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  4. Recombinant Mouse RNA-binding protein Musashi homolog 2 (Msi2)

Recombinant Mouse RNA-binding protein Musashi homolog 2 (Msi2)

  • 中文名称：
    小鼠Msi2重组蛋白
  • 货号：
    CSB-YP846031MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Msi2重组蛋白
  • 货号：
    CSB-EP846031MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Msi2重组蛋白
  • 货号：
    CSB-EP846031MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Msi2重组蛋白
  • 货号：
    CSB-BP846031MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Msi2重组蛋白
  • 货号：
    CSB-MP846031MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Msi2
  • Uniprot No.：
  • 别名：
    Msi2; Msi2h; RNA-binding protein Musashi homolog 2; Musashi-2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-346
  • 氨基酸序列
    MEANGSPGTS GSANDSQHDP GKMFIGGLSW QTSPDSLRDY FSKFGEIREC MVMRDPTTKR SRGFGFVTFA DPASVDKVLG QPHHELDSKT IDPKVAFPRR AQPKMVTRTK KIFVGGLSAN TVVEDVKQYF EQFGKVEDAM LMFDKTTNRH RGFGFVTFEN EDVVEKVCEI HFHEINNKMV ECKKAQPKEV MFPPGTRGRA RGLPYTMDAF MLGMGMLGYP NFVATYGRGY PGFAPSYGYQ FPGFPAAAYG PVAAAAVAAA RGSVLNSYSA QPNFGAPASP AGSNPARPGG FPGANSPGPV ADLYGPASQD SGVGNYISAA SPQPGSGFGH GIAGPLIATA FTNGYH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
  • 基因功能参考文献：
    1. the testis-expressed transcription factor, Tbx1, and the piRNA regulator of gamete development, Piwil1, were both found to be targeted for translational repression by MSI2. PMID: 28884822
    2. Absence of Musashi2 in osteoclast precursors promotes apoptosis and inhibits RANKL-induced NF-kappaB activation, which is essential for osteoclast survival. PMID: 27441652
    3. Msi2 primarily recognizes mRNA 3'UTRs at sites enriched in multiple copies of UAG motifs in epithelial progenitor cells. PMID: 27034466
    4. Msi proteins are dispensable for normal homeostasis and self-renewal of the active intestinal stem cell. PMID: 27799368
    5. our findings reveal the importance of Msi2 in suppressing hair regeneration and maintaining hair follicle stem cells quiescence PMID: 28143780
    6. studies highlight Msi reporters as a unique tool to identify therapy resistance, and define Msi signalling as a central regulator of pancreatic cancer PMID: 27281208
    7. MSI1 and MSI2 display distinct expression profiles during mammalian folliculogenesis and that MSI2 is required for pre-antral follicle development PMID: 26131972
    8. Through expression studies and utilizing a transgenic Msi1 testis-specific overexpression model, we have identified 2 unique RNA-binding targets of MSI1 in spermatogonia, Msi2 and Erh, and have demonstrated a role for MSI1 in translational regulation. PMID: 25782991
    9. Msi proteins contribute to an epithelial gene expression program in neural and mammary cell types. PMID: 25380226
    10. Msi2 is an important regulator of the hematopoietic stem cell (HSC) translatome and balances HSC homeostasis and lineage bias. PMID: 24395885
    11. Ectopic expression of Musashi 2 isoform 1, but not isoform 2, enhanced the cloning efficiency of embryonic stem cells, but both isoforms were required for the self-renewal of embryonic stem cells. PMID: 22496868
    12. Our data indicate that Msi2 maintains the stem cell compartment mainly by regulating the proliferation of primitive progenitors downstream of long term hematopoietic stem cells PMID: 21613258
    13. Pard6a, Prkcz, and Msi2 shRNA-mediated depletion significantly impaired hematopoietic stem cell repopulation. PMID: 20621054
    14. data show that the Musashi-Numb pathway can control the differentiation of chronic myeloid leukemia cells PMID: 20639863
    15. Expression levels in human myeloid leukemia directly correlate with decreased survival in patients, defining MSI2 as a new prognostic marker and new target for therapy. PMID: 20616797
    16. data suggest that msi1 and msi2 are cooperatively involved in the proliferation and maintenance of CNS stem cell populations PMID: 12407178
    17. the complementary expression patterns of the murine Msi paralogues Msi1 and Msi2 during spermatogenesis support the idea of distinct, evolutionarily conserved roles of Msi PMID: 16717192

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  • 亚细胞定位：
    Cytoplasm. Note=Associated with polysomes.
  • 蛋白家族：
    Musashi family
  • 组织特异性：
    Ubiquitous. Expressed in proliferating neural precursor cells.
  • 数据库链接：

    KEGG: mmu:76626

    STRING: 10090.ENSMUSP00000090470

    UniGene: Mm.400451