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  4. Recombinant Mouse Prostaglandin G/H synthase 1 (Ptgs1)

Recombinant Mouse Prostaglandin G/H synthase 1 (Ptgs1)

  • 货号：
    CSB-YP018985MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP018985MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP018985MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP018985MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP018985MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Ptgs1
  • Uniprot No.：
  • 别名：
    Ptgs1; Cox-1; Cox1; Prostaglandin G/H synthase 1; EC 1.14.99.1; Cyclooxygenase-1; COX-1; Prostaglandin H2 synthase 1; PGH synthase 1; PGHS-1; PHS 1; Prostaglandin-endoperoxide synthase 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    27-602
  • 氨基酸序列
    ADPG VPSPVNPCCY YPCQNQGVCV RFGLDNYQCD CTRTGYSGPN CTIPEIWTWL RNSLRPSPSF THFLLTHGYW LWEFVNATFI REVLMRLVLT VRSNLIPSPP TYNSAHDYIS WESFSNVSYY TRILPSVPKD CPTPMGTKGK KQLPDVQLLA QQLLLRREFI PAPQGTNILF AFFAQHFTHQ FFKTSGKMGP GFTKALGHGV DLGHIYGDNL ERQYHLRLFK DGKLKYQVLD GEVYPPSVEQ ASVLMRYPPG VPPERQMAVG QEVFGLLPGL MLFSTIWLRE HNRVCDLLKE EHPTWDDEQL FQTTRLILIG ETIKIVIEEY VQHLSGYFLQ LKFDPELLFR AQFQYRNRIA MEFNHLYHWH PLMPNSFQVG SQEYSYEQFL FNTSMLVDYG VEALVDAFSR QRAGRIGGGR NFDYHVLHVA VDVIKESREM RLQPFNEYRK RFGLKPYTSF QELTGEKEMA AELEELYGDI DALEFYPGLL LEKCQPNSIF GESMIEMGAP FSLKGLLGNP ICSPEYWKPS TFGGDVGFNL VNTASLKKLV CLNTKTCPYV SFRVPDYPGD DGSVLVRRST EL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Dual cyclooxygenase and peroxidase in the biosynthesis pathway of prostanoids, a class of C20 oxylipins mainly derived from arachidonate, with a particular role in the inflammatory response. The cyclooxygenase activity oxygenates arachidonate (AA, C20:4(n-6)) to the hydroperoxy endoperoxide prostaglandin G2 (PGG2), and the peroxidase activity reduces PGG2 to the hydroxy endoperoxide PGH2, the precursor of all 2-series prostaglandins and thromboxanes. This complex transformation is initiated by abstraction of hydrogen at carbon 13 (with S-stereochemistry), followed by insertion of molecular O2 to form the endoperoxide bridge between carbon 9 and 11 that defines prostaglandins. The insertion of a second molecule of O2 (bis-oxygenase activity) yields a hydroperoxy group in PGG2 that is then reduced to PGH2 by two electrons. Involved in the constitutive production of prostanoids in particular in the stomach and platelets. In gastric epithelial cells, it is a key step in the generation of prostaglandins, such as prostaglandin E2 (PGE2), which plays an important role in cytoprotection. In platelets, it is involved in the generation of thromboxane A2 (TXA2), which promotes platelet activation and aggregation, vasoconstriction and proliferation of vascular smooth muscle cells.
  • 基因功能参考文献：
    1. IFNgamma upregulated microsomal prostaglandin E synthase-1 (mPGES-1) alongside cyclo-oxygenase (COX-1) within macrophage populations, resulting in sustained prostaglandin (PG)E2 biosynthesis. PMID: 28954232
    2. Data (including data from studies in knockout mice) suggest that delayed parturition in Cox-1 knockout mice is result of impaired luteolysis and cervical dilation, despite the presence of strong uterine contractions. PMID: 29029054
    3. Specific inhibition of PGE2 synthesis by targeting mPGES-1 may weaken host defense against bacterial infections. PMID: 27059285
    4. role of cyclooxygenase-1 and -2 in endothelium-dependent contraction of atherosclerotic mouse abdominal aortas PMID: 26444418
    5. Suggest the expression of COX-1 and COX-2 in the urothelium protects bladder damage from radiation. PMID: 27386631
    6. COX-1 inhibitor SC-560 has a protective effect on the thromboxane A2-mediated decrease in renal function in response to endotoxin. PMID: 26017977
    7. Expression of COX-1 is essential for the protection of liver against chemical-induced hepatotoxicity and required for hepatic homeostatic maintenance. PMID: 25432964
    8. Data suggest that multitarget FAAH/Cox blockade may provide a transformative approach to inflammatory bowel disease (IBD) and other pathologies in which fatty acid amide hydrolase/cyclooxygenases (FAAH, Cox-1, and Cox-2) are overactive. PMID: 25757568
    9. In arteries from non-insulin-dependent diabetic mice, COX-1 remains a major contributor to the endothelial PGI2 synthesis that evokes vasoconstrictor activity under the pathological condition. PMID: 24878773
    10. COX1/2-derived prostanoids appear to play an important role in the TLR response, with elevated cytokine levels following COX1/2 inhibition or genetic deletion. PMID: 23742950
    11. Data suggest deletion of Cox1 delays ovarian follicle development prior to reproductive maturation of mutant mouse strain (Wv); inhibition of Cox1 (but not Cox2) may prolong ovarian follicle lifespan and delay the onset of premature ovarian failure. PMID: 23966321
    12. role of COX-1 during maturation of adipocyte PMID: 23817787
    13. The production of prostanoids found in the systemic circulation is driven overwhelmingly by COX-1 and not COX-2. PMID: 23874970
    14. Prostaglandin G/H synthase is a moonlighting protein that functions both as a peroxidase as well as a cyclooxygenase. PMID: 8121489
    15. Hematopoietic COX-1 deletion results in impairments in metabolic homeostasis which may be partly due to increased adipose tissue inflammation and dysregulated adipokine profile. PMID: 23987235
