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  4. Recombinant Mouse Receptor-type tyrosine-protein phosphatase C (Ptprc), partial

Recombinant Mouse Receptor-type tyrosine-protein phosphatase C (Ptprc), partial

  • 货号：
    CSB-YP019049MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP019049MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP019049MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP019049MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP019049MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ptprc; Ly-5; Receptor-type tyrosine-protein phosphatase C; EC 3.1.3.48; Leukocyte common antigen; L-CA; Lymphocyte antigen 5; Ly-5; T200; CD antigen CD45
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.
  • 基因功能参考文献：
    1. Inhibition by high CD45 on CD8(+) T cells may protect against overt T-cell receptor (TCR) auto-MHC reactivity. PMID: 27841348
    2. this study shows that CD45 positioning within lipid rafts is modified during their oncogenic transformation to acute myeloid leukemia PMID: 27579617
    3. Data show that CD45(+) uncultured adipose tissue derived stromal cells (u-ADSCs) acting phenotypically and functionally like M2-type macrophages (MPhis), contributed to the repair of liver tissue undergoing inflammation. PMID: 28891216
    4. We identify differing roles for the phosphatase CD45 in innate and adaptive immune cells in intestinal inflammation. CD45 is required for optimal GM-CSF and RA production in innate immune cells that affects alpha4beta7 expression and the homing of effector T cells to the intestine. Conversely, the absence of CD45 on adaptive immune cells leads to enhanced alpha4beta7 expression on T cells and homing to the intestine. PMID: 27007678
    5. T cell receptor / TLR2 co-stimulation expands CD25+CD45Rbhi T cells. PMID: 28742882
    6. Increased numbers of CD4(+)CD45RA(-)FoxP3(low) cells may cause an imbalance between Treg and Th17 cells that is mainly localized to the colon. PMID: 27895423
    7. Use of the common leukocyte marker CD45 increases the sensitivity of the diagnosis of lymphocytic myocarditis. PMID: 28025077
    8. Aging induces loss of stemness with concomitant gain of myogenic properties of a pure population of CD34+/CD45- muscle derived stem cells. PMID: 26655331
    9. deletion of Pten in CD45-expressing cells induces development of T-cell acute lymphoblastic leukemia and lymphoma, but not other hematologic malignancies PMID: 26773036
    10. A noncatalytic role for CD45 in regulating tonic, but not antigen-mediated, B-cell antigen receptor (BCR) signaling through modulation of the function of the inhibitory coreceptor CD22, was identified. PMID: 26561584
    11. data demonstrate that the CD45E613R mutation modulates integrin activation and leukocyte recruitment during inflammation. PMID: 25505282
    12. CD45 ligation specifically reduced Treg motility in an integrin-dependent manner, resulting in enhanced interactions between Tregs and DCs in vivo. PMID: 25202978
    13. Data (including data from genome-wide mutagenesis studies) suggest that nonsense mutation in Ptprc is a susceptibility trait for herpes simplex encephalitis due to Herpesvirus 1; this effect appears to be mediated by Th1 cells and the brain stem. PMID: 24068938
    14. These findings demonstrate a novel role for CD45 on innate immune cells in promoting lymphopenia-induced T cell proliferation and suggest that innate immune cells may communicate with stromal cells to regulate IL-7 production. PMID: 25114101
    15. Expressing CD45 promoters containing these regions and tethered to green fluorescent protein (GFP) in a primary B-cell differentiation assay and a transplantation model resulted in high levels of GFP in lymphoid, myeloid, and nucleated erythroid cells PMID: 24852660
    16. identified a novel CD45 phosphorylation event in hematopoietic progenitor cells that correlated with motile response and governed by the chemotactic factor CXCL12 PMID: 23997015
    17. These data indicate that the alteration in the ratio of CD45.1 and CD45.2 B cell subsets in mixed chimera mice is a cell-intrinsic effect. PMID: 23928494
    18. The large ectodomains of CD45 and CD148 modulate their inhibitory effect by enabling their passive, size-based segregation from ligated TCR, supporting the kinetic-segregation model of TCR triggering. PMID: 23580664
    19. CD11c+B220+CD122+ cells play an important role in xenograft rejection. Their absence in NOG mice may be critical in supporting the successful engraftment of xenotransplants. PMID: 23018460
    20. CD19positive CD45R(lo)positive lymphocytes of embryonic origin are present in Peyer's patches and in the spleen throughout the life span of wild-type mice, beginning at postnatal day 7. PMID: 22837485
    21. Relative quantitative real time PCR (RT-PCR) of 64 genes revealed that mSPCD45(-) compared with mSPCD45(+) was enriched for cells expressing transcripts associated with endothelial cells, Notch signaling and myogenic precursors PMID: 21990046
