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  4. Recombinant Mouse Segment polarity protein dishevelled homolog DVL-1 (Dvl1)

Recombinant Mouse Segment polarity protein dishevelled homolog DVL-1 (Dvl1)

  • 货号：
    CSB-YP007284MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP007284MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP007284MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP007284MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP007284MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Dvl1; DvlSegment polarity protein dishevelled homolog DVL-1; Dishevelled-1; DSH homolog 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-695
  • 氨基酸序列
    MAETKIIYHM DEEETPYLVK LPVAPERVTL ADFKNVLSNR PVHAYKFFFK SMDQDFGVVK EEIFDDNAKL PCFNGRVVSW LVLAEGAHSD AGSQGTDSHT DLPPPLERTG GIGDSRPPSF HPNVASSRDG MDNETGTESM VSHRRERARR RNRDEAARTN GHPRGDRRRD LGLPPDSAST VLSSELESSS FIDSDEEDNT SRLSSSTEQS TSSRLVRKHK CRRRKQRLRQ TDRASSFSSI TDSTMSLNII TVTLNMERHH FLGISIVGQS NDRGDGGIYI GSIMKGGAVA ADGRIEPGDM LLQVNDVNFE NMSNDDAVRV LREIVSQTGP ISLTVAKCWD PTPRSYFTIP RADPVRPIDP AAWLSHTAAL TGALPRYGTS PCSSAITRTS SSSLTSSVPG APQLEEAPLT VKSDMSAIVR VMQLPDSGLE IRDRMWLKIT IANAVIGADV VDWLYTHVEG FKERREARKY ASSMLKHGFL RHTVNKITFS EQCYYVFGDL CSNLASLNLN SGSSGASDQD TLAPLPHPSV PWPLGQGYPY QYPGPPPCFP PAYQDPGFSC GSGSAGSQQS EGSKSSGSTR SSHRTPGREE RRATGAGGSG SESDHTVPSG SGSTGWWERP VSQLSRGSSP RSQASAVAPG LPPLHPLTKA YAVVGGPPGG PPVRELAAVP PELTGSRQSF QKAMGNPCEF FVDIM
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes. Required for LEF1 activation upon WNT1 and WNT3A signaling. DVL1 and PAK1 form a ternary complex with MUSK which is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ).
  • 基因功能参考文献：
    1. Data show that the peptide binding pocket of the Dishevelled 1 (Dvl1) PDZ domain is stabilized upon CXXC finger 5 protein (CXXC5) binding. PMID: 27932247
    2. Here we show that the peptide-binding pocket of the Dvl PDZ domain can be occupied by Dvl's own highly conserved C-terminus, inducing a closed conformation. PMID: 26297804
    3. The interaction of Dvl1 with Syt-1, which is regulated by Wnts, modulates neurotransmitter release. PMID: 26400647
    4. High disheveled expression is associated with pulmonary fibrosis. PMID: 25445788
    5. findings indicate that Dvl-1 may be an underlying participant of oxidative stress induced by selenium deficiency PMID: 24234591
    6. These results support a novel cell-autonomous postsynaptic role for Dact1, in cooperation with Dishevelled-1 and possibly Disrupted in Schizophrenia-1, in the formation of synapses on cortical interneuron dendrites. PMID: 23826333
    7. we show that Dvl-1 expression is restricted to OSNs in the dorsal recess of the nasal cavity, and labels a unique subpopulation of glomeruli. Dvl-2 and Dvl-3 have a widespread distribution in both the olfactory epithelium and olfactory bulb . PMID: 23437169
    8. Fuzzy appears to control subcellular localization of the core PCP protein Dishevelled, recruiting it to Rab8-positive vesicles and to the basal body and cilium. We show that loss of Fuzzy results in inhibition of PCP signaling PMID: 23303251
    9. A Wnt5a--> Dvl PCP signaling cascade may regulate actin polymerization and protrusive cell behavior in the caudal splanchnic mesoderm to promote second heart field deployment, outflow tract lengthening and cardiac looping. PMID: 22841628
    10. Findings indicate that Daple interacts with Dishevelled to direct the Dishevelled/protein kinase lambda protein complex to activate Rac, which in turn mediates the non-canonical Wnt signalling pathway required for cell migration. PMID: 22643886
    11. The antagonistic functions of Vangl2 and Dvl1 allow sharpening of planar cell polarity signaling locally on the tips of the filopodia to sense directional cues, Wnts, eventually causing turning of growth cones. PMID: 21316586
