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  4. Recombinant Mouse Ras-related protein M-Ras (Mras)

Recombinant Mouse Ras-related protein M-Ras (Mras)

  • 货号：
    CSB-YP014781MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP014781MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP014781MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP014781MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP014781MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Mras; Xras; Ras-related protein M-Ras; Muscle and microspikes Ras; Ras-related protein R-Ras3; X-Ras
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-205
  • 氨基酸序列
    MATSAVPSEN LPTYKLVVVG DGGVGKSALT IQFFQKIFVP DYDPTIEDSY LKHTEIDNQW AILDVLDTAG QEEFSAMREQ YMRTGDGFLI VYSVTDKASF EHVDRFHQLI LRVKDRESFP MILVANKVDL MHLRKVTRDQ GKEMATKYNI PYIETSAKDP PLNVDKTFHD LVRVIRQQVP EKNQKKKKKT KWRGDRATGT HKLQC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serves as an important signal transducer for a novel upstream stimuli in controlling cell proliferation. Activates the MAP kinase pathway.
  • 基因功能参考文献：
    1. This study demonstrated that M-Ras is recruited to the membrane of phagocytic cups and contributes to the regulation of phagosome formation in macrophages. PMID: 29340604
    2. Expression of the muscarinic M2 and M3 receptors that mediate the cholinergic contractile stimuli of the detrusor muscle was dysregulated in both Mras-/- males and females, although only males exhibited a urinary phenotype. PMID: 26516777
    3. Overexpression of MRAS in mouse embryonic stem cells leads to different cellular phenotypes. PMID: 23863483
    4. succeed in solving two crystal structures corresponding to state 1 and state 2 from a single Ras polypeptide, M-RasD41E, carrying an H-Ras-type substitution in residue 41 PMID: 21388959
    5. X-ray crystal structure analyses of a series of mutant H-Ras and M-Ras in complex with guanosine 5'-(beta,gamma-imido)triphosphate (GppNHp), representing various intermediate states of the transition, were determined. PMID: 20479006
    6. These results imply that M-Ras, induced and activated by BMP-2 signaling, participates in the osteoblastic determination, differentiation, and transdifferentiation under p38 MAPK and JNK regulation PMID: 19800879
    7. Expression of activated mutants of M-Ras was sufficient for oncogenic transformation of a murine mammary epithelial cell line PMID: 14961075
    8. Showed that multiple aspects of the transformed phenotype induced by activated G22V M-Ras in a murine mammary epithelial cell line scp-2 were dependent on an autocrine mechanism. PMID: 15140946
    9. Data describe the crystal structure of M-Ras in the GDP-bound and guanosine 5'-(beta,gamma-imido)triphosphate (Gpp(NH)p)-bound forms. PMID: 15994326
    10. Endogenous M-Ras was activated by several trophic factors in astrocytes, including epidermal growth factor (EGF), basic fibroblast growth factor, and hepatocyte growth factor. M-Ras activation by EGF was more sustained compared to prototypic Ras. PMID: 16980617
    11. These findings proved a crucial role of the cross-talk between two Ras-family GTPases M-Ras and Rap1, mediated by RA-GEF-2, in adhesion signaling. PMID: 17538012
    12. Conserved expression of Mras was observed in the nervous system both in mice and in ascidians. PMID: 18977283

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  • 亚细胞定位：
    Cell membrane; Lipid-anchor; Cytoplasmic side.
  • 蛋白家族：
    Small GTPase superfamily, Ras family
  • 数据库链接：

    KEGG: mmu:17532

    STRING: 10090.ENSMUSP00000035045

    UniGene: Mm.2045