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  4. Recombinant Mouse Semaphorin-3A (Sema3a)

Recombinant Mouse Semaphorin-3A (Sema3a)

  • 货号：
    CSB-YP020980MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP020980MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP020980MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP020980MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP020980MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Sema3a
  • Uniprot No.：
  • 别名：
    Sema3a; Semad; SemD; Semaphorin-3A; Semaphorin III; Sema III; Semaphorin-D; Sema D
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    21-772
  • 氨基酸序列
    NYANGKNNVP RLKLSYKEML ESNNVITFNG LANSSSYHTF LLDEERSRLY VGAKDHIFSF NLVNIKDFQK IVWPVSYTRR DECKWAGKDI LKECANFIKV LEAYNQTHLY ACGTGAFHPI CTYIEVGHHP EDNIFKLQDS HFENGRGKSP YDPKLLTASL LIDGELYSGT AADFMGRDFA IFRTLGHHHP IRTEQHDSRW LNDPRFISAH LIPESDNPED DKVYFFFREN AIDGEHSGKA THARIGQICK NDFGGHRSLV NKWTTFLKAR LICSVPGPNG IDTHFDELQD VFLMNSKDPK NPIVYGVFTT SSNIFKGSAV CMYSMSDVRR VFLGPYAHRD GPNYQWVPYQ GRVPYPRPGT CPSKTFGGFD STKDLPDDVI TFARSHPAMY NPVFPINNRP IMIKTDVNYQ FTQIVVDRVD AEDGQYDVMF IGTDVGTVLK VVSVPKETWH DLEEILLEEM TVFREPTTIS AMELSTKQQQ LYIGSTAGVA QLPLHRCDIY GKACAECCLA RDPYCAWDGS SCSRYFPTAK RRTRRQDIRN GDPLTHCSDL QHHDNHHGPS LEERIIYGVE NSSTFLECSP KSQRALVYWQ FQRRNEDRKE EIRMGDHIIR TEQGLLLRSL QKKDSGNYLC HAVEHGFMQT LLKVTLEVID TEHLEELLHK DDDGDGSKIK EMSSSMTPSQ KVWYRDFMQL INHPNLNTMD EFCEQVWKRD RKQRRQRPGH SQGSSNKWKH MQESKKGRNR RTHEFERAPR SV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a role in growth cones guidance. May function to pattern sensory projections by selectively repelling axons that normally terminate dorsally. Involved in the development of the olfactory system and in neuronal control of puberty.
  • 基因功能参考文献：
    1. These findings indicate a regulatory mechanism and a proapoptotic function of aberrant Sema3A signaling in Osteoarthritis (OA) chondrocytes, and suggest that targeting Sema3A signaling might interfere OA pathogenesis. PMID: 29111592
    2. study demonstrates that Sema3E/plexinD1 inhibits proliferation and migration of vascular smooth muscle cells via inactivation of Rap1-AKT signalling pathways PMID: 29016743
    3. SEMA3A expression is increased in the endothelin receptor-B null mouse model of Hirschsprung disease, suggesting that SEMA3A may interfere with enteric neural crest cell migration, resulting in an absence of enteric neurons. PMID: 29224790
    4. Our results demonstrated that overexpression of Sema3A in oral cancer cells drastically suppressed tumor growth by inhibiting angiogenesis. PMID: 28683823
    5. a novel role for Sema3A-neuropilin 1 signalling in progenitor cell dynamics and in the generation of interneurons in the ventral telencephalon PMID: 27858201
    6. Sema3A reduces cardiac inflammation and improves cardiac function after myocardial infarction by promoting the resolution of inflammation. PMID: 28540528
    7. The findings highlight an active role for satellite cell-secreted Sema3A ligand as a key "commitment factor" for the slow-fiber population during muscle regeneration. PMID: 28480592
    8. This approach can be used to study responses to repulsive guidance factors such as semaphorin 3A. Here I describe the procedures used to prepare cultured neurons for rotary-shadow EM, allowing detailed comparisons of cytoskeletal structure PMID: 27787851
    9. Sema3a-Nrp2 signaling along an unrecognized pancreatic developmental axis constitutes a chemoattractant system essential for generating the hallmark morphogenetic properties of pancreatic islets. PMID: 28893946
    10. These data suggest that both Nrp1 and Sema3a are expressed in the cochlea during time points when spiral ganglion cell begin to innervate the organ of Corti. PMID: 29059194
