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  4. Recombinant Mouse RNA-binding protein Musashi homolog 1 (Msi1)

Recombinant Mouse RNA-binding protein Musashi homolog 1 (Msi1)

  • 中文名称：
    小鼠Msi1重组蛋白
  • 货号：
    CSB-YP736820MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Msi1重组蛋白
  • 货号：
    CSB-EP736820MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Msi1重组蛋白
  • 货号：
    CSB-EP736820MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Msi1重组蛋白
  • 货号：
    CSB-BP736820MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Msi1重组蛋白
  • 货号：
    CSB-MP736820MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Msi1
  • Uniprot No.：
  • 别名：
    Msi1; Msi1h; RNA-binding protein Musashi homolog 1; Musashi-1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-362
  • 氨基酸序列
    METDAPQPGL ASPDSPHDPC KMFIGGLSWQ TTQEGLREYF GQFGEVKECL VMRDPLTKRS RGFGFVTFMD QAGVDKVLAQ SRHELDSKTI DPKVAFPRRA QPKMVTRTKK IFVGGLSVNT TVEDVKHYFE QFGKVDDAML MFDKTTNRHR GFGFVTFESE DIVEKVCEIH FHEINNKMVE CKKAQPKEVM SPTGSARGRS RVMPYGMDAF MLGIGMLGYP GFQATTYASR SYTGLAPGYT YQFPEFRVER SPLPSAPVLP ELTAIPLTAY GPMAAAAAAA AVVRGTGSHP WTMAPPPGST PSRTGGFLGT TSPGPMAELY GAANQDSGVS SYISAASPAP STGFGHSLGG PLIATAFTNG YH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
  • 基因功能参考文献：
    1. Msi1 has two RNA-binding domains. PMID: 28753936
    2. leptin's direct stimulatory actions on gonadotrope GnRHR correlate with a direct inhibition of expression of the posttranscriptional regulator MSI1. There also is a direct MSI1 interaction with 3'-UTR of Gnrhr mRNA. PMID: 29228137
    3. Msi proteins are dispensable for normal homeostasis and self-renewal of the active intestinal stem cell. PMID: 27799368
    4. photoreceptors lack prototypical neuronal splicing factors and their splicing profile is driven to a significant degree by the Musashi 1 (MSI1) protein. A striking feature of the photoreceptor splicing program are exons that display a "switch-like" pattern of high inclusion levels in photoreceptors and near complete exclusion outside of the retina. PMID: 27541351
    5. Given that deregulated fatty acid metabolism plays a key role in kidney fibrosis, these results demonstrate a novel connection between fatty acid and Msi1, an RNA-binding protein, in kidney fibrosis PMID: 27129280
    6. A Mouse Model of Targeted Musashi1 Expression in Whole Intestinal Epithelium Suggests Regulatory Roles in Cell Cycle and Stemness. PMID: 26303183
    7. studies highlight Msi reporters as a unique tool to identify therapy resistance, and define Msi signalling as a central regulator of pancreatic cancer PMID: 27281208
    8. the RNA-binding protein Musashi 1 competes with miR130a and -206 for interaction with tachykinin mRNA PMID: 26373800
    9. MSI1 and MSI2 display distinct expression profiles during mammalian folliculogenesis and that MSI2 is required for pre-antral follicle development PMID: 26131972
    10. Osteoarticular expression of Msi1 protein is increased in joints with CIA-induced lesion and absent in nonlesioned joints, suggesting that this protein is expressed when the lesion is produced in order to favor tissue repair. PMID: 26064941
    11. Msi1 is critical for constructing functional blood-testis barrier &maintaining spermatogenesis. Msi1 regulates Sertoli cell fate following heat-induced injury, likely through induction of stress granule formation & activation of p-ERK1/2 signaling PMID: 25717188
    12. Through expression studies and utilizing a transgenic Msi1 testis-specific overexpression model, we have identified 2 unique RNA-binding targets of MSI1 in spermatogonia, Msi2 and Erh, and have demonstrated a role for MSI1 in translational regulation. PMID: 25782991
    13. Msi proteins contribute to an epithelial gene expression program in neural and mammary cell types. PMID: 25380226
    14. MSI1 is specifically inhibited by 18-22 carbon omega-9 monounsaturated fatty acids; MSI1 regulates stearoyl-CoA desaturase PMID: 24935936
    15. This study suggests that ciliogenic Rfx transcription factors regulate Msi1 expression in neural stem/progenitor cells. PMID: 25058468
    16. Musashi1 binds to the Musashi binding element and that this element is required for polyadenylation in oocytes. PMID: 24586322
    17. Msi1-dependent post-transcriptional enhancement of m-Numb is crucial in gastric epithelial regeneration. PMID: 23308249
    18. RNA-binding protein Musashi1 modulates glioma cell growth through the post-transcriptional regulation of Notch and PI3 kinase/Akt signaling pathways. PMID: 22428049
