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  4. Recombinant Mouse Protection of telomeres protein 1 (Pot1)

Recombinant Mouse Protection of telomeres protein 1 (Pot1)

  • 货号：
    CSB-YP849657MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP849657MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP849657MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP849657MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP849657MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Pot1; Pot1a; Protection of telomeres protein 1; mPot1; POT1-like telomere end-binding protein
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-640
  • 氨基酸序列
    MSLVSTAPYT YTPLNLLKEG TIANVYGVVK FFKPPYVSKG TDYCSVVTIV DQTNVKLTCM LFSGNYEALP IIYKVGDIVR FHRLKIQVYK NELQGINCSG FASLTFEGTV GMPVTARTSS KVFSFTPQDQ KMVEALRVWA SKHISASSTL VQLCDAQPMQ YYDLTCQLLG KAQVDSTAFL LKVWDGTQTV LPSWRVSTQD LTFEGDLSHI ERLQSLVVDI LVYDNHVQVA RSIEVGCFLR LYSLHTKLQP GNSETSSSES LRLEFHLHGG TSYGRGIRVL PDTSPCVDQL KKALEGANLP VTETSTGICQ SENGDSSALS NSGSGAVSPY EEERCQQVSA TILTNHQHLE KTPLCAILTQ KAPQQYRVRA KLRSYLPRRL SQSVKLLCPK CHSVQEVPHG DSLDKILQDA ATEAPDIKLK ATSLYYSKVW TTEDQGGRQV AVHFVKNNGI LPASSECLIL IEGGRLCEVS KLSSKFHSVM PVRSGPESLE LLTLSAPFLI QGKVHHYGCK QCSSLKPIQN LNSRFHKGPW TPSSVAEALG VVPLQYVFVM VFTLDDGTGV LEAYLKDSEH FFKIPASEVL TDDDLQRSLE TIMDMICPPG IKVDAYPWLE CLLKSYNVTI GTERRICYQI FDTTVAENVV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA-binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'-TAGGGTTAG-3' sequence. Binds telomeric single-stranded sequences internally or at proximity of a 3'-end. Its activity is TERT dependent but it does not increase TERT activity.
  • 基因功能参考文献：
    1. The telomere binding protein POT1a inhibits the production of reactive oxygen species, and rejuvenates aged hematopoietic stem cells. PMID: 28986560
    2. proliferation of cancer cells lacking POT1 is enabled by the attenuation of the ATR kinase pathway. PMID: 27239034
    3. The results of this study found telomere uncapping by Pot1a inactivation resulted in an Atm-dependent loss of cerebellar interneurons and granule neuron precursors in the mouse nervous system. The PMID: 24899707
    4. shelterin protein TIN2 can protect chromosome ends as a TRF2-tethered TIN2/TPP1/POT1 complex that lacks a physical connection to TRF1 PMID: 24469404
    5. Simultaneous inactivation of Pot1a and p53 resulted in endometrial intraepithelial carcinoma-like lesions in transgenic mice. PMID: 22689059
    6. Telomere protection by TPP1/POT1 requires tethering to TIN2. PMID: 22099311
    7. Mouse gene deletion experiments revealed DNA-damage-response pathways that threaten chromosome ends and how the components of the telomeric shelterin complex prevent activation of these pathways.[Shelterin] PMID: 21209389
    8. The ability of mPot1 to unfold the antiparallel tetraplex of the mouse telomeric DNA is required for telomerase-mediated telomere elongation. PMID: 19749352
    9. Our results suggest that POT1a is crucial for the maintenance of both telomere integrity and overall genomic stability. PMID: 16839876
    10. We conclude that mouse telomeres require two distinct POT1 proteins whereas human telomeres have one. PMID: 16839877
    11. TRF2 and POT1 act independently to repress two DNA damage response pathways; TRF2 represses ATM, whereas POT1 prevents activation of ATR PMID: 17687332
    12. The ability of Pot1 to unfold the antiparallel tetraplex of the telomeric DNA may be important for regulation of telomerase-mediated telomere elongation. PMID: 18029680
    13. study reports that POT1a and POT1b do not differ in their ability to repress telomere recombination; the DNA binding domain of POT1a specifies its ability to repress the DNA damage response PMID: 18955498

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  • 亚细胞定位：
    Nucleus. Chromosome, telomere.
  • 蛋白家族：
    Telombin family
  • 数据库链接：

    KEGG: mmu:101185

    STRING: 10090.ENSMUSP00000110986

    UniGene: Mm.274589