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  4. Recombinant Mouse Rho GDP-dissociation inhibitor 1 (Arhgdia)

Recombinant Mouse Rho GDP-dissociation inhibitor 1 (Arhgdia)

  • 货号：
    CSB-YP860418MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP860418MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP860418MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP860418MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP860418MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Arhgdia; C87222; Gdi1Rho GDP-dissociation inhibitor 1; Rho GDI 1; GDI-1; Rho-GDI alpha
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-204
  • 氨基酸序列
    AEQEPTAEQ LAQIAAENEE DEHSVNYKPP AQKSIQEIQE LDKDDESLRK YKEALLGRVA VSADPNVPNV IVTRLTLVCS TAPGPLELDL TGDLESFKKQ SFVLKEGVEY RIKISFRVNR EIVSGMKYIQ HTYRKGVKID KTDYMVGSYG PRAEEYEFLT PMEEAPKGML ARGSYNIKSR FTDDDKTDHL SWEWNLTIKK EWKD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Controls Rho proteins homeostasis. Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Retains Rho proteins such as CDC42, RAC1 and RHOA in an inactive cytosolic pool, regulating their stability and protecting them from degradation. Actively involved in the recycling and distribution of activated Rho GTPases in the cell, mediates extraction from membranes of both inactive and activated molecules due its exceptionally high affinity for prenylated forms. Through the modulation of Rho proteins, may play a role in cell motility regulation. In glioma cells, inhibits cell migration and invasion by mediating the signals of SEMA5A and PLXNB3 that lead to inactivation of RAC1.
  • 基因功能参考文献：
    1. GDIalpha has a critical role in suppressing Rac1 activity in mature podocytes PMID: 26726844
    2. The siRNA-mediated suppression of expression of alphaGDI especially affected vesicle dynamics. PMID: 26971292
    3. Integrin beta 8 and PTP-PEST form protein complexes at the leading edge of migrating cells and balance patterns of Rac1 and Cdc42 signaling by controlling the subcellular localization and phosphorylation status of Rho GDP dissociation inhibitor 1. PMID: 25666508
    4. RhoGDalpha controls ENaC expression and activity via Rac1. PMID: 25164814
    5. Results identify a critical role for 14-3-3tau in promoting breast cancer metastasis, in part through binding to and inhibition of RhoGDIalpha, a negative regulator of Rho GTPases and a metastasis suppressor. PMID: 24820414
    6. Data found that CCK-induced PKCalpha phosphorylation on RhoGDI1 at Ser96 releases RhoA and Rac1 from RhoGDI1 to facilitate Rho GTPases signaling. PMID: 23776598
    7. we demonstrated the regulation of targeting/accumulation of the RhoGDIalpha-Rac1 complex to phagosomes PMID: 23918979
    8. how the beta cell utilizes RhoGDI for differential Cdc42 and Rac1 cycling PMID: 20028975
    9. Morphological analysis of Rho GDP Dissociation Inhibitor (-/-) renal mesangial cells reveals a decrease in cell spreading compared to wild-type cells and a decreased ability to proliferate and survive. PMID: 19765647
    10. Inhibition of Rho family GTPases by Rho GDP dissociation inhibitor disrupts cardiac morphogenesis and inhibits cardiomyocyte proliferation. PMID: 11923206
    11. Increased expression of bovine Rho GDIalpha led to electrophysiological alterations that resulted in atrial arrhythmias and AV conduction abnormalities in transgenic mice, whereas ventricular contractile function was largely preserved. PMID: 15033930
    12. analysis of inhibitory and shuttling functions of rhoGDI-3 and rhoGDI-1 PMID: 15513926
    13. isoprenylcysteine carboxylmethyltransferase regulates Rac1 activity by controlling the interaction of Rac1 with RhoGDI through TNFA PMID: 15647276
    14. Rho GDIalpha and Rho GDIbeta play synergistic roles in lymphocyte migration and development by modulating activation cycle of the Rho proteins in a lymphoid organ-specific manner. PMID: 17142749
    15. RhoGDI-1 regulates lung microvessel endothelial barrier function by repressing RhoA activity. PMID: 17525371
    16. Differential expression of RhoGDI-1, cofilin 1 and tropomyosin 2 in calpain-deficient fibroblasts compared with wild-type fibroblasts PMID: 18076376
    17. liposomes supplemented with (PtdIns(3,4,5)P(3)) in conjunction with Rac, Trio, or Tiam1 and a non-hydrolyzable GTP analogue, cause dissociation of Rac1(GDP).RhoGDI complexes, GDP to GTP exchange on Rac1, and binding of Rac1(GTP) to the liposomes PMID: 18505730

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Rho GDI family
  • 组织特异性：
    In kidney glomerulus, expressed in podocytes and mesangial cells.
  • 数据库链接：

    KEGG: mmu:192662

    STRING: 10090.ENSMUSP00000063714

    UniGene: Mm.30016