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  4. Recombinant Mouse Protein sprouty homolog 2 (Spry2)

Recombinant Mouse Protein sprouty homolog 2 (Spry2)

  • 中文名称：
    小鼠Spry2重组蛋白
  • 货号：
    CSB-YP886172MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Spry2重组蛋白
  • 货号：
    CSB-EP886172MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Spry2重组蛋白
  • 货号：
    CSB-EP886172MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Spry2重组蛋白
  • 货号：
    CSB-BP886172MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Spry2重组蛋白
  • 货号：
    CSB-MP886172MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Spry2
  • Uniprot No.：
  • 别名：
    Spry2; Protein sprouty homolog 2; Spry-2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-315
  • 氨基酸序列
    MEARAQSGNG SQPLLQTAHD SGRQRGEPDP RDALTQQVHV LSLDQIRAIR NTNEYTEGPT VVPRPGLKPA PRPSTQHKHE RLHGLPEHRQ PPRLQPSQVH SSRAPLSRSI STVSSGSRSS TRTSTSSSSS EQRLLGPSFS HGPAAADGII RVQPKSELKP GDVKPLSKDD LGLHAYRCED CGKCKCKECT YPRPLPSDWI CDKQCLCSAQ NVIDYGTCVC CVKGLFYHCS NDDEDNCADN PCSCSQSHCC TRWSAMGVMS LFLPCLWCYL PAKGCLKLCQ GCYDRVNRPG CRCKNSNTVC CKVPTVPPRN FEKPT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

We want to order CSB-BP886172MO, 0.05mg. However, we want the tag type to be biotin tag.
Can you provide this protein in biotin tag?

A:
Very nice to receive your inquiry.
(1)If you the Biotin label, we can provide the biotinylated service, It's labeled in vitro by random.
It's labeled at the free amino terminal of all lysine (primaquine) not only at the N-terminus, which means the amino acids containing the lysine residue can all be labelled. There will be extra charges for biotinylated service. Lead time will be extended for 3 working days.
(2)If you need biotin tag, we can provide N-terminal 6xHis-Avi-tagged or N-terminal 6xHis-tagged and C-terminal Avi-tagged,
As the specific biotin tagged protein has the risk of not being expressed, so if it's failed to express, we need to charge some cost and we will provide the constructed plasmid.
You can perform biotinylation in vitro based on either of these two tags.
Avi-tag itself can be biotinylated in vitro and in vivo.
In vivo, one method is to use a specific strain: https://www.avidity.com/avitag/avb-101-glycerol-stock-0
The other method is to build a co-expression, which is relatively difficult.
So these two kinds of in vivo biotinylated are not recommended.
What we express is non-biotinylated protein. If customer wants biotinylation, the customer can purchase enzyme or kit for biotinylation in vitro. We do not provide this in vitro biotinylation labeling service.
You can refer to this link: https://www.avidity.com/avitag/bira-500-bira-biotin-protein-ligase-standard-reaction-kit