    16. These observations suggest that COX-enzymes, most likely COX-1, are involved in cancer-elicited anorexia and weight loss PMID: 23305935
    17. KLF10 induces COX-1 protein expression and mRNA expression in endothelial cells. PMID: 23178857
    18. In the abdominal aorta, ACh evoked endothelium-dependent production of 6-keto-PGF(1alpha), which was abolished by COX-1(-/-), but not by COX-2(-/-). PMID: 22080487
    19. Niacin evoked platelet COX-1-derived PGD biosynthesis. PMID: 22406532
    20. Novel selective COX-1 inhibitors suppress neuroinflammatory mediators in LPS-stimulated N13 microglial cells. PMID: 22001217
    21. Astrocytes respond to lipopolysaccharide by a COX-2-dependent production of prostanoids, mainly vasoactive PGE(2), and suggest that the coordinated down-regulation of COX-1 facilitates PGE(2) production after TLR-4 activation. PMID: 22219191
    22. Data suggest that inhibition of COX-1, COX-2, or both enzymes alone seems inadequate to explain the gastric toxicity of piroxicam in mast cell-deficient mice. PMID: 21858200
    23. Cyclooxygenase-1 gene deltion resulted in an incresed proliferation and differentiation of hippocampal progenitor cells in the adult mouse brain druing neuroinflammation. PMID: 21694498
    24. COX-1 may play a crucial role in the pathology of neuroinflammation PMID: 21680698
    25. Cox-1-expressing cellular lineage is necessary to promote synovitis in vivo; Cox-1 activity is attributable to a bone marrow-derived lineage (hematopoietic) and not to a radioresistant mesenchymal (tissue) lineage. PMID: 21357261
    26. ErbB4-stimulated COX-2 induction is part of the molecular mechanisms responsible for ErbB4-induced cell survival PMID: 20585313
    27. A unique role for COX-1 in mediating chronic neuroinflammatory effects through PGE(2) production is suggested. PMID: 20412387
    28. These results indicate that inhibition of COX-1 activity may be valid therapeutic strategy to reduce brain inflammatory response and neurodegeneration. PMID: 20157512
    29. expressed in peritoneal macrophages and mast cells during acute peritonitis PMID: 19885646
    30. A novel mechanism was identified whereby chronic increases in oxidative stress, produced by mitochondrial superoxide dismutase deficiency, impair vascular function via a hydrogen peroxide-dependent, COX1-dependent, endothelium-derived contracting factor PMID: 20304815
    31. COX-1 and COX-2 seem to play an important role in renal ischemia and reperfusion injury, involving the secretion of pro-inflammatory cytokines, activation of neutrophils, and ROS production. PMID: 19711010
    32. our results demonstrate that endothelial cells control neuronal injury via mPGES-1-derived PGE2 PMID: 19658194
    33. These findings clearly demonstrate that the immediate corticosterone release seen after immune challenge with lipopolysaccharide is dependent on Cox-1 derived prostanoids PMID: 20034541
    34. The absence of COX-1 or COX-2 did not appear to effect ocular development in KO mice. PMID: 19767186
    35. In the hippocampus and cortex of LPS-treated mice, matrix metalloproteinase (MMP)-3 activity was significantly decreased in COX-1(-/-) mice PMID: 19844242
    36. These data indicate that genetic background significantly modifies the blood pressure response to mPGES1 deficiency. PMID: 20065147
    37. The tissue-specific, compensatory expression of cyclooxygenase-1 and -2 in transgenic mice. PMID: 11936618
    38. Opposite effects of cyclooxygenase-1 and -2 activity on the pressor response to angiotensin II. PMID: 12093889
    39. constitutive and inducible enzymes affect dermal wound healing PMID: 12118094
    40. after IL-1beta administration, COX1 is the major enzyme involved in the hypophagia PMID: 12183660
    41. Prostaglandins that increase renin production in response to ACE inhibition are not derived from cyclooxygenase-1. PMID: 12184998
    42. Results fail to confirm the harmful effect of losing COX-1 activity due to gene knockout in a permanent endovascular middle cerebral artery occlusion stroke model. PMID: 12213634
    43. Pre-epithelial alkaline layer is regulated by endogenous COX activity. PMID: 12411530
    44. Data show that both cyclooxygenase-1 and -2 isoforms are involved in the negative modulation of adipocyte differentiation. PMID: 12576525
    45. Site-specific expression in mouse kidney and relationship to prostaglandin metabolism. PMID: 12657565
    46. Knocking out this enzyme affects body temperature regulation. PMID: 12664644
    47. cyclooxygenase-1-coupled prostaglandin E2/D2 biosynthesis has a central role in skin repair PMID: 12713596
    48. mPGES-2 is a unique PGES that can be coupled with both COXs and may play a role in the production of the PGE2 involved in both tissue homeostasis and disease. PMID: 12835322
    49. PGE2 derived from either COX-1 or -2 is involved in regulation of gastric mucosal inflammation and contributes to maintenance of mucosal integrity during H. pylori infection via inhibition of TNF-alpha expression PMID: 12958020
    50. Like its counterpart COX-1, COX-3 does not generally appear to be induced by acute inflammatory stimulation of the CNS. PMID: 14625089

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  • 亚细胞定位：
    Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein.
  • 蛋白家族：
    Prostaglandin G/H synthase family
  • 数据库链接：

    KEGG: mmu:19224

    STRING: 10090.ENSMUSP00000059977

    UniGene: Mm.275434