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  4. Recombinant Mouse Semaphorin-4C (Sema4c), partial

Recombinant Mouse Semaphorin-4C (Sema4c), partial

  • 货号：
    CSB-YP723720MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP723720MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP723720MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP723720MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP723720MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Sema4c
  • Uniprot No.：
  • 别名：
    Sema4c; S4c; Semacl1; Semai; Semaphorin-4C; M-Sema F; Semaphorin-C-like 1; Sema I; Semaphorin I
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

What are the price of all size of the product CSB-MP723720MO in the version 6xhis tagged + My c tagged? Also I would like to know what is the protein sequence of these product? Is there any negative control available for these product?

A:
Thanks for your inquiry.
Express Region:21-500aa; Partial, contain Sema Domain
Tag information:EP,BP,MP:N-terminal 10xHis-tagged and C-terminal Myc-tagged；YP:N-terminal 6xHis-tagged
Express Sequence:

AEMWWNLVPRKTVSSGELVTVVRRFSQTGIQDFLTLTLTEHSGLLYVGAREALFAFSVEALELQGAISWEAPAEKKIECTQKGKSNQTECFNFIRFLQPYNSSHLYVCGTYAFQPKCTYINMLTFTLDRAEFEDGKGKCPYDPAKGHTGLLVDGELYSATLNNFLGTEPVILRYMGTHHSIKTEYLAFWLNEPHFVGSAFVPESVGSFTGDDDKIYFFFSERAVEYDCYSEQVVARVARVCKGDMGGARTLQKKWTTFLKARLVCSAPDWKVYFNQLKAVHTLRGASWHNTTFFGVFQARWGDMDLSAVCEYQLEQIQQVFEGPYKEYSEQAQKWARYTDPVPSPRPGSCINNWHRDNGYTSSLELPDNTLNFIKKHPLMEDQVKPRLGRPLLVKKNTNFTHVVADRVPGLDGATYTVLFIGTGDGWLLKAVSLGPWIHMVEELQVFDQEPVESLVLSQSKKVLFAGSRSQLVQLSLADC


As to the negative control, can you let me know which experiment will you use this protein? Usually we could use the protein's buffer as negative control.

靶点详情

  • 功能：
    Cell surface receptor for PLXNB2 that plays an important role in cell-cell signaling. PLXNB2 binding promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal brain development, axon guidance and cell migration. Probable signaling receptor which may play a role in myogenic differentiation through activation of the stress-activated MAPK cascade.
  • 基因功能参考文献：
    1. Follicular T helper cells use Semaphorin 4C as a guidance receptor to sense germinal center-expressed Plexin B2. PMID: 28467912
    2. we demonstrate that Sema4C is critical for optimal regulatory cytokine production in CD138(+) B cells. PMID: 27881703
    3. Sema4C-Plexin B2 signalling regulates ureteric branching PMID: 21035938
    4. We have identified here Sema4C and Sema4G as candidate ligands for Plexin-B2 PMID: 21122816
    5. Plexin-B2 and Sema4C are potential regulators of the vascular and endocrine system. PMID: 20478304
    6. Results suggest a putative role of Sema4C during neurogenesis both in vivo and in vitro. PMID: 19189244
    7. Sema4C-mediated interaction among myoblasts plays an important role in terminal myogenic differentiation PMID: 15811348
    8. Sema4C promotes terminal myogenic differentiation in a p38 MAPK-dependent manner. PMID: 17498836

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  • 亚细胞定位：
    Cell junction, synapse, postsynaptic density membrane; Single-pass type I membrane protein. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Semaphorin family
  • 组织特异性：
    Predominantly expressed in brain (at protein level).
  • 数据库链接：

    KEGG: mmu:20353

    STRING: 10090.ENSMUSP00000027295

    UniGene: Mm.29558