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  4. Recombinant Mouse Platelet-derived growth factor subunit B (Pdgfb)

Recombinant Mouse Platelet-derived growth factor subunit B (Pdgfb)

  • 货号：
    CSB-YP017709MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP017709MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP017709MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP017709MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Pdgfb; SisPlatelet-derived growth factor subunit B; PDGF subunit B; PDGF-2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; Proto-oncogene c-Sis
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    82-190
  • 氨基酸序列
    SLGSLAAAE PAVIAECKTR TEVFQISRNL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETIVTPRPVT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA.
  • 基因功能参考文献：
    1. microRNA let-7g suppresses PDGF-induced conversion of vascular smooth muscle cell into the atherosclerotic phenotype. PMID: 28699690
    2. The PDGFB-overexpressing murine tumors closely cluster with human proneural and mesenchymal subtypes, as well as PDGFRA-amplified at both the RNA and protein expression levels. These models can be generated in fully immunocompetent mixed or C57BL/6 genetic background mice, and therefore can easily be incorporated into preclinical studies for cancer cell-specific or immune cell-targeting drug discovery studies. PMID: 28836293
    3. Myeloid cell-derived PDGF contributes to vascular neotissue formation by regulating macrophage apoptosis, smooth muscle cell proliferation and extracellular matrix deposition. PMID: 28524773
    4. These results demonstrate the requirement of lymphatic endothelial cells-autonomous PDGFB expression and retention for smooth muscle cell recruitment to lymphatic vessels. PMID: 28851707
    5. Mechanical strain stimulates vasculogenesis of embryonic stem cells by the intracellular messengers ROS, NO and calcium as well as by upregulation of angiogenesis guidance molecules and the angiogenic growth factors VEGF, FGF-2 and PDGF-BB. PMID: 27725190
    6. PDGF-B-PDGFRbeta signaling plays a significant role in the development of adipose tissue neovascularization. PMID: 28122789
    7. data suggest that the stronger fibrotic effect generated by Pdgfa overexpression was mediated by Pdgfralpha in cardiac interstitial mesenchymal cells PMID: 27816607
    8. loss-of-function mutations in PDGFB or PDGFRB cause Primary Familial Brain Calcification. PMID: 26599395
    9. High PDGFB expression is associated with liver fibrosis. PMID: 24801603
    10. Increased expression of STAT5b + PDGFB led to increased expression of downstream STAT5b targets. PMID: 25302990
    11. Platelets produce PDGFB to activate stellate cells and promote fibrosis in MDR2-null mice. PMID: 25173753
    12. The endogenous levels of PDGF-BB, increases CD31(hi)endomucin(hi) vessel number and stimulates bone formation in ovariectomy mice. PMID: 25282358
    13. These findings thus implicate a novel role of PDGF-BB in the migration of pericytes, resulting in loss of pericyte coverage from the endothelium with a subsequent breach of the blood-brain barrier PMID: 25164676
    14. The signaling pathways of VEGF and PDGF are crucial mediators for determining proliferation of endothelial cells and oligodendrocytes in the neurohypophysis of adult mice. PMID: 24860149
    15. Our findings demonstrate, for the first time, that Shh is involved in PDGF-BB-induced smooth muscle cell migration and recruitment of mural cells into neovessels PMID: 24472833
    16. Tumor PDGF-BB expression levels determine dual effects of anti-PDGF drugs on vascular remodelling and metastasis. PMID: 23831851
    17. Mutations in the gene encoding PDGF-B cause brain calcifications in humans and mice. PMID: 23913003
    18. endothelium, via Dll4 and PDGF-BB, induces a fate switch in adjacent skeletal myoblasts PMID: 23477786
    19. Development of the retina is severely disturbed in transgenic mice in which PDGF-B was over-expressed . PMID: 22880002
    20. PDGF-B signaling in trophoblasts was identified as a key component of the unique placental hematopoietic microenvironment that protects hematopoietic stem cells from premature differentiation. PMID: 22387002
    21. define a previously unidentified mechanism of PDGF-BB-induced tumor angiogenesis and provide compelling evidence for the key role of EPO in mediating PDGF-BB-induced angiogenesis, tumor growth and hematopoiesis PMID: 22138754
    22. TGFbeta and PDGF-B have important roles in modulating myofibroblast generation in the mouse cornea after haze-associated injury. PMID: 21978952
