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  4. Recombinant Mouse Paired box protein Pax-6 (Pax6)

Recombinant Mouse Paired box protein Pax-6 (Pax6)

  • 货号：
    CSB-YP017492MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP017492MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP017492MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP017492MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP017492MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Pax6; Pax-6; Sey; Paired box protein Pax-6; Oculorhombin
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-422
  • 氨基酸序列
    MQNSHSGVNQ LGGVFVNGRP LPDSTRQKIV ELAHSGARPC DISRILQVSN GCVSKILGRY YETGSIRPRA IGGSKPRVAT PEVVSKIAQY KRECPSIFAW EIRDRLLSEG VCTNDNIPSV SSINRVLRNL ASEKQQMGAD GMYDKLRMLN GQTGSWGTRP GWYPGTSVPG QPTQDGCQQQ EGGGENTNSI SSNGEDSDEA QMRLQLKRKL QRNRTSFTQE QIEALEKEFE RTHYPDVFAR ERLAAKIDLP EARIQVWFSN RRAKWRREEK LRNQRRQASN TPSHIPISSS FSTSVYQPIP QPTTPVSSFT SGSMLGRTDT ALTNTYSALP PMPSFTMANN LPMQPPVPSQ TSSYSCMLPT SPSVNGRSYD TYTPPHMQTH MNSQPMGTSG TTSTGLISPG VSVPVQVPGS EPDMSQYWPR LQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells. Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains. Acts as a transcriptional repressor of NFATC1-mediated gene expression.
  • 基因功能参考文献：
    1. Tgfbeta2, Bmp4 and Foxc1 are direct downstream targets of Pax6 in the developing iris. PMID: 27771509
    2. a novel regulatory relationship between the retinal pigmented epithelium (RPE) transcription factors Pax6 and Sox9 that controls the timing of RPE differentiation and the adjacent choroid maturation, is reported. PMID: 29986868
    3. overexpression of CHD1L in embryonic cells upregulated the expression of ectoderm genes, especially PAX6 PMID: 28946814
    4. Comparative functional analyses revealed that the neurogenic function of Pax6 is highly conserved in the developing mouse and chick pallium, whereas stage-specific binary functions of Pax6 in neurogenesis are unique to mouse neuronal progenitors, consistent with Pax6-dependent temporal regulation of Notch signaling. Pax6-dependent enhancer activity of Dbx1 is extensively conserved between mammals and chick. PMID: 29661783
    5. These results demonstrate a novel role for Tlx3 and indicate that Pax6-Tlx3 expression and interaction is part of a region specific regulatory network in cerebellum and its deregulation during development could possibly lead to Autistic spectral disorders (ASD). PMID: 27452274
    6. Pax6 (and IBA1) co-localize in the brain and also interact physically. PMID: 28476689
    7. Pax6 normally represses Cdca7 expression in the lateral cortex and that repression of Cdca7 in cells of this region is required for their production of a normal complement of Tbr2-expressing intermediate progenitors PMID: 28583079
    8. this Pax6 (fl) allele provides a useful addition to the existing Pax6 allelic series and this study demonstrates the utility of using compound heterozygotes with null alleles to unmask cryptic effects of floxed alleles. PMID: 27240603
    9. Data show that Pax6 acts in RPCs to control differentiation of multiple late-born neuronal cell types. PMID: 28993200
    10. reduced expression of PAX6 in metabolically stressed beta cells may contribute to beta cell failure and alpha cell dysfunction in diabetes. PMID: 27941241
    11. The expression of Barhl2 in the thalamus and pretectum is related to, and regulated by, the expression of Pax6. Pax6 is known to be required for normal diencephalic development. The findings suggest that some of its actions might be mediated by its maintenance of a repressive influence over Barhl2 expression in the thalamus and pretectum. PMID: 27887593
    12. The present study shows how Pax6 is key to the development of glutamatergic cells in the cerebellum PMID: 27581449
    13. These data suggest that Pax6 and Sox2 have counteracting roles in regulating neurogenesis. PMID: 27171428
    14. paternal aging has profound effects on the onset of behavioral abnormalities in mice carrying a mutation of Pax6, a gene with neurodevelopmental regulatory functions. PMID: 27855195
    15. HDAC1 binds PAX6 and protein-protein interaction leads to transcriptional repression of PAX6 target genes during mouse retinal development PMID: 27871855
    16. These results indicate that Pax6 is required for the functional identity of adult beta cells. PMID: 28377501
    17. Results showed that multiple sevoflurane exposure in pregnant mice caused the decrease of Pax6 and Ccnd1 expression, the inhibition of NSCs proliferation and fetal hippocampus neurogenesis PMID: 28279665
    18. RIP140 protects LSD1's catalytic domain and antagonizes its Jade-2-mediated ubiquitination and degradation. PMID: 26372689
    19. Glucose-stimulated expression of ALX3 in alpha cells provides a regulatory mechanism for the downregulation of glucagon gene expression by interfering with PAX6-mediated transactivation on the glucagon G1 promoter element. PMID: 26739814
    20. c.718C>T transitional mutation of PAX6 has been found to underlie the aniridia, which showed an autosomal dominant inheritance pattern in this northeastern Chinese family PMID: 27455012
    21. novel mutation in the PAX6 gene has been identified in a Chinese family affected with aniridia PMID: 27455013
    22. the Pax6 paired domain has roles in neural tube patterning and peripheral nervous system development PMID: 26187199
    23. This study demonstrated that Pax6 is expressed in astrocytes and binds to the GLT-1 promoter in vitro and in vivo. PMID: 26485579
    24. Pax6 inactivation in the adult pancreas reveals ghrelin as endocrine cell maturation marker. PMID: 26658466
