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  4. Recombinant Mouse Polycomb complex protein BMI-1 (Bmi1)

Recombinant Mouse Polycomb complex protein BMI-1 (Bmi1)

  • 货号：
    CSB-YP002726MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP002726MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP002726MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP002726MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP002726MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Bmi1; Bmi-1; Pcgf4Polycomb complex protein BMI-1; Polycomb group RING finger protein 4
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-324
  • 氨基酸序列
    MHRTTRIKIT ELNPHLMCVL CGGYFIDATT IIECLHSFCK TCIVRYLETS KYCPICDVQV HKTRPLLNIR SDKTLQDIVY KLVPGLFKNE MKRRRDFYAA HPSADAANGS NEDRGEVADE EKRIITDDEI ISLSIEFFDQ SRLDRKVNKE KPKEEVNDKR YLRCPAAMTV MHLRKFLRSK MDIPNTFQID VMYEEEPLKD YYTLMDIAYI YTWRRNGPLP LKYRVRPTCK RMKMSHQRDG LTNAGELESD SGSDKANSPA GGVPSTSSCL PSPSTPVQSP HPQFPHISST MNGTSNSPSA NHQSSFASRP RKSSLNGSSA TSSG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A. In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2.
  • 基因功能参考文献：
    1. Bmi1 is a marker for a distinct population of castration-resistant luminal epithelial cells enriched in the proximal prostate that can serve as a cell of origin for prostate cancer PMID: 27703144
    2. Findings extend current knowledge of the role of BMI1 and CHD7 in medulloblastoma pathogenesis, and they raise the possibility that pharmacological targeting of BMI1 or ERK may be particularly indicated in a subgroup of MB with low expression levels of CHD7 PMID: 29212025
    3. Many Bmi1-positive cells within the tongue cancer specimens failed to proliferate. PMID: 28004815
    4. High Expressions of BMI1 is associated with breast cancer. PMID: 28418883
    5. of Bmi1 in lymphocytes can stimulate osteogenesis in vivo and partially rescue defects in skeletal growth and osteogenesis. PMID: 27373231
    6. miR-203 is repressed by EZH2 in both embryonic and adult neural stem/progenitor cells (NSPCs). MiR-203 negatively regulates the proliferation of NSPCs. One of PRC1 components, Bmi1, is a downstream target of miR-203 in NSPCs. PMID: 28602614
    7. Data suggest BMI1 overexpression as a novel mechanism leading to EphA7 inactivation via H3K27 trimethylation and DNA methylation by which BMI-1 controls cell proliferation in the postnatal lateral ventricle wall. PMID: 27533460
    8. Bmi1 plays an important role in regulating the proliferation of cochlear supporting cells. PMID: 26843109
    9. Results from this study indicate that estrogen deficiency downregulates BMI-1 and subsequently increases ROS, T cell activation, and RANKL production in T cells, thus enhancing osteoclastogenesis and accelerating bone loss. PMID: 27943387
    10. ompounding a previously described Bmi1-transgene and Pten-deficiency prostate cancer mouse model with the Ezh2 transgene did not enhance tumour progression or drive metastasis formation. In conclusion, we here report the generation of a wildtype Ezh2 overexpression mouse model that allows for intravital surveillance of tissues with activated transgene PMID: 27807665
    11. BMI1 and MEL18 contribute to the development of colitis-associated cancer in mice by promoting proliferation and reducing apoptosis via suppressing expression of Reg3b. REG3B negatively regulates cytokine-induced activation of STAT3 in colon epithelial cells. PMID: 28780076
    12. Data show that C/EBPalpha is a tumor suppressor in lung cancer and that BMI1 is required for the oncogenic process downstream of C/EBPalpha loss. PMID: 27488898
    13. KLF4 modulates development of BMI1-expressing intestinal stem cell-derived lineage following gamma-radiation-induced gut injury in mice. PMID: 27237377
    14. The retinal phenotype of Bmi1(-/-) mice was characterized by loss of heterochromatin, activation of tandem repeats, oxidative stress and Rip3-associated necroptosis. PMID: 26965367
    15. Loss of BMI1 enhanced ribonuclease activity of polynucleotide phosphorylase and reduced mtRNA stability PMID: 27613804
    16. Inducible fate mapping demonstrates that BMI1 is expressed in vivo by multipotent Olfactory epithelium progenitors. expression of BMI1 and other Polycomb proteins not previously identified in olfactory basal cells are essential for self-renewal. PMID: 27789621
    17. We conclude that silencing of Bmi1 by miR-27b relieves repression of the presynaptic transcriptome and supports neurotransmission in cortical networks. PMID: 27716060
    18. a full complement of Bmi-1 is required for the intestinal proliferative effects of GLP-2 in both the physiological and pathological setting PMID: 27187177
    19. Our work revealed that MOZ and BMI1 regulate HSCs in a synergistic manner by acting on distinct processes required to maintain HSCs. PMID: 27773671
    20. Bmi-1 played a critical role in the protection from acute tubular necrosis by maintaining mobilization of renal stem cells and would be a novel therapeutic target for preventing the progression of ATN PMID: 27871857
    21. Bmi1 deletion could substitute for Gata4 during iCM reprogramming. Thus, Bmi1 acts as a critical epigenetic barrier to iCM production. Bypassing this barrier simplifies iCM generation and increases yield, potentially streamlining iCM production for therapeutic purposes. PMID: 26942853