    11. We show that PTP-3, a LAR homolog in Caenorhabditis elegans, participates in Sema2A-regulated axon guidance. PTPdelta, a member of vertebrate LAR class PTPs, is involved in Sema3A-regulated cortical dendritic growth. In Sema3A signaling, PTPdelta activates Fyn and Src kinases by dephosphorylating their C-terminal Tyr residues. PMID: 28637841
    12. Investigated the effects of BotoxA-induced paralysis on the sprouting capacity of neuromuscular junctions (NMJs) in the K108N-SEMA3A mutant, and observed no change in the differential neuronal plasticity found at NMJs on fast-fatigable or slow muscle fibers due to the presence of the SEMA3A mutant protein. PMID: 28103314
    13. The aim was to elucidate whether ectopic muscles are a result of misguidance of myoblast precursors due to the loss of Sema3A-Npn-1 signaling. PMID: 27466379
    14. These findings suggest that by interacting with PlexA4, TrkA plays a crucial role in redirecting local Sema3A signaling to retrograde axonal transport, thereby regulating dendritic GluA2 localization and patterning. PMID: 26945060
    15. Findings indicate that Rho and ROCK knockout may improve the behavior of mice and prevent MPTP-induced dopaminergic neurons damage by regulating Sema3A, PlexinA and NRP-1 in a mouse model of Parkinson's disease. PMID: 27772760
    16. Sema3A signaling exerts its oncogenic effect by promoting an mTORC1-mediated metabolic shift from oxidative phosphorylation to aerobic glycolysis. PMID: 27246732
    17. Semaphorin 3A (Sema3A) is a protein secreted during the development of the nervous system that acts as a cue for axon induction . PMID: 28013333
    18. this paper shows that Nav1.7, by coupling with CRMP1, mediates the axonal retrograde signaling of Sema3A in axonal guidance PMID: 28254884
    19. miR-30c and sema3A regulate adult neurogenesis by controlling proliferation and differentiation of stem cells in the subventricular zone. PMID: 27198082
    20. These findings indicate that endothelial cell-derived Sema3A exerts repelling functions on VEGF-A-induced tip cell filopodia and that a lack of this signaling cannot be rescued by paracrine sources of Sema3A. PMID: 26884395
    21. Data suggest that sema3A is involved in the pathogenesis of experimental autoimmune encephalomyelitis, in the modulation of the immune response and in the neurodegeneration that take place in the CNS. PMID: 26686462
    22. these studies demonstrated that Sema3A is a key mediator of cerebrovascular permeability and contributes to brain damage caused by cerebral ischemia. PMID: 25601765
    23. Diabetes induced sema3A excretion in urine. Increased semaphorin 3A was associated with severity of albuminuria. Seme3A-mediated diabetes induced glomerulosclerosis. PMID: 25249008
    24. These findings indicate that excess sema3a promotes severe diabetic nephropathy and identifies novel potential therapeutic targets for diabetic nephropathy. PMID: 25475434
    25. interleukin-4 and tumor necrosis factor-alpha stimulation increases epidermal innervation and suppresses semaphorin 3A PMID: 25088775
    26. The Sema3A/Npn1 pathway participates to the parcellation of the visual neocortex in both the mouse. PMID: 23709645
    27. Data suggest that loss of semaphorin-3A (Sema3A)-neuropilin 1 (Npn1) signaling is not the only cause for the developmental deficits. PMID: 25512301
    28. Sema3A is an essential, locally produced chemorepellant, which by creating mesenchymal exclusion areas, regulates the timing and patterning of the dental nerves during the development of incisor tooth germ. PMID: 24752460
    29. Sema3A exacerbates acute kidney injury via promoting inflammation and epithelial cell apoptosis. PMID: 24829504
