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  4. Recombinant Mouse Regulator of G-protein signaling 7 (Rgs7)

Recombinant Mouse Regulator of G-protein signaling 7 (Rgs7)

  • 货号：
    CSB-YP019659MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP019659MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP019659MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP019659MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP019659MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Rgs7
  • Uniprot No.：
  • 别名：
    Rgs7; Regulator of G-protein signaling 7; RGS7
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-469
  • 氨基酸序列
    MAQGNNYGQT SNGVADESPN MLVYRKMEDV IARMQDEKNG IPIRTVKSFL SKIPSVFSGS DIVQWLIKNL TIEDPVEALH LGTLMAAHGY FFPISDHVLT LKDDGTFYRF QTPYFWPSNC WEPENTDYAV YLCKRTMQNK ARLELADYEA ESLARLQRAF ARKWEFIFMQ AEAQAKVDKK RDKIERKILD SQERAFWDVH RPVPGCVNTT EVDIKKSSRM RNPHKTRKSV YGLQNDIRSH SPTHTPTPET KPPTEDELHQ QIKYWQIQLD RHRLKMSKVA DSLLSYTEQY VEYDPFLVPP DPSNPWLSDD TTFWELEASK EPSQQRVKRW GFGMDEALKD PVGREQFLKF LESEFSSENL RFWLAVEDLK RRPIREVPSR VQEIWQEFLA PGAPSAINLD SKSYDKTTQN VKEPGRYTFE DAQEHIYKLM KSDSYPRFIR SSAYQELLQA KRKGKTLTSK RLTSLVQSY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. The RGS7/GNB5 dimer enhances GNAO1 GTPase activity. May play a role in synaptic vesicle exocytosis. Modulates the activity of potassium channels that are activated by GNAO1 in response to muscarinic acetylcholine receptor M2/CHRM2 signaling.
  • 基因功能参考文献：
    1. Stimulation of the ERK pathway in Gnb5(-/-) cells by epidermal growth factor restored M3R-stimulated insulin release to near normal levels. Identification of the novel role of Gbeta5-R7 in insulin secretion may lead to a new therapeutic approach for improving pancreatic beta-cell function PMID: 28687610
    2. This study identifies RGS7 as a novel regulator of Mu Opioid Receptor signaling by dissecting its circuit specific actions and pinpointing its role in regulating morphine reward by controlling the activity of nucleus accumbens neurons. PMID: 26364547
    3. R7-binding protein had a strong inhibitory effect on homo-oligomerization of RGS7. PMID: 26895961
    4. Association with the plasma membrane is sufficient for potentiating catalytic activity of RGS7 and RGS9-2 proteins. PMID: 26811338
    5. results demonstrate a novel role of RGS7 in hippocampal synaptic plasticity and memory formation PMID: 24755289
    6. GPR158 is an essential regulator of RGS7 in the native nervous system with a critical role in controlling its expression, membrane localization, and catalytic activity PMID: 25792749
    7. Expression of the Gbeta5/R7-RGS protein complex in pituitary and pancreatic islet cells. PMID: 22322946
    8. This study demonistrated that the subcellular localization of RGS7 in neurons and found that a significant fraction is present in cytoplasmic puncta. PMID: 22640015
    9. RGS7 and RGS11 are the key GTPase accelerating proteins in the mGluR6 pathway of retinal rod ON bipolar cells that set the sensitivity and time course of light-evoked responses. PMID: 22547806
    10. Defective retinal depolarizing bipolar cells in regulators of G protein signaling (RGS) 7 and 11 double null mice. PMID: 22371490
    11. R7BP contributes significantly to the nuclear localization of endogenous G beta(5)/regulator of G protein signaling 7 complex in brain PMID: 20100282
    12. study found that RGS7-Gbeta5 complexes bound to G protein-gated potassium channels and facilitated their functional coupling to GABA(B) receptors in neurons PMID: 20453851
    13. Delivery of RGS7/Gbeta5 to dendrites of ON-bipolar cells occurs independently of its association with R7BP. These findings provide a new mechanism for adapter-independent targeting of RGS/Gbeta5 complexes. PMID: 18842904
    14. RGS7 staining is present in dendritic tips of all rod ON-retinal bipolar cell. Genetic disruption of RGS7 produced delays in the ON-BPC-derived electroretinogram b-wave, but no changes in the photoreceptor-derived a-wave. PMID: 19535587

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  • 亚细胞定位：
    Cytoplasm, cytosol. Cytoplasm. Cell membrane. Membrane; Peripheral membrane protein; Cytoplasmic side.
  • 组织特异性：
    Detected in brain (at protein level).
  • 数据库链接：

    KEGG: mmu:24012

    STRING: 10090.ENSMUSP00000027812

    UniGene: Mm.7956