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  4. Recombinant Mouse RAC-gamma serine/threonine-protein kinase (Akt3)

Recombinant Mouse RAC-gamma serine/threonine-protein kinase (Akt3)

  • 货号：
    CSB-YP894361MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP894361MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP894361MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP894361MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP894361MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Akt3RAC-gamma serine/threonine-protein kinase; EC 2.7.11.1; Protein kinase Akt-3; Protein kinase B gamma; PKB gamma; RAC-PK-gamma
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-479
  • 氨基酸序列
    SDVTIVKEG WVQKRGEYIK NWRPRYFLLK TDGSFIGYKE KPQDVDLPYP LNNFSVAKCQ LMKTERPKPN TFIIRCLQWT TVIERTFHVD TPEEREEWTE AIQAVADRLQ RQEEERMNCS PTSQIDNIGE EEMDASTTHH KRKTMNDFDY LKLLGKGTFG KVILVREKAS GKYYAMKILK KEVIIAKDEV AHTLTESRVL KNTRHPFLTS LKYSFQTKDR LCFVMEYVNG GELFFHLSRE RVFSEDRTRF YGAEIVSALD YLHSGKIVYR DLKLENLMLD KDGHIKITDF GLCKEGITDA ATMKTFCGTP EYLAPEVLED NDYGRAVDWW GLGVVMYEMM CGRLPFYNQD HEKLFELILM EDIKFPRTLS SDAKSLLSGL LIKDPNKRLG GGPDDAKEIM RHSFFSGVNW QDVYDKKLVP PFKPQVTSET DTRYFDEEFT AQTITITPPE KYDDDGMDGM DNERRPHFPQ FSYSASGRE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis.
  • 基因功能参考文献：
    1. The s find in Mus musculus, each AKT isoform has a unique expression pattern in the hippocampus. AKT1, but not AKT2 or AKT3, is required for late long term potetiation (LTD) through regulating activity-induced protein synthesis. Interestingly, AKT activity inhibits mGluR-LTD, with overlapping functions for AKT1 and AKT3. PMID: 29173281
    2. Behavioral data on Akt3-/- and Akt1-/- mice demonstrated that Akt3 but not Akt1 is required for spatial learning. Histological analyses on synapse, dendrite, and myelin markers suggested that Akt3 but not Akt1 is important for the white matter integrity, and that Akt single isoform does not play a pivotal role on neuronal survival. PMID: 27671373
    3. Results show that AKT3 influences outcome of pneumococcal meningitis in human patients; validated the findings in a mouse experimental pneumococcal meningitis model. PMID: 27193124
    4. findings provide novel insight into the neurodevelopmental role of Akt3, identify a non-redundant role for Akt3 in the development of prefrontal cortical-mediated cognitive function and show that Akt3 is potentially the dominant regulator of AKT/mTOR signaling in brain PMID: 28467426
    5. miR-15b-5p is a critical regulator of human EC proliferation and migration by targeting the AKT3 pathway. PMID: 28254819
    6. The predominant AKT isoform in the central nervous system, AKT3, induces much more robust axon regeneration than AKT1 and that activation of mTORC1 and inhibition of GSK3beta are two critical parallel pathways for AKT-induced axon regeneration. PMID: 27026523
    7. Downregulation of AKT3 increases migration and metastasis in triple negative breast cancer cells by upregulating S100A4. PMID: 26741489
    8. In viable Akt three-isoforms conditional knockout mice, total Akt levels were reduced in the adult brain. They had increased levels of phosphorylated tau, GSK3alpha and PKA substrates. No significant changes in p-tau levels were found in Akt3-/-)mice. PMID: 26227811
    9. untreated Akt1 and Akt2/Akt3 double knockout mice display significant hearing loss, indicating a role for these isoforms in normal hearing. PMID: 25811375
    10. MicroRNA-207 enhances radiation-induced apoptosis by targeting Akt3 in cochlea hair cells. PMID: 25275594
    11. Blocking Akt1 or Akt3 but not Akt2 expression prohibits cell proliferation and reprogramming. PMID: 25037569
    12. Mechanistic investigations uncovered opposing functions for different Akt isoforms in this regulation, where Akt1 promotes and Akt3 inhibits vascular tumor growth. PMID: 25388284
    13. MiR-29 modulates growth and promotes differentiation of skeletal muscle through the post-transcriptional downregulation of Akt3. PMID: 23764849
    14. AKT3 controls mitochondrial biogenesis and autophagy via regulation of the major nuclear export protein CRM-1. PMID: 24081905
    15. Findings demonstrate that N-cadherin suppresses Akt3 to promote cell motility and highlight the intricate regulation of Akt isoforms by N-cadherin during metastasis. PMID: 22410780
    16. AKT3-deficient mice have a more severe disease course than wild-type mice during experimental autoimmune encephalomyelitis (EAE), because AKT3 is necessary for proper central nervous system (CNS) cell integrity. PMID: 23335751
    17. Results demonstrate that integrin outside-in signaling and platelet spreading requires Src family kinase-dependent and ADP receptor-amplified activation of the PI3K-Akt-GSK-3beta pathway. PMID: 22814751
    18. nonredundant atheroprotective role for Akt3 exerted via the previously unknown link between the Akt signaling pathway and cholesterol metabolism. PMID: 22632897
    19. These findings suggest an important role for Akt3 in the regulation of RCAS1 and VEGF secretion in ovarian cancer cells. PMID: 21351097
    20. Akt3 plays an important and distinct role in platelet activation and in thrombosis. PMID: 21821713
    21. Akt3 missense mutation is associated with enhanced kinase activity and seizure susceptibility. PMID: 21159799
    22. Akt3-deficient brains are reduced in size. PMID: 15713641
    23. Study using Pkb gamma-deficient mice suggests a crucial role for Pkb gamma in postnatal brain development. PMID: 15930105
    24. Akt1 gene is more essential than Akt3 for embryonic development and survival PMID: 16287854
    25. Results demonstrate an in vivo role for both Akt2 and Akt3 in the determination of whole animal size and individual organ sizes. PMID: 16923958
    26. There is not a sex difference in the degree of Met5-enkephalin induced cardioprotection, however males use PI3K/Akt1/Akt2 pathway and females use PI3K/Akt3 pathway. PMID: 17982014
    27. Akt3 silencing results in cytoplasmic accumulation of the master regulator of mitochondrial biogenesis, PGC-1alpha, & reduction in known PGC-1alpha target genes. subtle abnormal mitochondrial phenotype is observed in brain tissue of AKT3 knockout mice. PMID: 18524868
    28. Akt1/2/3 have different roles in mammary gland differentiation during pregnancy and this may reflect differences in hormonal signaling. PMID: 18561256

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated after cell stimulation leading to its translocation.
  • 蛋白家族：
    Protein kinase superfamily, AGC Ser/Thr protein kinase family, RAC subfamily
  • 组织特异性：
    Isoform 1 is expressed in prostate, testis, uterus and mammary gland and isoform 2 is expressed in prostate, testis and mammary gland.
  • 数据库链接：

    KEGG: mmu:23797

    STRING: 10090.ENSMUSP00000106789

    UniGene: Mm.235194