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  4. Recombinant Mouse Rab5 GDP/GTP exchange factor (Rabgef1)

Recombinant Mouse Rab5 GDP/GTP exchange factor (Rabgef1)

  • 中文名称：
    小鼠Rabgef1重组蛋白
  • 货号：
    CSB-YP864373MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Rabgef1重组蛋白
  • 货号：
    CSB-EP864373MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Rabgef1重组蛋白
  • 货号：
    CSB-EP864373MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Rabgef1重组蛋白
  • 货号：
    CSB-BP864373MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Rabgef1重组蛋白
  • 货号：
    CSB-MP864373MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Rabgef1
  • Uniprot No.：
  • 别名：
    Rabgef1; Rabex5; Rab5 GDP/GTP exchange factor; Rabex-5
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-491
  • 氨基酸序列
    MSLKSERRGI HVDQSELLCK KGCGYYGNPA WQGFCSKCWR EEYHKARQRQ IQEDWELAER LQREEEEAFA SSQSSQGAQS LTFSKFEEKK TNEKTRKVTT VKKFFSASSR AGSKKEIQEA KAPSPSINRQ TSIETDRVTK EFIDFLKTFH KTGQEVYKQT KMFLEAMPYK RDLSIEEQSE CTQDFYQNVA ERMQTRGKVP PEKVEKIMDQ IEKHIMTRLY KFVFCPETTD DEKKDLAIQK RIRALHWVTP QMLCVPVNEE IPEVSDMVVK AITDIIEMDS KRVPRDKLAC ITRCSKHIFN AIKITKNEPA SADDFLPTLI YIVLKGNPPR LQSNIQYITR FCNPSRLMTG EDGYYFTNLC CAVAFIEKLD AQSLNLSQED FDRYMSGQTS PRKQESESWP PEACLGVKQM YKNLDLLSQL NERQERIMNE AKKLEKDLID WTDGIAKEVQ DIVEKYPLEI KPPNQPLAAI DSENVENDKL PPPLQPQVYA G
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Rab effector protein acting as linker between gamma-adaptin, RAB4A or RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates nucleotide exchange on RAB5A. Can act as a ubiquitin ligase.
  • 基因功能参考文献：
    1. AP-1/sigma1A-ArfGAP1-Rabex-5 complex formation leads to more endosomal Rabex-5 and enhanced, Rab5(GTP)-stimulated Vps34 PI3-kinase activity, which is essential for multivesicular body endosome formation. PMID: 27411398
    2. findings reveal a key role for RABGEF1 in dampening keratinocyte-intrinsic MYD88 signaling and sustaining epidermal barrier function PMID: 27820702
    3. Knockdown of RABEX-5 also inhibited wound healing, migration and the invasive abilities of gastric cancer cells. PMID: 25427001
    4. Kruppel-like factor 5 is indispensable for adhesion, homing, lodging and retention of haematopoietic stem cells and progenitors in the bone marrow through Rab5-dependent post-translational regulation of beta1/beta2 integrins PMID: 23552075
    5. Rab5 is required for phagocytosis of heat-inactivated Pseudomonas aeruginosa by macrophages. PMID: 23630954
    6. a novel mechanistic insight into how Rabex-5 regulates internalization and postendocytic trafficking of ubiquitinated L1 destined for lysosomal degradation. PMID: 22846990
    7. Data report the identification of Rab22 as a binding site on early endosomes for direct recruitment of Rabex-5 and activation of Rab5, establishing a Rab22-Rab5 signaling relay to promote early endosome fusion. PMID: 19759177
    8. RabGEF1 is a negative regulator of Fc epsilon RI-dependent mast cell activation, and a lack of RabGEF1 results in the development of skin inflammation in vivo. PMID: 15235600
    9. p85alpha protein may play a role in the down-regulation of activated receptors through its temporal control of the GTPase cycles of Rab5 and Rab4 PMID: 15377662
    10. RabGEF1 plays a critical role in the regulation of SCF/c-Kit-mediated signaling events and biological responses in mast cells. PMID: 16533754
    11. Data show that RabGEF1 is a negative regulator of Ras signalling and FcepsilonRI-dependent mast cell activation in vitro, and a lack of RabGEF1 results in the development of elevated numbers of mast cells in the skin and severe skin inflammation in vivo. PMID: 16605131
    12. Insulin-stimulated Interaction between insulin receptor substrate 1 and p85alpha and activation of protein kinase B/Akt require Rab5. PMID: 16880210
    13. Our data demonstrate that TeNT H(C) uses a retrograde transport pathway shared with p75(NTR), TrkB, and BDNF, which is strictly dependent on the activities of both Rab5 and Rab7 PMID: 17046692

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  • 亚细胞定位：
    Cytoplasm. Early endosome. Recycling endosome.
  • 组织特异性：
    Expressed in the white matter tracts of the cerebellum, the fimbria hippocampi and the corpus callosum.
  • 数据库链接：

    KEGG: mmu:56715

    STRING: 10090.ENSMUSP00000026390

    UniGene: Mm.288639