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  4. Recombinant Mouse Putative myosin light chain kinase 3 (Mylk3)

Recombinant Mouse Putative myosin light chain kinase 3 (Mylk3)

  • 中文名称：
    小鼠Mylk3重组蛋白
  • 货号：
    CSB-YP659731MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Mylk3重组蛋白
  • 货号：
    CSB-EP659731MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Mylk3重组蛋白
  • 货号：
    CSB-EP659731MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Mylk3重组蛋白
  • 货号：
    CSB-BP659731MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Mylk3重组蛋白
  • 货号：
    CSB-MP659731MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Mylk3; Myosin light chain kinase 3; EC 2.7.11.18; Cardiac-MyBP-C-associated Ca/CaM kinase; Cardiac-MLCK
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-795
  • 氨基酸序列
    MSGVSEEDPE GLAPQGLPAL GGACLATMDK KLNVLTEKVD RLLHFQEDVT EKLQCVCQGM DHLEQDLHRL EASRELSLAG SGSTPPTTAQ AAWPEVLELV RAVRQEGAQH GARLEALFKM VVAVDRAITL VGSTFQNSKV ADFIMQGTVP GRKGSLADGP EENKEQAEVA GVKPNHVLTT GGVQADASRT LWEESQKEDI PVRTVEGLPL IINTSLKGAD LTQAGASLRQ GVEVLGPGQV PLPTEAESRL PETASENTGA TLELSVAIDR ISEVLTSLKM SQGGGQETSS SKPDCWLSEE AMRLSSGPLP QPLGPLTPDS DIHSGDALPR IPINMQEMAT PGELLETQSG SPIGSAEAPG LGTVLEDQIP KGARPFPPLP KRSSNNGGMS AEEEIGSGAE PMRGPSLATR DWRDETVGTT DLQQGIDPGA VSPEPGKDHA AQGPGRTEAG RLSSAAEAAI VVLDDSAAPP APFEHRVVSI KDTLISAGYT VSQHEVLGGG RFGQVHRCTE RSTGLALAAK IIKVKNVKDR EDVKNEVNIM NQLSHVNLIQ LYDAFESKSS FTLIMEYVDG GELFDRITDE KYHLTELDVV LFTRQICEGV HYLHQHYILH LDLKPENILC VSQTGHQIKI IDFGLARRYK PREKLKVNFG TPEFLAPEVV NYEFVSFPTD MWSVGVITYM LLSGLSPFLG ETDAETMNFI VNCSWDFDAD TFKGLSEEAK DFVSRLLVKE KSCRMSATQC LKHEWLSHLP AKASGSNVRL RSQQLLQKYM AQSKWKKHFH VVTAVNRLRK FPTCP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Kinase that phosphorylates MYL2 in vitro. Has been proposed to be calmodulin-dependent, although MYL2 phosphorylation has also been observed in the presence or absence of calmodulin. Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility.
  • 基因功能参考文献：
    1. Acanthopanax senticosus polysaccharide pretreatment may be associated with inhibition of the NF-kappaB/MLCK pathway and concomitant amelioration of LPS-induced tight junction dysfunction of intestinal epithelium in endotoxemia PMID: 28405145
    2. that MLCK is competing with centrally active ROCK for a limiting pool of monomer with which to drive lamellar filament assembly PMID: 28114272
    3. This study demonstrated that catchlike property during dynamic performance in mouse hindlimb muscles with (wild-type) and without MLCK(-/-) ) the primary mechanism for PTP (myosin phosphorylation). PMID: 26802366
    4. Ablation of cardiac Mylk3 suggests that Mylk3 plays a pivotal role in the transition from compensated to decompensated hypertrophy via cardiac myocyte atrophy and sarcomeric disorganization. PMID: 27025239
    5. Data suggest that galectin-3 in hepatocellular carcinoma cells promotes cell migration by inducing RhoA GTPase activity, activating Mlck, up-regulating phosphorylation of MLC2 (myosin light chain 2), and inducing actin rearrangement. PMID: 26146960
    6. epithelial MLCK-activated brush border fanning by IFN-gamma promotes adherence and internalization of normally noninvasive enteric bacteria PMID: 24911373
    7. MLCK may regulate the structure and the motility of stereocilia through F-actin polymerization. PMID: 24481113
    8. Silencing TNFR2, but not TNFR1, resulted in restoration of epithelial tight junction (TJ) associated with decreased MLCK expression. PMID: 24520376
    9. Calcium regulation of MLCK facilitates GLUT4-mediated glucose uptake in 3T3-L1 adipocytes. PMID: 24116218
    10. nmMLCK has a critical role in thrombin-induced endothelial cell inflammation and lung PMN infiltration PMID: 23555849
    11. these studies show that the IL-1beta-induced increase in intestinal tight junction permeability was regulated by p38 kinase activation of ATF-2 and by ATF-2 regulation of MLCK gene activity PMID: 23656735
    12. analysis of differential contributions of MLCK to signaling in smooth muscles PMID: 23362260
    13. MLCK-dependent MLC phosphorylation mediates intestinal epithelial barrier dysfunction after severe burn injury. PMID: 22529961
    14. Nonmuscle myosin light-chain kinase contributes to atherosclerosis by regulating endothelial barrier function and monocyte migration via mechanisms involving not only kinase-mediated MLC phosphorylation but also Src activation. PMID: 21670231
    15. guanylyl cyclase C signaling plays a protective role in the integrity of the intestinal mucosal barrier by regulating myosin light chain kinase activation and tight junction disassembly. PMID: 21305056
    16. cMLCK appears to be the predominant protein kinase that maintains basal RLC phosphorylation that is required for normal physiological cardiac performance in vivo PMID: 20943660
    17. Ubiquitously expressed smooth muscle MLCK activity in MLCK null mice may be sufficient, but is not necessary for cytokinesis and early morphogenesis; other Ca2+-dependent kinases can phosphorylate myosin light-chain (20) and induce contraction in embryos. PMID: 15467390
    18. MLCK is involved in egg polarity because inhibitors prevent sperm chromatin-induced cortical reorganization. PMID: 15680356
    19. Inhibiting MLCK increases the number of apoptotic cells and retards the growth of mammary cancer cells in mice. PMID: 15988034
    20. Myosin light chain phosphorylation by the dedicated skeletal muscle Ca(2+)/calmodulin-dependent MLCK is a primary biochemical mechanism for tension potentiation due to repetitive stimulation in fast-twitch skeletal muscle. PMID: 16299103
    21. MLCK may be a regulatory target of Ca(2+)/calmodulin and may play an important role in insulin-stimulated glucose transport in adipocytes. PMID: 16672772
    22. MLCK is involved in the activation process of nonselective cationic currents by muscarinic stimulation in murine ileal myocytes PMID: 18066127
    23. characterization of cardiac MKLCK; both overexpression and knockdown of cardiac MLCK in cultured cardiomyocytes revealed that cardiac MLCK is likely a new regulator of MLC2 phosphorylation, sarcomere organization, and cardiomyocyte contraction PMID: 18202317

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Protein kinase superfamily, CAMK Ser/Thr protein kinase family
  • 组织特异性：
    Restricted to cardiomyocytes (at protein level). Down-regulated in heart after experimental myocardial infarction at the protein level; no significant changes at the mRNA level.
  • 数据库链接：

    KEGG: mmu:213435

    STRING: 10090.ENSMUSP00000034133

    UniGene: Mm.32804