    23. PDGF-BB induces ASM hyperplasia and changes in lung mechanics in mice PMID: 21216974
    24. this is the first study to connect PDGF-B-induced changes in hepatic sinusoidal transport to changes in insulin action, demonstrating a link between PDGF-B signaling and insulin sensitivity. PMID: 21673305
    25. Data show that PDGF-B depletion by RNAi caused GICs to lose their tumor-initiating capacity. PMID: 21677873
    26. PDGF-BB stimulates cultured cardiomyocytes to synthesize the extracellular matrix component hyaluronan via HAS2 PMID: 21200430
    27. Overexpression of platelet-derived growth factor-B accelerates liver cancer development in chemically induced liver carcinogenesis. PMID: 20506153
    28. PDGF-B-driven gliomagenesis can occur in the absence of the proteoglycan NG2 PMID: 20939912
    29. a tightly regulated expression of PDGF during embryogenesis is required for normal brain development and function in mice. PMID: 20643125
    30. PDGF has a role in induction of the synaptic plasticity gene Arc/Arg3.1 PMID: 20452974
    31. The COL1A1-PDGFB fusion gene is the most characteristic cytogenic anomaly in DFSP. PMID: 20133221
    32. study to examine the potential role of PDGF-B in an experimental model of peritoneal fibrosis PMID: 19896531
    33. results show that osteoclasts regulate osteoblasts directly and suggest that PDGF BB is a key factor in bone remodeling PMID: 11811556
    34. PDGF-B chain transformation of NIH3T3 cells requires activation of NF-kappaB PMID: 11855867
    35. endothelium-restricted ablation of PDGF-B generates viable mice with variation in the density of pericytes throughout the CNS; data suggest that a reduction of the pericyte density is sufficient to cause retinopathy in mice PMID: 12169633
    36. Blockade transiently delays but does not prevent fibrous cap formation in ApoE null mice PMID: 12368212
    37. PDGF is an important effector molecule in postnatal retinal development. PMID: 12528182
    38. retention of PDGF-B in microvessels is essential for proper recruitment and organization of pericytes and for renal and retinal function in adult mice PMID: 12897053
    39. Endothelial and nonendothelial sources of PDGF-B regulate pericyte recruitment and influence vascular pattern formation in tumors. PMID: 14561699
    40. PDGF-BB stimulation of fibroblast-collagen interactions is mediated by the alphavbeta3 integrin when beta1 integrin function is impaired. PMID: 14644167
    41. In vitro cultures supporting the spontaneous generation of functional cardiac myocytes from murine BMCs demonstrated induced expression of PDGF)-A and -B. PDGF-AB enhances the generation of BMC-derived cardiac myocytes in mouse hearts. PMID: 14963008
    42. Imatinib reduces PDGFB by treating the atherosclerosis or by an autocrine loop linking PDGF signal transduction to PDGF expression. PDGF plays a central role in the complex interplay of cytokines & growth factors leading to a vicious circle. PMID: 14988091
    43. expression and potential role of platelet-derived growth factor-BB (PDGF-BB) and its receptor, PDGF receptor beta (PDGFR-beta), during testis cord formation PMID: 14996938
    44. PDGF-B provides a key switch from cell death to survival induced by TNF PMID: 15735680
    45. syngenic mice deficient of PDGF-B leading to a defect in the recruitment of perivascular cells PMID: 15838875
    46. PDGF-BB induces phosphorylation of alphaB-crystallin via p38 MAP kinase during myocardial infarction PMID: 15907784
    47. Our studies identify two independent negative immune regulatory pathways-PDGF-B and SOCS-that may help limit lesion expansion. PMID: 16127167
    48. PDGF-BB and TGF-beta1 binding to intact alpha2M is specific, involving a defined region of the alpha2M subunit PMID: 16641085
    49. platelet-derived growth factor B has a role in anti-vascular endothelial growth factor therapy in multiple models of ocular neovascularization PMID: 16723717
    50. PDGF-B overexpression causes liver fibrosis without significantly upregulating TGF-beta1, suggesting a TGF-beta-independent mechanism. PMID: 16842882

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    PDGF/VEGF growth factor family
  • 组织特异性：
    Localized to vascular smooth muscle cells. Also weakly expressed by cortical interstitial cells but absent in tubules. Up-regulated in areas of renal fibrosis. In mice with unilateral ureteral obstruction, an increased expression in interstitial cells and
  • 数据库链接：

    KEGG: mmu:18591

    STRING: 10090.ENSMUSP00000000500

    UniGene: Mm.144089