    22. beta-defensin 14 expression by tumor-infiltrating host cells chemoattracts B220-positive lymphocytes in a CCR6-dependent manner, initiating angiogenesis and development of organized tumor tissue. PMID: 22504651
    23. These findings reveal how CD45 function diverges in T cells and B cells. PMID: 22135465
    24. CD45 and CD148 preferentially target different SFK members (Hck and Fgr versus Lyn, respectively) to positively and negatively regulate GPCR pathways. PMID: 22078799
    25. The pan-leukocyte CD45 phosphatase plays an essential role in trafficking and repopulation of the bone marrow by immature human CD34(+) cells and leukemic cells in transplanted nonobese diabetic severe combined immunodeficient mice. PMID: 21911094
    26. Syk, but not Zap70, controls the remaining CD45-independent, immunoreceptor tyrosine-based activation motif -specific NK-cell functions. PMID: 21245479
    27. CD45 expression was required for the modulation of T cell death and proliferation induced by galactoxylomannan. PMID: 20856869
    28. CD4-positiveCD45RB(low) cells and CD8-positiveCD122-positive regulatory T cells synergistically work to suppress colitis in a mouse model. PMID: 21098236
    29. Data show that CD45 deficiency affects the development and progression of DN1 thymocytes. PMID: 20624943
    30. The shift to high molecular weight CD45 isoforms in RNA-binding proteiin heterogeneous nuclear ribonucleoprotein L (Hnrpll) transgenic thymocytes does not demonstrably affect expression of CD45RA and -RC isoforms. PMID: 20505149
    31. CD45 regulates thymocyte survival during development in fetal thymic organ culture. PMID: 19765855
    32. show that relative titration of CD45 and Csk expression reveals distinct regulation of basal and inducible T cell receptor signaling during thymic development. PMID: 20346773
    33. regulation of galectin-1 signaling by alpha2,6-sialylation of N-glycans is not solely dependent on CD45 phosphatase activity PMID: 19920154
    34. These data indicate that CD45 is essential to the accumulation of CD4(-)/CD8(-) (DN) T cells in Fasl(gld/gld) mice PMID: 11716526
    35. Role of high and low molecular weight isoforms of CD45 in the function of naive and memory T lymphocytes. Review. PMID: 12022705
    36. The extracellular and/or the transmembrane portion of CD45 has a major role in apoptosis signaling which is independent of its phosphatase activity and association with leukocyte phosphatase-associated phosphoprotein. PMID: 12055218
    37. Characterization of a new subpopulation of mouse CD8alpha+ B220+ dendritic cells endowed with type 1 interferon production capacity and tolerogenic potential. PMID: 12091326
    38. CD22 binding to native CD45 PMID: 12115612
    39. Segregation of CD45 from proximal TCR signaling components is necessary for TCR signaling and that the targeting of CD45 PTP activity to lipid microdomains on the T cell surface results in decreased sensitivity of TCR-mediated signaling. PMID: 12207342
    40. CD45 is present in lipid rafts in T cells before activation, perhaps to activate raft-associated p56(lck), allowing membrane-proximal signaling events to proceed. PMID: 12391219
    41. the large CD45 ectodomain is required for optimal TCR signaling PMID: 12496963
    42. CD45 is regulated by ST6Gal I sialyltransferase in a process that leads to T cell death PMID: 12499376
    43. The inhibitory function of PAG in T cells is suppressed by CD45. PMID: 12612075
    44. the primary function of CD45 is inhibition of Src-PTKs and that the level of Src-PTK activation and the B-cell fate are determined in part by dynamic behavior of CD45 with respect to glycolipid microdomains PMID: 14563648
    45. CD45-D2 has a role in binding substrate and binds the SD10 region in Lck, which is a novel site involved in substrate recognition PMID: 14625311
    46. results support a model in which binding of CD45-AP to inactive CD45 dimers converts them to active monomers PMID: 14715639
    47. an increase in the percentage of CD45- multiple myeloma cells preceded the angiogenic switch PMID: 15070695
    48. de novo synthesis of CD45 results in a peanut agglutinin high phenotype in CD8+ T cells PMID: 15210702
    49. The characterization of T and B cell populations in the distinct compartments of the gut mucosa and gut-associated lymphoid tissue of CD45-null mice. PMID: 15557316
    50. A novel mechanism is described by which lipid raft-excluded CD45 positively regulates T cell receptor signal transduction and IL-2 production. PMID: 15661907

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Membrane raft.
  • 蛋白家族：
    Protein-tyrosine phosphatase family, Receptor class 1/6 subfamily
  • 数据库链接：

    KEGG: mmu:19264

    STRING: 10090.ENSMUSP00000027645

    UniGene: Mm.391573