and axon outgrowth. PMID: 16387638
    19. Doublecortin-like kinase (Dclk) gene functions in a partially redundant pathway with Dcx in the formation of axonal projections across the midline and migration of cortical neurons. PMID: 16387639
    20. unique role of the DCLK gene in mitotic spindle integrity during early neurogenesis PMID: 17313568
    21. In the embryonic brain, virtually all of the cells expressing doublecortin-like kinase contained no stem-like cells. PMID: 17409237
    22. DCLK has a distinct role in cortical interneuron migration; Dclk distribution is different in mutant mice compared with wild-type controls. PMID: 17409252
    23. Doublecortin and CaM kinase-like-1 (DCAMKL-1) was used to examine radiation-induced stem cell apoptosis and adenomatous polyposis coli (APC)/multiple intestinal neoplasia (min) mice to determine the effects of APC mutation on DCAMKL-1 expression. PMID: 18055444
    24. Interestingly, Dclk1 was expressed in CA3 neurons, including in the heterotopic pyramidal layer of Dcx knockout animals, but is presumably not able to compensate for a lack of Dcx. PMID: 18075264
    25. The microtubule-associated proteins doublecortin and doublecortin-like are differentially expressed, particularly during early corticogenesis, consistent with their different functional roles. PMID: 18231966
    26. The microtubule associated protein Doublecortin-like regulates the transport of the glucocorticoid receptor in neuronal progenitor cells. PMID: 17975023
    27. Doublecortin-like, a slice variant of DCLK1 and a microtubule-associated protein expressed in radial glia, is crucial for neuronal precursor division and radial process stability. PMID: 17313568
    28. Two splice variants of the DCLK gene are differentially expressed in areas of the adult hippocampus and display functional differences PMID: 14741399
    29. Multiple transcripts generated by the DCAMKL gene are expressed in the rat hippocampus PMID: 11597766
    30. DCLK is a physiological substrate for activated caspases. Cleavage of DCLK is necessary for neuronal apoptosis to proceed. PMID: 11479289
    31. DCLK is a physiological substrate for the cysteine protease calpain. In neurons cleavage of DCLK by calpain represents a calcium responsive mechanism to regulate localization of the DCLK kinase domain PMID: 11473121

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  • 蛋白家族：
    Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CaMK subfamily
  • 数据库链接：

    KEGG: mmu:13175

    STRING: 10090.ENSMUSP00000050034

    UniGene: Mm.393242