    12. TMEM88 associates with Dvl proteins and regulates Wnt signaling in a context-dependent manner PMID: 21044957
    13. phosphorylation of Dvl triggers negative feedback regulation for different branches of Wnt signaling in a Ror2-dependent manner. PMID: 20215527
    14. Loss of CYLD instigates tumor growth in human cylindromatosis through a mechanism in which hyperubiquitination of polymerized Dvl drives enhancement of Wnt3/beta-catenin responses. PMID: 20227366
    15. preliminary characterization of it's interaction with Smad3 PMID: 12650946
    16. Data show that Dishevelled (Dvl) binding peptides of Frizzled inhibited canonical Wnt signaling and blocked Wnt-induced secondary axis formation in a dose-dependent manner, but did not block noncanonical Wnt signaling. PMID: 14636582
    17. The specificity of observed social interaction deficits suggests that lack of Dvl1 is associated with deficits in the recognition of social hierarchy and dominance. The sensorimotor gating deficit was subject to environmental influences. PMID: 14960015
    18. Results suggest that casein kinase Iepsilon functions as a molecular switch to direct Dishevelled (Dvl) from the c-Jun N-terminal kinase pathway to the beta-catenin pathway, possibly by altering the conformation of the C terminus of Dvl. PMID: 14966280
    19. DEP domain is responsible for the membrane translocation of Dvl-1 protein upon Wnt signal stimulation. PMID: 15353129
    20. Dvl induces cytoskeletal and morphologic rearrangements in N2A cells through a mechanism that cannot be attributed exclusively to modulation of GSK3beta or beta-catenin activity, but which depends upon a DIX-domain dependent signaling pathway. PMID: 15548427
    21. We identify dishevelled as a key player in transducing both Wnt canonical and non-canonical signals in dopaminergic cells. PMID: 15748172
    22. These results indicate that Dishevelled-1 may be involved in spermatid morphological changes during mouse spermiogenesis through mediating signal transduction and/or regulating actin cytoskeleton organization. PMID: 16541447
    23. Dvl1 mediates a conserved mammalian PCP pathway to regulate convergent extension during neurulation. PMID: 16571627
    24. Both Par1b and Dvl1 function antagonistically to regulate the assembly of functional E-cadherin-dependent adhesion complexes. PMID: 16707567
    25. We show that loss of function of Dishevelled-1 (Dvl1) mimics and enhances the Wnt7a phenotype in the cerebellum.Wnt7a/Dvl1 double mutant mice reveal a defect in neurotransmitter release at mossy fiber-granule cell synapses PMID: 16818724
    26. data provide evidence for a molecular mechanism that could be a basis for a novel negative feedback loop within the Wnt/Frizzled pathway depending on dishevelled degradation PMID: 17428233
    27. The expression of Dishevelleds in mammalian cells provide an estimate of the relative cellular abundance of each Dvl. PMID: 18093802
    28. a carboxyl-terminal binding partner, Dvl, has a role in activation of Daam1 PMID: 18162551
    29. Dvl1, Dvl2 and Dvl3 are functionally redundant in cardiac outflow tract, cochlea, and neural tube development. PMID: 19008950

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  • 亚细胞定位：
    Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytosol. Cytoplasmic vesicle. Note=Localizes at the cell membrane upon interaction with frizzled family members.
  • 蛋白家族：
    DSH family
  • 组织特异性：
    High levels are seen in the brain, testis and kidney, lower levels in the ovary, breast, muscle, liver and small intestine, and very low levels are seen in the spleen and thymus. A moderate level expression is seen in the heart.
  • 数据库链接：

    KEGG: mmu:13542

    STRING: 10090.ENSMUSP00000030948

    UniGene: Mm.3400