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  4. Recombinant Mouse Polycomb group RING finger protein 6 (Pcgf6)

Recombinant Mouse Polycomb group RING finger protein 6 (Pcgf6)

  • 中文名称：
    小鼠Pcgf6重组蛋白
  • 货号：
    CSB-YP860407MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Pcgf6重组蛋白
  • 货号：
    CSB-EP860407MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Pcgf6重组蛋白
  • 货号：
    CSB-EP860407MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Pcgf6重组蛋白
  • 货号：
    CSB-BP860407MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Pcgf6重组蛋白
  • 货号：
    CSB-MP860407MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Pcgf6; Mblr; Rnf134; Polycomb group RING finger protein 6; Mel18 and Bmi1-like RING finger; RING finger protein 134
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-353
  • 氨基酸序列
    MDEAETDATE NKRASEAKRA SAMPPPPPPP PPISPPALIP APAAGEEGPA SLGQAGAAGC SRSRPPALEP ERSLGRLRGR FEDYDEELEE EEEMEEEEEE EEEMSHFSLR LESGRADSED EEERLINLVE LTPYILCSIC KGYLIDATTI TECLHTFCKS CIVRHFYYSN RCPKCNIVVH QTQPLYNIRL DRQLQDIVYK LVINLEEREK KQMHDFYKER GLEVPKPAAP QPVPSSKGKT KKVLESVFRI PPELDMSLLL EFIGANEDTG HFKPLEKKFV RVSGEATIGH VEKFLRRKMG LDPACQVDII CGDHLLERYQ TLREIRRAIG DTAMQDGLLV LHYGLVVSPL KIT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional repressor. May modulate the levels of histone H3K4Me3 by activating KDM5D histone demethylase. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity.
  • 基因功能参考文献：
    1. Pcgf6 acts as a master regulator to maintain embryonic stem cell identity. PMID: 27247273
    2. Conditional ablation of Pcgf6 in embryonic stem cells leads to robust de-repression of germ cell-related genes, in turn affecting cell growth and viability. PMID: 28304275
    3. The results identify two key regulators, PCGF6 and JARID1c that temper dendritic cells (DC) activation and implicate active transcriptional silencing via histone demethylation as a previously unappreciated mechanism for regulating DC activation and quiescence. PMID: 27498878
    4. Pcgf6 is essential for recruitment of PRC1.6 to chromatin. Our results reveal a previously uncharacterized, H2AK119ub1-independent chromatin assembly associated with PRC1.6 complex PMID: 28049731
    5. that Pcgf6 is nonredundantly involved in maintaining the pluripotent nature of embryonic stem cells and it functions in induced pluripotent stem reprogramming PMID: 25187489
    6. regulation by cell cycle-dependent phosphorylation PMID: 12167161

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Expressed in ovary, testis, stomach, liver, thymus and kidney (at protein level).
  • 数据库链接：

    KEGG: mmu:71041

    STRING: 10090.ENSMUSP00000026032

    UniGene: Mm.35413