    25. results indicate that Pax6 is required by thalamic progenitors for the normal molecular patterning of the thalamic neurons that they generate PMID: 26520399
    26. Pax6 expression is essential for horizontal basal cells to differentiate into neuronal cells during regeneration. PMID: 25808240
    27. Upper-layer neuron production was increased in double-transgenic mouse embryos with sustained Pax6 expression in the neurogenic lineage. Increased basal progenitors existed in the subventricular zone and the intermediate zone of the neocortex. PMID: 26252244
    28. The expression dynamics shows that Pax6 localizes at XY body during meiotic prophase I, especially in the mid-phase of pachytene spermatocytes which suggests that Pax6 may have some function in inactivation of sex chromosomes during meiosis. PMID: 26032914
    29. Study demonstrates that FGFR2 plays an essential role in controlling cell proliferation and differentiation, and maintaining Pax6 levels in corneal epithelium via ERK-independent pathways during embryonic development. PMID: 25615698
    30. PAX6 expression in the inner retina of calcitonin receptor-like receptor transgenic (CLR(SMalphaA)) mice suggest a role in regulatory mechanisms involved in glaucoma-related retinal cell death. PMID: 25827297
    31. Novel Pax6-dependent gene regulatory network that contribute to the function of Pax6 were identified in both forebrain and lens. PMID: 26138486
    32. PAX6 downregulates miR-124 expression to promote cell migration during embryonic stem cell differentiation PMID: 24773074
    33. Onecut1 and Onecut2 have roles in operating downstream of Pax6 to regulate horizontal cell development PMID: 25794677
    34. deregulation of the cell cycle machinery and TF expression in aOBSCs which are deficient in Pax6 may be at the origin of the phenotypes observed in this adult NSC population. PMID: 25117830
    35. Targets of RBPJ/N1ICD in cortical neural stem cell at a genome-wide level, were identified. PMID: 22232070
    36. Pax6 blocks diencephalic Shh expression cell autonomously. Absence of Pax6 causes non-cell-autonomous diencephalic patterning defects. PMID: 25176648
    37. Retinal pigment epithelium (RPE) of a beta-catenin/Pax6 double mutant was differentiated into the neural retina; however, the tissue was thinner than that of the conditional beta-catenin mutant due to reduced proliferation; data show that Pax6 is required for the RPE differentiation by regulating pigmentation and accountable for hyperproliferation in the transdifferentiated RPE. PMID: 25689933
    38. Data (including data from heterozygous Pax6 mutant mouse strain) suggest that Pax6 plays role in development of diabetes type 2 (DM2T); early-onset DM2T can result from interaction of genetic/environmental factors involving Pax6 and high-fat diet. PMID: 24925705
    39. The existence of spatial compartmentation in the rhombic lip and the interplay between Wls, Math1, and Pax6 in the rhombic lip provides novel views of early cerebellar development. PMID: 25209290
    40. Deletion of either Pax6 in the subependymal zone (SEZ) causes the progeny of adult neural stem cells to convert to the ependymal lineage within the SEZ while migrating neuroblasts convert to different glial lineages en route to or in the olfactory bulb PMID: 23933087
    41. Pax6 is a novel downstream target of the Wnt/ss-catenin pathway, and ss-catenin/Pax6 signaling plays critical roles in self-renewal and neurogenesis of radial glia/neural stem cells during neocortical development. PMID: 24115331
    42. Pax6 has an evolutionarily conserved function in establishing the temporospatial expression of Shh in the mid-diencephalic organizer in vertebrates. PMID: 24528677
    43. The zinc-finger protein Sp8 plays a supplementary role to Pax6 in establishing the pMN/p3 domain boundary. PMID: 24948600
    44. functional role of Pax6 loss in SQM pathogenesis PMID: 24143217
    45. PAX6, in addition to each patterning function, is expressed in a group of late born interneurons that derive from the p2 and p0 domains. PMID: 23816521
    46. Results of the present study suggest that Pax6 is expressed in bone and may play an important role in osteocyte differentiation by controlling canonical Wnt signaling. PMID: 23529217
    47. Increased corneal epithelial turnover contributes to abnormal homeostasis in the Pax6(+/-) mouse model of aniridia. PMID: 23967157
    48. when Pax6 was inactivated at the onset of contact between the optic vesicle and surface ectoderm in Pax6(Sey/flox) embryos, expression of lens-specific genes was not initiated and neither the lens nor the retina formed PMID: 24523460
    49. Manipulation of Pax6 dosage through a mutation-independent nonsense mutation suppression strategy limits progressive, postnatal damage in the eye. PMID: 24355924
    50. Mutant PAX6 binds the Pc2 promoter and regulates promoter activity. PMID: 24047795

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  • 相关疾病：
    Defects in Pax6 are the cause of a condition known as small eye (Sey) which results in the complete lack of eyes and nasal primordia.
  • 亚细胞定位：
    Nucleus.; [Isoform 1]: Nucleus.; [Isoform 3]: Nucleus. Cytoplasm.; [Isoform 5a]: Nucleus.
  • 蛋白家族：
    Paired homeobox family
  • 组织特异性：
    Expressed in osteoclasts.; [Isoform 1]: Dominant isoform expressed in the eye, including in the retina and cornea. Abundantly expressed in the lens epithelium.; [Isoform 3]: Dominant isoform expressed in the eye, including in the retina and cornea. Weakly
  • 数据库链接：

    KEGG: mmu:18508

    STRING: 10090.ENSMUSP00000087870

    UniGene: Mm.3608