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  4. Recombinant Mouse Pancreas transcription factor 1 subunit alpha (Ptf1a)

Recombinant Mouse Pancreas transcription factor 1 subunit alpha (Ptf1a)

  • 中文名称：
    小鼠Ptf1a重组蛋白
  • 货号：
    CSB-YP879436MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Ptf1a重组蛋白
  • 货号：
    CSB-EP879436MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Ptf1a重组蛋白
  • 货号：
    CSB-EP879436MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Ptf1a重组蛋白
  • 货号：
    CSB-BP879436MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Ptf1a重组蛋白
  • 货号：
    CSB-MP879436MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Ptf1a
  • Uniprot No.：
  • 别名：
    Ptf1a; Ptf1p48; Pancreas transcription factor 1 subunit alpha; Pancreas-specific transcription factor 1a; bHLH transcription factor p48; p48 DNA-binding subunit of transcription factor PTF1; PTF1-p48
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-324
  • 氨基酸序列
    MDAVLLEHFP GGLDTFPSPY FDEEDFFTDQ SSRDPLEDSD ELLGDEQAEV EFLSHQLHEY CYRDGACLLL QPAPSAAPHA LAPPPLGDPG EPEDNVSYCC DAGAPLAAFP YSPGSPPSCL AYPCAAVLSP GARLGGLNGA AAAAAARRRR RVRSEAELQQ LRQAANVRER RRMQSINDAF EGLRSHIPTL PYEKRLSKVD TLRLAIGYIN FLSELVQADL PLRGSGAGGC GGPGGSRHLG EDSPGNQAQK VIICHRGTRS PSPSDPDYGL PPLAGHSLSW TDEKQLKEQN IIRTAKVWTP EDPRKLNSKS FDNIENEPPF EFVS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor implicated in the cell fate determination in various organs. Binds to the E-box consensus sequence 5'-CANNTG-3'. Plays a role in early and late pancreas development and differentiation. Important for determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. May be involved in the maintenance of exocrine pancreas-specific gene expression including ELA1 and amylase. Required for the formation of pancreatic acinar and ductal cells. Plays an important role in cerebellar development. Directly regulated by FOXN4 and RORC during retinal development, FOXN4-PTF1A pathway plays a central role in directing the differentiation of retinal progenitors towards horizontal and amacrine fates.
  • 基因功能参考文献：
    1. non-neural progenitor transcription factor Ptf1a alone is sufficient to directly reprogram mouse and human fibroblasts into self-renewable induced neural stem cells capable of differentiating into functional neurons, astrocytes and oligodendrocytes, and improving cognitive dysfunction of Alzheimer's disease mouse models when transplanted PMID: 30030434
    2. Ptf1a is regulated by active and repressive inputs integrated through multiple sequence elements within a highly conserved sequence downstream of the Ptf1a gene. PMID: 27350561
    3. the Tfap2a and Tfap2b transcription factors were identified as two major downstream effectors of Ptf1a. PMID: 25966682
    4. This study demonstrated that the transcription factor Ptf1a is capable of cell autonomously redirecting cortical pyramidal cell identity toward an inhibitory peptidergic fate. PMID: 25878276
    5. We provide strong evidence for spatiotemporally context-dependent activity of Ptf1a as a potent gain-of-function trigger of pro-pancreatic commitment. PMID: 25371369
    6. Data suggest the role of pancreas-specific transcription factor 1a (PTF1a) in pancreas development in directing differentiation of embryonic stem cells (ESCs) to endocrine cells. PMID: 24375815
    7. These data identify Ptf1a as the first gene involved in the segregation of the cerebellum from the more ventral brainstem. PMID: 24733890
    8. The results of this study suggested that Ptf1a specify spatial identities of cerebellar neuron progenitors in the neuroepithelium, leading to appropriate production of GABAergic and glutamatergic neurons, respectively. PMID: 24695699
    9. The results support a model whereby Ptf1a directs distinct gene expression programs that are established by the interplay of chromatin accessibility, collaborating transcription factors, and the DNA motifs within tissue-specific regulatory enhancers. PMID: 23754747
    10. and and V PMID: 23349836
    11. Ptf1a-deficient mice lack all inhibitory interneurons in the dorsal horn. PMID: 23616538
    12. we demonstrated that misexpression of Ptf1a, but not of Gm13344 or Gm13336, in the notochord, hindgut, cloaca, and mesonephros was sufficient to replicate the Sd phenotype PMID: 23436999