靶点详情

  • 功能：
    Antagonist of fibroblast growth factor (FGF) pathways via inhibition of FGF-mediated phosphorylation of ERK1/2. Thereby acts as an antagonist of FGF-induced retinal lens fiber differentiation, may inhibit limb bud outgrowth and may negatively modulate respiratory organogenesis. Inhibits TGFB-induced epithelial-to-mesenchymal transition in retinal lens epithelial cells. Inhibits CBL/C-CBL-mediated EGFR ubiquitination.
  • 基因功能参考文献：
    1. These results identify Spry2 as a critical regulator of endochondral bone formation that modulates signaling in both osteoblast and chondrocyte lineages. PMID: 27130872
    2. MYB acts on MAPK signaling by directly regulating transcription of the gene encoding the negative modulator SPRY2. PMID: 27748374
    3. Spry2 is a novel unfolded protein response target and its upregulation is dependent on PERK. Knockdown of Spry2 resulted in reduced expression of Serca2, reduced endoplasmic reticulum calcium levels, and induction of the UPR. Spry2 deletion in the adult mouse beta-cell caused hyperglycemia and hypoinsulinemia. PMID: 28246293
    4. these results establish SPRY2 as a critical negative regulator of BCR-mediated MAPK-Erk signaling in chronic lymphocytic leukemia , thereby providing one of the molecular mechanisms to explain the clinical heterogeneity of chronic lymphocytic leukemia. PMID: 26809508
    5. In the present study, it is demonstrated that Spry2 and -4 participate in KA induced neurodegeneration possibly through inhibition of ERK signaling. PMID: 26540287
    6. Study revealed that suppression of Spry2 expression induced proliferation and differentiation of osteoblastic cells upon bFGF and EGF stimulation, whereas it diminished proliferation of gingival epithelial cells. PMID: 25399781
    7. The data showed enhanced axon outgrowth and improved long-distance axon regeneration in sprouty2 deficient mice PMID: 25104556
    8. Sprouty2 acts as an inhibitor of CrkL-Rap1 signaling. PMID: 20136656
    9. we reveal that SPRY2 expression is regulated by FOXO3a and beta-catenin nuclear activity in colon cancer PMID: 23624922
    10. We propose that Sprouty genes(Spry2 and Spry4) were implicated during evolution in reduction of the cheek teeth in Muridae, and their deletion can reveal ancestral stages of murine dental evolution. PMID: 23606267
    11. study thus reveals a novel Spry2 function, which mediates endothelial contact inhibition and barrier integrity PMID: 23232625
    12. Sprouty2 signaling inhibits Akt activation and branch initiation in the developing mammary epithelium. PMID: 23665174
    13. Inactivation of Spry2 accelerates AKT-induced hepatocarcinogenesis via activation of Ras/mitogen-activated protein kinase and pyruvate kinase M2 pathways. PMID: 22617155
    14. Nuclear FGF8 could function as transcriptional regulator to induce Sprouty2 in the isthmus. PMID: 22404534
    15. our results imply that Sprouty2 and -4 are downregulated in the hippocampus during postnatal brain development and that they can act as regulators of developmental axon growth. PMID: 21240919
    16. G Protein-regulated inducer of neurite outgrowth (GRIN) modulates Sprouty protein repression of mitogen-activated protein kinase (MAPK) activation by growth factor stimulation PMID: 22383529
    17. ITSN1 binds Pro-rich regions in both Cbl and Spry2, and interaction of ITSN1 with Spry2 disrupts Spry2-Cbl interaction, resulting in enhanced ubiquitylation of the EGFR. PMID: 22158968
    18. Interestingly, changes in sprouty gene dosage led to a graded change in incisor number, with progressive decreases in sprouty dosage leading to increasing numbers of teeth. PMID: 21862563
    19. These results indicate that endogenous Spry2 functions to downregulate angiogenesis in the healing murine skin wound PMID: 21076020
    20. These results suggest that Sprouty2 regulates palate mesenchymal cell proliferation via FGF signaling and is involved in palatal shelf elevation. PMID: 21195053
    21. Spry2 regulates downstream signaling mediated by glial cell line-derived neurotrophic factor /RET tyrosine kinase signaling complex in vivo PMID: 21070764
    22. Studies indicate that three genes within the same GDNF-RET signaling pathway have been implicated in achalasia: GDNF, RET, and SPRY2. PMID: 20700745
    23. Spry2 does not directly regulate Raf-1 kinase activity, but instead acts as a scaffolding protein that assists interactions between Raf-1 kinase and its direct regulators PMID: 20356462
    24. Data suggest that the dynamic regulation of Sprouty by Shp2 might be important not only for modulating Kras signaling in lens and lacrimal gland development, but also for RTK signaling in general. PMID: 20215346
