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  4. Recombinant Mouse Serine protease HTRA1 (Htra1), partial

Recombinant Mouse Serine protease HTRA1 (Htra1), partial

In Stock
  • 货号：
    CSB-EP879501MO1
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP879501MO1 could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Htra1.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP879501MO1 could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Htra1.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    Htra1
  • Uniprot No.：
  • 别名：
    Htra1; Htra; Prss11; Serine protease HTRA1; EC 3.4.21.-; High-temperature requirement A serine peptidase 1; Serine protease 11
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    39.0 kDa
  • 表达区域：
    141-480aa
  • 氨基酸序列
    KLRQPPVIVLQRGACGQGQEDPNSLRHKYNFIADVVEKIAPAVVHIELYRKLPFSKREVPVASGSGFIVSEDGLIVTNAHVVTNKNRVKVELKNGATYEAKIKDVDEKADIALIKIDHQGKLPVLLLGRSSELRPGEFVVAIGSPFSLQNTVTTGIVSTTQRGGKELGLRNSDMDYIQTDAIINYGNSGGPLVNLDGEVIGINTLKVTAGISFAIPSDKIKKFLTESHDRQAKGKAVTKKKYIGIRMMSLTSSKAKELKDRHRDFPDVLSGAYIIEVIPDTPAEAGGLKENDVIISINGQSVVTANDVSDVIKKENTLNMVVRRGNEDIVITVIPEEIDP
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    C-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer，50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The process of expressing the recombinant mouse Htra1 protein in the E.coli requires the recombinant DNA gene formed by the integration of encoding gene for the 141-480aa of the mouse Htra1 protein and C-terminal 6xHis tag sequence, the expression vector that the recombinant DNA gene inserts into, the E.coli that provided the necessary macromolecules and components for transcription and translation of the cloned expression vector. After isolation and purification, this C-terminal 6xHis-tagged recombinant Htra1 protein was obtained. This recombinant Htra1 protein is characterized by high purity (>85%, SDS-PAGE). This Htra1 protein ran along the gel to the band of approximately 43 kDa molecular weight.

    Serine protease HTRA1 is a protein encoding by a gene named Htra1 in mouse and a gene named HTRA1 in human. This protein, a member of serine proteases, which has an active center that cuts (cleaves) other proteins into smaller pieces. It consists of four well-defined domains, including an IGFBP domain, a Kazal domain, a protease domain and a PDZ domain. HTRA1 is a secretory protein and also present intracellularly and associated with microtubules. The proteolytic activity of HTRA1 regulates a broad range of physiological processes involving in bone biology, osteoarthritis, intervertebral disc (IVD) degeneration and tumorigenesis.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, but it is unclear whether it leads to the proteolytic degradation of TGF-beta proteins themselves or not. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets.
  • 基因功能参考文献：
    1. transfection of cells with a constitutively active rapamycin-resistant p70S6K mutant could restore the mineralizing capacity of HtrA1-deficient mouse adipose-derived stromal cells. PMID: 26950191
    2. HtrA1 plays important roles in the differentiation of trophoblasts from Tpbpa-positive precursors in the ectoplacental cone PMID: 25446274
    3. The HtrA1 overexpression and mainstream cigarette smoke can independently lead to CNV. The HtrA1 gene is a strong risk factor for wet AMD PMID: 25205867
    4. the inhibitory effect of IFN-gamma on HTRA1 expression was evidenced in collagen-induced arthritis (CIA) mouse models and in human RA synovial cells. PMID: 24907345
    5. Serine protease HTRA1 antagonizes transforming growth factor-beta signaling by cleaving its receptors and loss of HTRA1 in vivo enhances bone formation. PMID: 24040176
    6. This study offers new insights into the regulation of HTRA-1 expression via LPS/TLR-4 and the role of HTRA-1 in rheumatoid arthrisis pathogenesis. PMID: 23982886
    7. suggest a critical role for LRP1 in maintaining the integrity of vessels by regulating protease activity as well as matrix deposition by modulating HtrA1 and connective tissue growth factor protein levels. PMID: 23868935
    8. a critical role of HTRA1 in the regulation of angiogenesis via TGF-beta signaling and identified GDF6 as a novel disease gene for AMD. PMID: 22049084
    9. showed that increased HTRA1 induced cardinal features of polypoidal choroidal vasculopathy, including branching networks of choroidal vessels, polypoidal lesions, severe degeneration of the elastic laminae, and tunica media of choroidal vessels PMID: 21844367
    10. HtrA1 may disrupt the pericellular matrix network, resulting in alteration of chondrocyte metabolisms. This eventually leads to osteoarthritis. PMID: 20238298
    11. HtrA1 has a role in promoting anoikis by attenuating activation of EGFR/AKT pathway that may contribute to its metastasis suppression capacity PMID: 20388781
    12. During embryo development, HtrA1 was expressed in specific areas where signaling by Tgfbeta family proteins plays important regulatory roles PMID: 14973287
    13. HtrA1 as a protease potentially important for trophoblast differentiation/invasion and uterine decidual regression during placental development PMID: 15861393
    14. bone matrix shows a high level of HtrA1 protein deposition akin to that of TGF-beta, suggesting a close functional interaction between TGF-beta and HtrA1 PMID: 15993670
    15. highly upregulated during mucosal mast cell differentiation PMID: 17292962
    16. HtrA1 and PRSS35 may be involved in ovulation and corpus luteum formation and regression, and that PRSS23 may play a role in follicular atresia PMID: 18566130

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  • 亚细胞定位：
    Cell membrane. Secreted. Cytoplasm, cytosol.
  • 蛋白家族：
    Peptidase S1C family
  • 组织特异性：
    In the brain, mainly expressed in cortical areas both in glial cells and neurons (at protein level). In bones, deposited in the matrix, with higher level in newly formed bone compared to fully calcified bone (at protein level). Also expressed in the tendo
  • 数据库链接：

    KEGG: mmu:56213

    STRING: 10090.ENSMUSP00000006367

    UniGene: Mm.30156