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  4. Recombinant Mouse Protein flightless-1 homolog (Flii), partial

Recombinant Mouse Protein flightless-1 homolog (Flii), partial

  • 货号：
    CSB-EP884922MO
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    Flii
  • Uniprot No.：
  • 别名：
    Flii; Fli1; FliihProtein flightless-1 homolog
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    52.0kDa
  • 表达区域：
    495-827aa
  • 氨基酸序列
    VGQLPGLTIWQIENFVPVLVEEAFHGKFYEADCYIVLKTFLDDSGSLNWEIYYWIGGEATLDKKACSAIHAVNLRNYLGAECRTVREEMGDESEEFLQVFDNDISYIEGGTASGFYTVEDTHYVTRMYRVYGKKNIKLEPVPLKGSSLDPRFVFLLDQGLDIYVWRGAQATLSNTTKARLFAEKINKNERKGKAEITLLVQGQEPPGFWDVLGGEPSEIKNHVPDDFWPPQPKLYKVGLGLGYLELPQINYKLSVEHKKRPKVELMPGMRLLQSLLDTRCVYILDCWSDVFIWLGRKSPRLVRAAALKLGQELCGMLHRPRHTVVSRSLEGTE
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-B2M-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1. Involved in estrogen hormone signaling. Essential for early embryonic development. May play a role in regulation of cytoskeletal rearrangements involved in cytokinesis and cell migration, by inhibiting Rac1-dependent paxillin phosphorylation.
  • 基因功能参考文献：
    1. Fluorescence resonance energy transfer experiments showed that FliI-NMMIIA interactions require Ca(2+) influx. We conclude that Ca(2+) influx through the TRPV4 channel regulates FliI-NMMIIA interaction, which in turn enables generation of the cell extensions essential for collagen remodeling PMID: 28526784
    2. Flii genetic expression is enhances tissue regeneration, after claw amputation. PMID: 27595936
    3. Studies suggest that Flii enhances cutaneous squamous cell carcinoma progression by decreasing apoptosis and enhancing tumor cell invasion. PMID: 26497552
    4. P-Rex1 stimulates migration through enhancing the interaction between Rac1 and the actin-remodelling protein. PMID: 26887924
    5. Genes downstream from Flii, including TGF-beta1 and TGF-beta3, showed significantly altered expression confirming a functional effect of the Rhodamine-Flii small interfering RNA on gene expression PMID: 25838352
    6. FliI interacts with NMMIIA to promote cell extension formation, which enables collagen remodeling in fibroblasts. PMID: 25877872
    7. FLII functions in PPARgamma activation as a molecular switch to repress transcriptional activity by interrupting formation of the PPARgamma/RXRalpha complex. PMID: 25479590
    8. LRRFIP2 inhibits NLRP3 inflammasome activation by recruiting the caspase-1 inhibitor Flightless-I, thus outlining a new mechanism for negative regulation of NLRP3 inflammasome. PMID: 23942110
    9. increasing the level of Flii in diabetic mouse wounds led to increased TLR4 and NF- kappa B production. Treatment of murine diabetic wounds with neutralising antibodies to Flii led to an improvement in healing with decreased expression of TLR4 PMID: 23555084
    10. Using a mouse model of epidermolysis bullosa acquisita, the effect of "mopping up" Flii using Flii-neutralizing antibodies before, during, and after blister formation was determined. PMID: 23223144
    11. Flii is constitutively secreted from macrophages and fibroblasts and is present in human plasma. PMID: 22718342
    12. FliI regulates cell migration through its localization to focal adhesions and its ability to cap actin filaments, which collectively affect focal adhesion maturation. PMID: 22581781
    13. Data show that mice with elevated Flii expression exhibit impaired wound healing. PMID: 21786402
    14. Overexpression of Flii produced severe blistering post-induction of EBA, while decreased Flii reduced blister severity, elevated integrin expression, and improved ColVII production. PMID: 21984127
    15. show that Flii modulation of focal adhesions and filamentous actin stress fibers is Rac1-dependent PMID: 21430700
    16. Flii appears to have a positive role in the regeneration of hair follicles, contrary to its negative influence on wound healing in skin PMID: 21191408
    17. performs an essential function in early embryonic development PMID: 11971982
    18. Directly interferes with the formation of the TLR4-MyD88 signaling complex. PMID: 16424162
    19. FliI is a contributing factor to impaired healing and strategies aimed at decreasing FliI levels in elderly skin may improve wound repair. PMID: 18191609
    20. Flii may regulate wound repair through its effect on hemidesmosome formation and integrin-mediated cellular adhesion and migration. PMID: 19212345
    21. These findings support the model that CISK phosphorylates FLII and activates nuclear receptor transcription and suggest a new cell survival signaling pathway mediated by PI 3-kinase and CISK. PMID: 19293151

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  • 亚细胞定位：
    Nucleus. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cell junction, focal adhesion. Note=Colocalizes to actin-rich structures in blastocysts and, together with HRAS, RHOA and CDC42, in migrating fibroblasts. Localizes to centrosomes.
  • 组织特异性：
    Expressed in blastocyst.
  • 数据库链接：

    KEGG: mmu:14248

    STRING: 10090.ENSMUSP00000002889

    UniGene: Mm.339755