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  4. Recombinant Mouse Serine/arginine-rich splicing factor 2 (Srsf2)

Recombinant Mouse Serine/arginine-rich splicing factor 2 (Srsf2)

  • 中文名称：
    小鼠Srsf2重组蛋白
  • 货号：
    CSB-YP730774MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Srsf2重组蛋白
  • 货号：
    CSB-EP730774MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Srsf2重组蛋白
  • 货号：
    CSB-EP730774MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Srsf2重组蛋白
  • 货号：
    CSB-BP730774MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Srsf2重组蛋白
  • 货号：
    CSB-MP730774MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Srsf2
  • Uniprot No.：
  • 别名：
    Srsf2; Pr264; Sfrs10; Sfrs2; Serine/arginine-rich splicing factor 2; Protein PR264; Putative myelin regulatory factor 1; MRF-1; Splicing component; 35 kDa; Splicing factor SC35; SC-35; Splicing factor; arginine/serine-rich 2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-221
  • 氨基酸序列
    SYGRPPPDV EGMTSLKVDN LTYRTSPDTL RRVFEKYGRV GDVYIPRDRY TKESRGFAFV RFHDKRDAED AMDAMDGAVL DGRELRVQMA RYGRPPDSHH SRRGPPPRRY GGGGYGRRSR SPRRRRRSRS RSRSRSRSRS RSRYSRSKSR SRTRSRSRST SKSRSARRSK SKSSSVSRSR SRSRSRSRSR SPPPVSKRES KSRSRSKSPP KSPEEEGAVS S
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. Can bind to the myelin basic protein (MBP) gene MB3 regulatory region and increase transcription of the mbp promoter in cells derived from the CNS. The phosphorylated form (by SRPK2) is required for cellular apoptosis in response to cisplatin treatment.
  • 基因功能参考文献：
    1. Posttranslational modification of SR proteins underlies the regulation of their mRNA export activities and distinguishes pluripotent from differentiated cells. PMID: 28592444
    2. Mice expressing Srsf2 mutation Srsf2P95H, which commonly occurs in individuals with MDS and AML, in an inducible, hemizygous manner in hematopoietic cells rapidly succumbed to fatal bone marrow failure, demonstrating that Srsf2-mutated cells depend on the wild-type Srsf2 allele for survival. PMID: 27135740
    3. Findings have shown that SRSF2 not only is involved in splicing regulation of a large number of transcripts but also activates transcription of metabolism-related genes and transcription factors. PMID: 27022105
    4. Depletion of SRSF2 enhances reovirus replication and cytopathic effect, suggesting that T1L mu2 modulation of splicing benefits the virus. PMID: 28077658
    5. HIV-1-Tat Protein Inhibits SC35-mediated Tau Exon 10 Inclusion through Up-regulation of DYRK1A Kinase. PMID: 26534959
    6. The findings suggest that intact SRSF2 is essential for the functional integrity of the hematopoietic system and that its mutations likely contribute to development of myelodysplastic syndromes. PMID: 26124281
    7. SRSF2 mutations alter SRSF2's normal sequence-specific RNA binding activity, thereby altering the recognition of specific exonic splicing enhancer motifs to drive recurrent mis-splicing of key hematopoietic regulators PMID: 25965569
    8. Study reports that SRSF2 (also known as SC35, an SR-splicing factor) is part of the 7SK complex assembled at gene promoters and plays a direct role in transcription pause release. PMID: 23663783
    9. Specific effects on regulated splicing by SR proteins SRSF1 and SRSF2 depends on a complex set of relationships with multiple other SR proteins in mammalian genomes. PMID: 23562324
    10. Deficiency of the splicing factor Sfrs10 results in early embryonic lethality in mice and has no impact on full-length SMN/Smn splicing. PMID: 20190275
    11. This protein was more abundant in the nucleoplasm of mice fed genetically-modified soybeans than in control mice. PMID: 12441651
    12. Oct-4 distribution was examined relative to that of the unphosphorylated form of RNA polymerase II (Pol II) and splicing factor (SC 35) in the intranuclear entities such as perichromatin fibrils. PMID: 12858338
    13. intranuclear distribution of SC35 is dependent on GSK-3 PMID: 14602710
    14. These findings reveal the involvement of SC35 in specific pathways in regulating cell proliferation and genomic stability during mammalian organogenesis and suggest its potential function in tumorigenesis. PMID: 17526736
    15. splicing factor SC35 has an active role in transcriptional elongation PMID: 18641664

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  • 亚细胞定位：
    Nucleus. Nucleus, nucleoplasm. Nucleus speckle.
  • 蛋白家族：
    Splicing factor SR family
  • 组织特异性：
    Expressed in all the tissues examined; liver, kidney, spleen, heart, lung and brain.
  • 数据库链接：

    KEGG: mmu:20382

    STRING: 10090.ENSMUSP00000090059

    UniGene: Mm.21841