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  4. Recombinant Mouse Protein SET (Set)

Recombinant Mouse Protein SET (Set)

  • 货号：
    CSB-YP875345MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP875345MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP875345MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP875345MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP875345MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Set
  • Uniprot No.：
  • 别名：
    Set; Protein SET; Phosphatase 2A inhibitor I2PP2A; I-2PP2A; Template-activating factor I; TAF-I
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-289
  • 氨基酸序列
    APKRQSAIL PQPKKPRPAA APKLEDKSAS PGLPKGEKEQ QEAIEHIDEV QNEIDRLNEQ ASEEILKVEQ KYNKLRQPFF QKRSELIAKI PNFWVTTFVN HPQVSALLGE EDEEALHYLT RVEVTEFEDI KSGYRIDFYF DENPYFENKV LSKEFHLNES GDPSSKSTEI KWKSGKDLTK RSSQTQNKAS RKRQHEEPES FFTWFTDHSD AGADELGEVI KDDIWPNPLQ YYLVPDMDDE EGEAEDDDDD DEEEEGLEDI DEEGDEDEGE EDDDEDEGEE GEEDEGEDD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.
  • 基因功能参考文献：
    1. Molecular chaperone SET-assisted eviction of linker histones and Shugoshins is a fundamental step in mammalian mitotic progression. PMID: 28781233
    2. Presence of SET in the cytoplasm in Down Syndrome brains may play a role in the progression of the disease. PMID: 27460148
    3. Data suggest a critical role for the I2PP2A protein (SET)-protein phosphatase-2A (PP2A) signaling axis in Pten protein (Pten) deficient castration resistant prostate cancer (CRPC) progression. PMID: 26563471
    4. SET protein was expressed in spermatogonia and spermatocytes; the highest level was mainly in haploid and tetraploid cells of the prepubertal and adult groups, and Leydig cells of the adult and ageing groups. There was a low expression in Sertoli cells. PMID: 24923460
    5. The presence of full-length SET in the neuronal cytoplasm is sufficient to impair PP2A methylation and activity, leading to tau hyperphosphorylation. PMID: 24981783
    6. SET/PP2A system regulates testosterone production in ovarian follicles in vitro. PMID: 23628604
    7. Rsults suggest that SETbeta, even though it localizes to centromeres, might not be essential for chromosome separation during mouse oocyte meiotic maturation, although its forced overexpression causes premature chromatid separation. PMID: 23444375
    8. SET played a positive role in regulating ovarian androgen biosynthesis by enhancing the transcription of steroidogenic enzymes CYP17A1 and HSD3B2, which maybe contribute to the hyperandrogenism in polycystic ovary syndrome PMID: 23421880
    9. study found SET, a putative oncogene was significantly downregulated in a bisphenol A dose-dependent manner; from gene network analysis, SET is predicted to communicate with other genes including CYP17, which is involved in biosynthesis and metabolism of sex-hormones PMID: 22938446
    10. SET is an important regulator of chromosome condensation/decondensation and that disruption of the MCPH1-SET interaction might be important for the pathogenesis of primary microcephaly PMID: 21515671
    11. By a novel molecular mechanism, apolipoprotein (apo)E/peptide enhances protein phosphatase 2A (PP2A) activity within a cell by binding/forming a complex with SET, reducing the amount of SET available to inhibit PP2A, signaling, and inflammatory response. PMID: 21289314
    12. These studies indicated that the TAF-Ialpha promoter is under the control of Sp1.(TAF-Ialpha ) PMID: 18809386
    13. results from differential proteome analysis report a role for SET in Galpha(12)-mediated signaling pathways and a role for Galpha(12) in the regulation of the leukemia-associated SET-protein expression PMID: 15595726

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  • 亚细胞定位：
    Cytoplasm, cytosol. Endoplasmic reticulum. Nucleus, nucleoplasm.
  • 蛋白家族：
    Nucleosome assembly protein (NAP) family
  • 数据库链接：

    KEGG: mmu:56086

    STRING: 10090.ENSMUSP00000099930

    UniGene: Mm.326055