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  4. Recombinant Mouse Proenkephalin-A (Penk)

Recombinant Mouse Proenkephalin-A (Penk)

  • 货号：
    CSB-YP017781MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP017781MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP017781MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP017781MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP017781MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Penk
  • Uniprot No.：
  • 别名：
    Penk; Penk1; Proenkephalin-A [Cleaved into: Synenkephalin; Met-enkephalin; Opioid growth factor; OGF); PENK(114-133); PENK(143-184); Met-enkephalin-Arg-Ser-Leu; Leu-enkephalin; PENK(238-259); Met-enkephalin-Arg-Phe]
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Cytoplasmic domain
  • 表达区域：
    25-97
  • 氨基酸序列
    ECSQDC AKCSYRLVRP GDINFLACTL ECEGQLPSFK IWETCKDLLQ VSRPEFPWDN IDMYKDSSKQ DESHLLA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Competes with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress.; Competes with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress.; Increases glutamate release in the striatum and decreases GABA concentration in the striatum.; Increases glutamate release in the striatum.
  • 基因功能参考文献：
    1. Nav1.7 deletion has profound effects on gene expression, leading to an upregulation of enkephalin precursor Penk mRNA and met-enkephalin protein in sensory neurons. PMID: 26634308
    2. PENK knockout exhibited fewer bouts of licking than wild types, even though the length of bouts was similar to that of wild types, a pattern that suggests diminished food motivation. PMID: 25754760
    3. PENK KO mice do not display anxiety and depression-related behavioral changes after chronic mild stress. PMID: 24804898
    4. upregulation of striatal enkephalin might have a role in early stage Huntington Disease in a mouse model PMID: 24040390
    5. Preproenkephalin-enhanced green fluorescent protein (GFP) transgenic mice were used to study ENKergic neuron distribution patterns. PMID: 22067363
    6. ENKephalinergic neurons develop according to a rostrocaudal and ventrodorsal gradient. PMID: 21124002
    7. Met-enkephalin levels in neuronal projections from entorhinal cortex and dentate gyrus were increased, as were preproenkephalin mRNA levels. Elevations may contribute to cognitive impairments. (Met-enkephalin, preproenkephalin) PMID: 18463254
    8. c-fos expression in specific brain areas in preproenkephalin deficiency PMID: 20406487
    9. Preproenkephalin deletion in Hyp mice (Phex-deficient) partially rescues their bone mineralization defect.A role for Penk in osteoblast differentiation. PMID: 20204609
    10. Morphine dependence is examined in preproenkephalin knockout mice. PMID: 12486185
    11. A defect in activation seen in preproenkephalin (Penk)-deficient T cells results in a reduced number of cells entering into cell cycle; proliferation can be rescued by increasing antigen or by co-incubation of Penk-deficient cells with wild-type cells. PMID: 12864972
    12. mice with the Penk1 mutation in the DBA/2J, but not on other genetic backgrounds, showed an increased opioid-dependent stress-induced analgesia PMID: 15197532
    13. Endogenous opioid peptides derived from preproenkephalin are involved in the antinociceptive and rewarding properties of nicotine and participate in the expression of physical nicotine dependence. PMID: 15689546
    14. These data implicate enkephalin as a signaling molecule in the control of hypoxic depression of metabolism in mice. PMID: 16095981
    15. Preproenkephalin knockout mice show no depression-related phenotype. PMID: 17375141
    16. Increase in ACTH level after zero-maze test failed to reach the level of significance in Penk1(-/-) mice. PMID: 18280051
    17. Preproenkephalin and GABA colocalized in a subpopulation of neurons in the spinal dorsal horn. They are likely to represent local inhibitory dorsal horn interneurons involved in the modulation of pain transmission. PMID: 18780300
    18. As PPE-derived peptides are endogenous ligands for MOR in the neostriatum and few axon collaterals of matrix neurons enter the striosome compartment, the present results raised the question of the target of those peptides produced by matrix neurons. PMID: 19046386
    19. Administration of the antagonist prevented the acquisition of MDMA-induced CPP, suggesting an implication of the delta opioid receptors in this behavior. PMID: 19523041

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  • 亚细胞定位：
    Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen. Secreted.
  • 蛋白家族：
    Opioid neuropeptide precursor family
  • 组织特异性：
    Spermatogenic and somatic cells.
  • 数据库链接：

    KEGG: mmu:18619

    STRING: 10090.ENSMUSP00000066822

    UniGene: Mm.475097