    19. data show that ectopic MSI1 expression may contribute to tumorigenesis in selected bladder cancers through multiple mechanisms and reveal a previously unrecognized function of Musashi1 in the regulation of SG formation PMID: 20477901
    20. A regulatory element for Msi1 transcription in neural stem/progenitor cells is located in the sixth intron of the Msi1 gene. PMID: 21486496
    21. These data provide evidence that nuclear, and cytoplasmic sequestering of Musashi-1 in retinal cells is context-specific, and may contribute to downstream functioning of Musashi-1. PMID: 21320487
    22. Musash1 and Hes1 high expression cells locate predominantly in impaired small-intestinal epithelium after transplantation and contribute to epithelial regeneration. PMID: 21221806
    23. Musashi1 cooperates in abnormal cell lineage protein 28 (Lin28)-mediated let-7 family microRNA biogenesis in early neural differentiation. PMID: 21378162
    24. overexpression induces tumorigenesis through Wnt and Notch activation PMID: 20826465
    25. The Msi1-positive cells can differentiate into neural and intestinal epithelial-like cells in vivo. PMID: 20670215
    26. Msi1-mediated posttranscriptional regulation of Robo3 controls midline crossing of precerebellar neurons. PMID: 20696379
    27. Positional specification in a neural stem cell line gene expression is modulated by the RNA-binding protein Musashi1 PMID: 19645571
    28. Msi1-expression is induced after brain ischemia and may be involved in the reactivation of astrocytes, including their proliferation. PMID: 20036290
    29. Expression of the Msi1 and Hes1 small intestinal stem cell markers in the small intestine was not homogenous and the markers were more highly expressed in jejunal tissues. PMID: 20110912
    30. Musashi-1 is present in a few epithelial cells just above the Paneth cells in the small intestinal crypt; Musashi-1 and Hes1 were coexpressed in the crypt base columnar cells located between the Paneth cells PMID: 12560091
    31. Distribution of Musashi-1-positive cells corresponded with that of stem cells, as outlined in previous reports, implying that Musashi-1 is a key control element of asymmetrical division within the colon crypt. PMID: 12924647
    32. Msi1 immunostaining was found in a variety of supporting cells but not in outer hair cells in the organ of Corti. Although an immunoreactive ring was found around the inner hair cells, it also seemed to originate from the supporting cells. PMID: 14700731
    33. By immunohistochemistry and RT-PCR, researchers demonstrate for the first time the expression of Musashi1 during development of the inner ear. PMID: 15076722
    34. Important for digestive organ development, particularly for epithelial differentiation. PMID: 16287482
    35. It is proposed that ELAV RNA-binding proteins exert an important post-transcriptional control on Msi1 expression in the transition from proliferation to neural differentiation of stem/progenitor cells. PMID: 16554442
    36. the complementary expression patterns of the murine Msi paralogues Msi1 and Msi2 during spermatogenesis support the idea of distinct, evolutionarily conserved roles of Msi PMID: 16717192
    37. The RNA-binding protein, Msi1, is produced in mouse eyes from embryonic stages until adulthood. PMID: 17768378
    38. Msi1 regulates oligodendrocyte lineage cell differentiation and survival. PMID: 18098125
    39. These results identify Msi1 as a key determinant of the mammary lineage through its ability to coordinate cell cycle entry and activate the Notch and Wnt pathways by a novel autocrine process involving PLF1 and DKK3. PMID: 18362162
    40. Msi-1 involved in cancer cell proliferation, inhibition of apoptosis, and mitotic catastrophe, suggesting an important potential mechanism for its role in tumorigenesis. PMID: 18471519
    41. Poly(A) binding protein (PABP) is identified as an Msi1-binding protein, and Msi1 competes with eIF4G for PABP binding. PMID: 18490513
    42. Data show that doublecortin (dcx) mRNA is a specific binding target of Musashi1 in vitro. PMID: 19573529

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Musashi family
  • 组织特异性：
    Expressed in neural stem and progenitor cells (at protein level). Detected in olfactory bulb, brain stem, small intestine, and at low levels in brain cortex, hippocampus and ovary. Detected in neural progenitor cells, including neural stem cells.
  • 数据库链接：

    KEGG: mmu:17690

    STRING: 10090.ENSMUSP00000120516

    UniGene: Mm.391995