    13. We show that Ptf1a expression is significantly upregulated in Sd mutant embryos. PMID: 23437000
    14. The Sd heterozygote phenotype was not ameliorated by Ptf1a haploinsufficiency, further suggesting that the developmental defects result from ectopic expression of Ptf1a. PMID: 23437001
    15. binding of Rbms3 to the 3'UTR of Ptf1a regulates the production of the Ptf1a protein PMID: 22372950
    16. Tlx1/3 and Ptf1a, the key transcription factors for fate determination of glutamatergic and GABAergic neurons in the dorsal spinal cord PMID: 22723691
    17. Mnx1, a gene that is absolutely required in pancreas progenitors, is a major direct target of Ptf1a and is regulated by a distant enhancer element. PMID: 22232429
    18. miR-18a plays a fine-tuning role in regulating pancreatic progenitors and exocrine cells through the repression of Ptf1a expression. PMID: 22265691
    19. Ptf1a-induced Dll1 expression stimulates multipotent pancreatic progenitor cells proliferation and pancreatic growth by maintaining Hes1 expression and Ptf1a protein levels. PMID: 22096075
    20. Ptf1a, which plays a role in controlling both amacrine and horizontal cell fates, is a downstream effector of amyloid precursor protein (APP). PMID: 20978902
    21. Thus, cross-antagonism between Nkx6 and Ptf1a in multipotent progenitors governs the equilibrium between endocrine and acinar cell neogenesis required for normal pancreas development. PMID: 20627083
    22. Replacement of Rbpj by Rbpjl in the PTF1 complex drives acinar differentiation by maximizing secretory protein synthesis. PMID: 20398665
    23. Nephrin and Neph3 might regulate certain developmental aspects of Ptf1a-positive neurons as homo- or heterooligomers. PMID: 19887377
    24. evidence that Ptf1a expression is specifically connected to the acquisition of pancreatic fate by undifferentiated foregut endoderm PMID: 12185368
    25. Ptf1 function is inhibited by notch PMID: 15280211
    26. Ptf1a is involved in driving neural precursors to differentiate into GABAergic neurons in the cerebellum. PMID: 16039563
    27. Ptf1a function is essential for GABAergic over glutamatergic neuronal cell fates in the developing spinal cord, and provides an important genetic link between inhibitory and excitatory interneuron development. PMID: 16291784
    28. Ptf1a determines horizontal and amacrine cell fates during mouse retinal development. PMID: 17075007
    29. Ptf1a contributes to the differentiation of horizontal cells and types of amacrine cells during mouse retinogenesis. PMID: 17301087
    30. These findings indicate that Ptf1a is necessary for the specification and normal production of Purkinje cells and cerebellar interneurons. PMID: 17360405
    31. interaction between PTF1a and RBPJ is required for the early stage of pancreatic growth, morphogenesis, and lineage fate decisions PMID: 17938243
    32. The dosage of Ptf1a is crucial for pancreas specification, growth, total beta-cell number, islet morphogenesis, and endocrine function PMID: 18591390
    33. The trimeric PTF1 complex forms dual autoregulatory loops with the Ptf1a and Rbpjl genes that may maintain the stable phenotype of pancreatic acinar cells. PMID: 18606784
    34. Ptf1a is a master regulator, as indicated by a requirement of Ptf1a for the expression of GlyT2 and a set of peptides, including neuropeptide Y (NPY), nociceptin/orphanin FQ (N/OFQ), somatostatin (SOM), enkephalin (ENK), dynorphin (DYN) and galanin (GAL) PMID: 18634777
    35. inhibitory and excitatory subtypes of CN neurons are defined by Ptf1a and Atoh1, respectively and, furthermore, provide important insights into understanding the machinery of neuron subtype specification in the dorsal hindbrain. PMID: 19439493
    36. Data show that Neurog2 expression is directly regulated by the PTF1-Rbpj complex, identifying Neurog2 as the first neural target of Ptf1a and revealing a bHLH cascade in the specification of GABAergic neurons in the dorsal spinal cord and cerebellum. PMID: 19641016

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Note=In the cytoplasm loses its ability to form the PTF1 complex.
  • 组织特异性：
    Expressed in precursors of pancreatic islets, acini and ducts.
  • 数据库链接：

    KEGG: mmu:19213

    STRING: 10090.ENSMUSP00000028068

    UniGene: Mm.69647