    30. Blockade of Sema3A signaling via lens removal or injection of a synthetic Sema3A inhibitor causes ectopic migration of angioblasts into the cornea and results in its subsequent vascularization. PMID: 24809797
    31. This study examines the hypothesis that Sema3A signaling through its motor neuron neuropilin-1 (NRP1) receptor triggers distal axonopathy PMID: 24918638
    32. genetic findings demonstrate that Sema3a repellent signaling plays a role in the establishment of proper afferent projections in SAG neurons, and this signaling likely occurs through a receptor complex involving Npn1 and either plexinA1 or plexinA3 PMID: 23991118
    33. these results suggest that Sema3A and Sema7A have opposite effects and are involved in different stages of synapse elimination. PMID: 24831527
    34. A novel direct interaction between the Sema3a signaling receptor plexinA1 and nephrin, linking extracellular Sema3a signals to the slit-diaphragm signaling complex, was identified. PMID: 23954273
    35. Sema3A is robustly induced in the early stages of diabetes and mediates, via Neuropilin-1, the breakdown of the inner blood-retinal barrier. PMID: 24093675
    36. Semaphorin 3A is expressed in lymphatic vessels and interacts with neuropilin 1 in lymphangiogenesis [review] PMID: 24276892
    37. Increased Levels of Axon Guidance Errors are Detected in Sema3A+/+:BAX-/- and Sema3A+/-:BAX-/- Mice. PMID: 23922915
    38. Sema3A may play a key role in the proper development of the dorsal ramus projection. PMID: 23998265
    39. shows that TAMs' heterogeneity depends on their localization, which is tightly controlled by Sema3A/Nrp1 signaling PMID: 24332039
    40. results demonstrate a critical role of local SEMA3A signaling in layer-specific axonal branching, which contributes to target innervation PMID: 23602477
    41. Studied the therapeutic effect of endostar with cisplatin and investigated the inhibiting mechanism of endostar from tumor angiogenesis. Expressions of VEGF and Sema3A were evaluated by immunhistochemisty. PMID: 23317887
    42. study demonstrates that Sema3A regulates bone remodelling indirectly by modulating sensory nerve development, but not directly by acting on osteoblasts PMID: 23644455
    43. Sema3A overrides the effects of Sema3C in cortical axons exposed to defined mixtures of these opposing guidance cues in vitro PMID: 22368082
    44. Semaphorin 3A impinged upon the basal activity of the serine phosphatase PP2A and disrupted PP2A interaction with VE-cadherin, leading to cell-cell junction disorganization and increased permeability. PMID: 22685328
    45. These findings indicate a critical role for Sema3-Nrp1 signaling in fetal pulmonary development. PMID: 23063659
    46. There was an approximately 40% increase in the number of branching points and in the length of the dendrites in Sema3A-treated CRMP4+/+ hippocampal neurons. PMID: 22234963
    47. Following middle cerebral artery occlusion to induce stroke, immunohistochemistry shows both Sema3A and 12/15-LOX are increased in the cortex up to 2 wk. PMID: 23070608
    48. Expression patterns of Sema3A indicate that it may control the timing and patterning of the incisor innervation. Sema3A appears to regulate innervation of the periodontal ligament. PMID: 21793640
    49. Data demonstrate an essential direct function of Sema3A-Nrp1-PlexinA1 signaling in lymphatic valve formation. PMID: 22723296
    50. Sema3A-Nrp-1 signaling directs the maturation of the lymphatic vascular network likely via regulating the perivascular cell coverage of the vessels thus affecting lymphatic vessel function and lymphatic valve development. PMID: 22723300

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Semaphorin family
  • 数据库链接：

    KEGG: mmu:20346

    STRING: 10090.ENSMUSP00000030714

    UniGene: Mm.372039