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  4. Recombinant Mouse Polymerase I and transcript release factor (Ptrf)

Recombinant Mouse Polymerase I and transcript release factor (Ptrf)

  • 货号：
    CSB-YP019070MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP019070MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP019070MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP019070MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP019070MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Cavin1
  • Uniprot No.：
  • 别名：
    Cavin1; Ptrf; Caveolae-associated protein 1; Cav-p60; Cavin-1; Polymerase I and transcript release factor
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-392
  • 氨基酸序列
    MEDVTLHIVE RPYSGFPDAS SEGPEPTQGE ARATEEPSGT GSDELIKSDQ VNGVLVLSLL DKIIGAVDQI QLTQAQLEER QAEMEGAVQS IQGELSKLGK AHATTSNTVS KLLEKVRKVS VNVKTVRGSL ERQAGQIKKL EVNEAELLRR RNFKVMIYQD EVKLPAKLSV SKSLKESEAL PEKEGDELGE GERPEDDTAA IELSSDEAVE VEEVIEESRA ERIKRSGLRR VDDFKKAFSK EKMEKTKVRT RENLEKTRLK TKENLEKTRH TLEKRMNKLG TRLVPVERRE KLKTSRDKLR KSFTPDHVVY ARSKTAVYKV PPFTFHVKKI REGEVEVLKA TEMVEVGPED DEVGAERGEA TDLLRGSSPD VHTLLEITEE SDAVLVDKSD SD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays an important role in caveolae formation and organization. Essential for the formation of caveolae in all tissues. Core component of the CAVIN complex which is essential for recruitment of the complex to the caveolae in presence of calveolin-1 (CAV1). Essential for normal oligomerization of CAV1. Promotes ribosomal transcriptional activity in response to metabolic challenges in the adipocytes and plays an important role in the formation of the ribosomal transcriptional loop. Dissociates transcription complexes paused by DNA-bound TTF1, thereby releasing both RNA polymerase I and pre-RNA from the template. The caveolae biogenesis pathway is required for the secretion of proteins such as GASK1A.
  • 基因功能参考文献：
    1. The s show here in mature adipocytes, ribosomal transcription can be acutely regulated in response to metabolic challenges. This acute response is mediated by PTRF (polymerase I transcription and release factor, also known as cavin-1), which has previously been shown to play a critical role in caveolae formation. PMID: 27528195
    2. The current analyses reveal roles for cavin-1 and related proteins in governing myocardial contractile function and compliance, coronary and cardiac responses to mechanical and ischaemic stressors, and membrane permeability/fragility PMID: 28343262
    3. acetylated cavin-1 preferentially interacted with hormone-sensitive lipase and recruited it to the caveolae, thereby promoting lipolysis. PMID: 28559430
    4. these results suggested that the membrane dynamics in cell migration is affected by caveolae associated PTRF/cavin-1. PMID: 25514038
    5. study demonstrates a critical role of cavin-1 in vascular structure and function, but the influences on arterial resistance cancel each other such that arterial blood pressure remains unaltered in homeostatic conditions PMID: 24658465
    6. lipid mobilization in cultured adipocytes to induce lipid droplet shrinkage led to biphasic response of cavin-1 with ultimate loss of expression of cavin-1 by protein degradation. PMID: 24969108
    7. A Western-type diet in apo-E2 knock-in mice produced 3 groups of obese mice with PTRF expression correlating with glucose tolerance: normal, hyperinsulinemic glucose-tolerant, or impaired. PTRF compromised adipocyte differentiation of 3T3-L1 cells. PMID: 24812087
    8. Adipocytes from cavin-1-null mice are markedly dysfunctional exhibiting decreased insulin-dependent glucose uptake, fatty acid uptake, and lipolysis. PMID: 24509860
    9. PTRF is a crucial regulator of TLR4 signaling in the development of sepsis. PMID: 23908488
    10. an important role for Cavin1 in lung homeostasis PMID: 23634221
    11. Cavin-1 is critical for detrusor caveolae and for overall contractility and structure of the urinary bladder. PMID: 22643325
    12. Cavin-1 modulates cellular polarization, and the subcellular localization of Rac1 and caveolin-1 in migrating cells as well as PKCalpha caveola recruitment. PMID: 22912783
    13. Double-immunogold labeling showed that the caveolae-shaping molecule PTRF/cavin 1 behaved similarly and that syndapin II and PTRF/cavin 1 colocalized. PMID: 22718246
    14. membrane-delimited interaction between MG53 and PTRF contributes to initiation of cell membrane repair PMID: 21343302
    15. Our study is the first direct demonstration for a novel adipose tissue-specific function of PTRF as a mediator of lipolysis and also shows that phosphorylation of PTRF is required for efficient fat mobilization. PMID: 21282370
    16. We conclude that PTRF-Cavin is required for caveola formation and sequestration of mobile caveolin into immobile caveolae. PMID: 18191225

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  • 亚细胞定位：
    Membrane, caveola. Cell membrane. Microsome. Endoplasmic reticulum. Cytoplasm, cytosol. Mitochondrion. Nucleus.
  • 蛋白家族：
    CAVIN family
  • 组织特异性：
    Expressed in the heart, stomach, adipose tissue and lung (at protein level). Expressed in testis, kidney, muscle, liver, spleen and brain.
  • 数据库链接：

    KEGG: mmu:19285

    STRING: 10090.ENSMUSP00000058321

    UniGene: Mm.21864