    25. Results imply that Sprouty2 is highly expressed in adult peripheral neurons and its down-regulation strongly promotes elongative axon growth by activation of the Ras/Raf/ERK pathway. PMID: 19683577
    26. Fgf8, Spry2 and Sef belong to a synexpression group and may functionally interact during mouse embryonic development PMID: 11960706
    27. mSprouty2 inhibits FGF10-activated MAP kinase by differentially binding to upstream target proteins. PMID: 12225946
    28. Spry1 and Spry2 function in a negative feedback mechanism in which the inhibitor activity is controlled rapidly and reversibly by post-translational mechanisms PMID: 12402043
    29. Tyr55 is a key residue for Sprouty2 phosphorylation and its phosphorylation is required for Sprouty2 to inhibit RTK signaling PMID: 15004239
    30. Sprouty proteins regulate ureteric branching by coordinating reciprocal epithelial Wnt11, mesenchymal Gdnf and stromal Fgf7 signalling during kidney development PMID: 15201220
    31. Sef and Sproutys function synergistically to regulate Gbx2 expression in the anterior hindbrain. PMID: 15729686
    32. Results provide evidence that antagonism of fibroblast growth factor signaling by Sprouty2 is essential for establishing the cytoarchitecture of the organ of Corti and for hearing. PMID: 15809037
    33. We show Sprouty2 to be a negative regulator of GDNF for the neonatal development or survival of enteric nerve cells. PMID: 15937482
    34. Sprouty2 protein contains an iron-sulfur complex that can exist in an oxidized, reduced, or nitrosylated state and purified it assembles into large monodisperse spheres containing approximately 24 polypeptides per particle. PMID: 16172380
    35. SPRY2 mediates its anti-proliferative actions by altering PTEN content and activity PMID: 16371366
    36. the Sprouty proteins are involved normally in mediating the sexually dimorphic signaling of FGF9 and controlling cell migration from the mesonephros during testis development PMID: 16675530
    37. suggest both redundant and non-redundant functions for Sprouty2 and Sprouty4 on embryonic development and FGF signaling PMID: 17156747
    38. in the context of K-rasG12D-mediated lung tumorigenesis, Sprouty-2 is also up-regulated and functions as a tumor suppressor to limit tumor number and overall tumor burden PMID: 17369402
    39. Spry2 is involved in the development of the CNS by inhibiting both neuronal differentiation and survival through a negative-feedback loop that downregulates neurotrophic factors-driven signaling pathways. PMID: 17599098
    40. Mice carrying a deletion that removes the fibroblast growth factor signaling antagonist Spry2 have cleft palates; cell proliferation is excessive and accompanied by abnormal progression of shape changes and movements. PMID: 17693063
    41. By selectively modulating the downstream effects of Spry2, Tesk1 may thus serve as a molecular determinant of the signaling outcome. PMID: 17974561
    42. Rescue experiments supported the idea of a specific relationship between FGF6 and Spry 2, both being known for their particular involvement in myogenesis. PMID: 18049951
    43. Internal pressure regulates the FGF10-FGFR2b-Sprouty2 pathway and thus the speed of epithelial branching of embryonic mouse lung. PMID: 18082381
    44. an increase in miR-21 enhances the formation of various types of cellular protrusions through directly targeting and down-regulating SPRY2 PMID: 18508928
    45. These results demonstrate that Sprouty 2 overexpression inhibited both lung tumor initiation and subsequent tumor growth. PMID: 18635814
    46. The results suggest that DYRK1A positively regulates FGF-mitogen-activated protein kinase signaling by phosphorylation-dependent impairment of the inhibitory activity of Sprouty2. PMID: 18678649
    47. This study is the first that reported the association of Spry2 with TNF-alpha signaling pathway. PMID: 18713620
    48. inhibiting Siah2 activity by means of a dominant-negative Siah2 RING mutant primarily reduced tumorigenesis through the action of Sprouty 2, a negative regulator of Ras signaling PMID: 18946040
    49. increased levels of FGF signaling in Spry2(-/-) embryos led to significantly decreased apoptosis and increased proliferation in the R(2) bud PMID: 19127536
    50. confluent p38alpha-deficient cells fail to downregulate Spry2, providing them in turn with sustained EGFR signaling that facilitates cell overgrowth and oncogenic transformation PMID: 19364817

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Cell projection, ruffle membrane.
  • 蛋白家族：
    Sprouty family
  • 组织特异性：
    Expressed in the testes and brain (at protein level). In adult, highly expressed in the lung, heart and at lower levels in skeletal muscle and kidney.
  • 数据库链接：

    KEGG: mmu:24064

    STRING: 10090.ENSMUSP00000022709

    UniGene: Mm.489870