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  4. Recombinant Mouse Protein phosphatase 1 regulatory subunit 1B (Ppp1r1b)

Recombinant Mouse Protein phosphatase 1 regulatory subunit 1B (Ppp1r1b)

  • 货号：
    CSB-YP720180MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP720180MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP720180MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP720180MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP720180MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ppp1r1b; Protein phosphatase 1 regulatory subunit 1B; DARPP-32; Dopamine- and cAMP-regulated neuronal phosphoprotein
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-194
  • 氨基酸序列
    MDPKDRKKIQ FSVPAPPSQL DPRQVEMIRR RRPTPAMLFR VSEHSSPEEE ASPHQRTSGE GHHPKSKRPN PCAYTPPSLK AVQHLQTISN LSENQASEEE DELGELRELG YPQEDDEEDE DEEEDEEEDS QAEVLKGSRG TVGQKPTCGR GLEGPWERPP PLDEPQRDGN SEDQVEGRAT LSEPGEEPQH PSPP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Inhibitor of protein-phosphatase 1.
  • 基因功能参考文献：
    1. This study strongly suggests that inflammation-regulated DARPP-32 constitutes a key component of H. pylori-mediated gastric tumourigenesis. PMID: 27590997
    2. the results suggest that by inducing dephosphorylation of DARPP-32 at Ser-97 and altering its cytonuclear distribution, glutamate may counteract dopamine/D1 receptor/PKA signaling at multiple cellular levels. PMID: 27998980
    3. Darpp32 interaction with beta-adducin may be the molecular pathway by which environmental changes may rapidly alter responsiveness of striatal neurons involved in the reward system. PMID: 26639316
    4. Results show that Darpp-32 was expressed in normal mouse mammary tissue and in some breast tumors, whereas t-Darpp was found exclusively in mammry tumors. PMID: 25128420
    5. analysis of cAMP-activated DARPP-32 signalling to the nucleus PMID: 25692798
    6. Results describe the development of new vascular mouse model with a conditional knockout allele of Tsc1 with a Darpp32-Cre allele. This mouse displayed accelerated formation of both kidney cystadenomas and paw hemangiosarcomas. PMID: 25548102
    7. Cannabinoid-stimulated G-protein activity did not differ between DARPP-32 KO and WT mice treated with vehicle or repeated THC. PMID: 26099530
    8. In this work, the dissected postsynaptic topology of the DARPP-32 phosphoprotein provides strong evidence for a compartmentalized and confined distribution in dendritic spines. PMID: 24058659
    9. Adenosine AA receptors in striatal glutamatergic terminals and GABAergic neurons oppositely modulate psychostimulant action and DARPP-32 phosphorylation. PMID: 24312250
    10. This study demonistrated that environment enhanced Darpp32 expression in brain and enhanced totaroad perfermance and short term working menory. PMID: 23558143
    11. DARPP32 has a role in pluripotent stem cell differentiation toward medium-sized spiny neurons PMID: 23250204
    12. l-DOPA-induced activation of ERK and mTORC1 requires DARPP-32 and indicates the importance of the cAMP/DARPP-32 signaling cascade in dyskinesia. PMID: 22753408
    13. DARPP-32 increases interactions between epidermal growth factor receptor and ERBB3 to promote tumor resistance to gefitinib. PMID: 21741919
    14. Data show that the loss of DARPP-32 in striatopallidal neurons produced a robust increase in locomotor activity. PMID: 20682746
    15. results indicate that under chronic dopamine deficiency (a) the A(2A)/cAMP/PKA/DARPP-32 cascade is overactive due to the elevated endogenous phospho-DARPP-32 levels and (b) the A(2A) receptor modulatory effect on ERK1/2 signaling is dysregulated PMID: 19852993
    16. Our data demonstrate that beta(1)- and alpha(2)-adrenoceptors regulate DARPP-32 phosphorylation in neostriatal neurons. PMID: 20236221
    17. Data confirmed that CR specifically increased dopamine- and cAMP-regulated phosphoprotein (Darpp-32), a key regulator of dopamine receptor signaling, in the amygdala. PMID: 19737990
    18. DARPP-32 is essential not only for dopaminergic but also for serotonergic neurotransmission. PMID: 11880652
    19. stimulatory effect of caffeine on motor activity in mice was greatly reduced following genetic deletion of DARPP-32 (dopamine- and cyclic AMP-regulated phosphoprotein of relative molecular mass 32,000) PMID: 12181566
    20. adenosine A(2A)DARPP-32 signaling is stimulated by mGlu receptors by stimulating the adenosine A(2A) receptor-mediated formation of cAMP in an extracellular signal-regulated kinase-dependent manner PMID: 12538871
    21. data demonstrate that the effects of psychotomimetics on sensorimotor gating and repetitive movements depend on DARPP-32; they regulate, through distinct mechanisms, phosphorylation of DARPP-32 as well as downstream effectors in the cortex and striatum PMID: 14631045
    22. Blockade of the ERK pathway or mutation of DARPP-32 altered locomotor sensitization induced by a single injection of psychostimulants, demonstrating the functional relevance of this regulation. PMID: 15608059
    23. These data indicate a potential role for DARPP-32 and inhibitor-1 in the regulation of PP1 activity in pancreatic beta-cell stimulus-secretion coupling. PMID: 15737638
    24. these findings highlight distinct roles of the Thr-34 and Thr-75 phosphorylation sites of DARPP-32 in mediating short- and long-term behavioral and biochemical actions of cocaine PMID: 16123776
    25. Psychomotor effect of cannabinoids can be accounted for by striatal projection neurons involving protein kinase A-dependent phosphorylation of DARPP-32. PMID: 16162925
    26. DARPP-32 plays a key role for development of D1R motor stimulatory effects in mice PMID: 16189209
    27. The results indicate that DARPP-32 is involved in the retinal pathway transmitting photic information that resets the circadian clock. PMID: 16971527
    28. multiple signal transduction pathways mediating expression of DARPP-32 in vitro, including a novel, important pathway via by which PI3K regulates the contribution of cdk5/p35. PMID: 17209049
    29. These data indicate that DARPP-32 participates in dopamine-mediated modifications of retinal function. PMID: 17552992
    30. The abnormal involuntary movements developed in response to chronic L-DOPA are due to hyperactivation in striatal medium spiny neurons of a signaling pathway including sequential phosphorylation of DARPP-32, ERK1/2, MSK-1, and histone H3. PMID: 17596448
    31. although DARPP-32 impacts cortical inhibition through a previously demonstrated D2-dependent regulation of GABAergic currents in pyramidal cells, it is not involved in the direct D1-mediated regulation of fast-spiking interneurons PMID: 17693396
    32. DARPP-32 plays a central role in 1) modulating intercellular coupling, 2) cortical excitatory drive of spiny and aspiny GABAergic neurons, and 3) local feedforward inhibitory drive of cholinergic-like interneurons within accumbens circuits. PMID: 18155847
    33. The nuclear accumulation of DARPP-32, a potent inhibitor of protein phosphatase-1, increases the phosphorylation of histone H3, an important component of nucleosomal response. PMID: 18496528
    34. Csnk1-epsilon is crucial for the locomotor stimulant response to a moderate dose of MA and suggest that genetic polymorphisms affecting Csnk1-epsilon expression or function could influence sensitivity to amphetamines in both mice and humans. PMID: 19050854

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Protein phosphatase inhibitor 1 family
  • 数据库链接：

    KEGG: mmu:19049

    STRING: 10090.ENSMUSP00000077760

    UniGene: Mm.45372