    22. Cardiac Bmi1 progenitor cells respond to cardiac injury, contributing to the generation of de novo cardiomyocytes in the adult mouse heart. PMID: 27472922
    23. Bmi1 is a potential driver oncogene of bladder cancer, and it may become a potential treatment target for human bladder cancer. PMID: 26489630
    24. High Bmi1 expression identifies a non-cardiomyocyte resident cardiac population that contributes to the main lineages of the heart in vitro and in vivo. PMID: 26503423
    25. Here we show that inactivation of BMI1 using specific siRNA slows and decreases the levels of adipocyte differentiation , but does not abolish it. PMID: 27228653
    26. These observations suggest a dynamic and developmentally regulated model of PRC1 occupancy at constitutive heterochromatin, and where BMI1 function in somatic cells is to stabilize the repetitive genome PMID: 26468281
    27. Hypoxia models showed the mechanism by which hypoxia promoted Twist1 and Bmi1 expression and led to vasculogenic mimicry formation. PMID: 26202447
    28. Dysregulation of the Bmi-1/p16Ink4a pathway provokes an aging-associated decline of submandibular gland function. PMID: 25832744
    29. Bmi-1 overexpression conferred extensive self-renewal capacity upon adult bone-marrow-derived self-renewing erythroblasts, which normally have limited proliferative potential. PMID: 26028528
    30. Lgr6 and Bmi1 have roles in stem cells in the acral epithelium that participate in the long-term maintenance of sweat glands, ducts, and interadnexal epidermis, while Lgr5 has a role in rapid cycling and maintance of sweat glands only PMID: 26362184
    31. BMI1 expression in human hematopoietic stem and progenitor cells from patients with DBA is correlated with the expression of some ribosomal protein genes, suggesting that BMI1 deficiency may play a pathological role in DBA and other ribosomopathies. PMID: 25385494
    32. Sin3B is required for the senescent phenotype and elevated levels of reactive oxygen species elicited upon Bmi-1 depletion. PMID: 25263442
    33. BMI1 is required for the initiation of murine pancreatic neoplasia through an Ink4a-independent mechanism. PMID: 25939753
    34. Quercetin attenuates doxorubicin cardiotoxicity by modulating Bmi-1 expression PMID: 24902966
    35. The molecular mechanism underlying enhanced physiological function of Bmi1 depends on the injury context and it is mediated by metallothionein 1 (MT1)-driven modulation of resistance to oxidative stress in the satellite cell population. PMID: 25452464
    36. Two different BMI1-containing PcG complexes regulate hematopoietic stem cell activity, which are distinguishable by the presence of UBAP2L. PMID: 25185265
    37. Bmi1, which is expressed in the intestinal stem cells and progenitor compartments, was identified as a gene that is co-regulated by Notch and beta-catenin. PMID: 25480918
    38. Data indicate that forced over-expression of miR-200c decreased the level of BMI1 protein, indicating BMI1 is a direct mediator of miR-200c functions. PMID: 24375660
    39. Study concludes that only the 53BP1 status in DNA lesions, induced by UVA or gamma-rays, is affected by A-type lamin deficiency, which was not observed for heterochromatin-related proteins HP1beta and BMI1. PMID: 24611931
    40. Antagonistic interplay between necdin and Bmi1 controls proliferation of neural precursor cells in the embryonic mouse neocortex. PMID: 24392139
    41. stem renewal factor Bmi-1 was a direct target of miR-203 PMID: 24219349
    42. Bmi-1 might modulate the growth of lung adenocarcinoma cells in an INK4a-p16 independent pathway. PMID: 24552182
    43. Heterozygous Bmi-1 gene knockout causes an early onset of age-related brain changes, suggesting that Bmi-1 has a role in regulating brain aging. PMID: 23771506
    44. Bmi1 deficiency impairs the progression and maintenance of small intestinal tumors in a cell autonomous and highly Arf-dependent manner PMID: 23955081
    45. Because miR-200 represses Bmi1, induction of ZEB1 leads to induction of Bmi1. Rb1 pathway status then dictates the opposing effects of mutant Ras on the ZEB1-miR-200 loop in primary versus cancer cells. PMID: 24371144
    46. a general mechanism for p53 inactivation in some embryonal cell types and consequent susceptibility to MycN oncogenesis at the point of embryonal tumor initiation PMID: 22907436
    47. Bmi1 is important in the cellular response to the transforming growth factor-beta/bone morphogenetic protein (TGF-beta/BMP) and endoplasmic reticulum (ER) stress pathways. PMID: 23680149
    48. overexpression of Bmi1 induces repressive epigenetic regulation of the promoter of Survivin, a well-characterized antiapoptotic protein. PMID: 23132836
    49. Overexpression of Bmi1 in granule cell progenitors (GCPs) led to a decrease in cerebellar size due to decreased GCP proliferation and repression of cyclin genes, whereas Bmi1 overexpression in postmitotic granule cells improved cell survival. PMID: 23065639
    50. Retinal degeneration depends on Bmi1 function and reactivation of cell cycle proteins. PMID: 23359713

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 组织特异性：
    Detected in most organs with high expression levels in thymus, heart, brain and testis.
  • 数据库链接：

    KEGG: mmu:12151

    STRING: 10090.ENSMUSP00000028071

    UniGene: Mm.289584