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  4. Recombinant Mouse Nuclear receptor subfamily 6 group A member 1 (Nr6a1)

Recombinant Mouse Nuclear receptor subfamily 6 group A member 1 (Nr6a1)

  • 货号：
    CSB-YP720657MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP720657MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP720657MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP720657MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP720657MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Nr6a1; Gcnf; Nuclear receptor subfamily 6 group A member 1; Germ cell nuclear factor; GCNF; mGCNF; Retinoid receptor-related testis-specific receptor; RTR
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-495
  • 氨基酸序列
    MERDERPPSG GGGGGGSAGF LEPPAALPPP PRNGFCQDEL AELDPGTNGE TDSLTLGQGH IPVSVPDDRA EQRTCLICGD RATGLHYGII SCEGCKGFFK RSICNKRVYR CSRDKNCVMS RKQRNRCQYC RLLKCLQMGM NRKAIREDGM PGGRNKSIGP VQISEEEIER IMSGQEFEEE ANHWSNHGDS DHSSPGNRAS ESNQPSPGST LSSSRSVELN GFMAFRDQYM GMSVPPHYQY IPHLFSYSGH SPLLPPQARS LDPQSYSLIH QLMSAEDLEP LGTPMLIEDG YAVTQAELFA LLCRLADELL FRQIAWIKKL PFFCELSIKD YTCLLSSTWQ ELILLSSLTV YSKQIFGELA DVTAKYSPSD EELHRFSDEG MEVIERLIYL YHKFHQLKVS NEEYACMKAI NFLNQDIRGL TSASQLEQLN KRYWYICQDF TEYKYTHQPN RFPDLMMCLP EIRYIAGKMV NVPLEQLPLL FKVVLHSCKT STVKE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Orphan nuclear receptor. Binds to a response element containing the sequence 5'-TCAAGGTCA-3'. May be involved in the regulation of gene expression in germ cell development during gametogenesis.
  • 基因功能参考文献：
    1. Here, the s present the first structure of mouse GCNF DNA-binding domain in complex with the Oct4 DR0. The overall structure revealed two molecules bound in a head-to-tail fashion on opposite sides of the DNA at a DR0 motif located within the Oct4 promoter. PMID: 27984042
    2. Inactivation of the Gcnf gene in vivo leads to loss of repression of Oct4 expression in both male and female gonads. PMID: 25140725
    3. Gcnf is expressed in fetal ovarian germ cells. PMID: 23762465
    4. characterize the let-7 target Nr6a1, an embryonic transcriptional repressor that regulates gene expression in adult fibroblasts following miRNA loss PMID: 23630078
    5. GCNF initiates the repression and epigenetic modification of Oct4 gene during ESC differentiation. PMID: 21608077
    6. These experiments confirmed that NR6A1 works as a regulator for hypocretin transcription. PMID: 21056546
    7. Examination of RTR expression in murine placental tissue by immunohistochemistry demonstrated the presence of RTR protein in the nuclei of giant trophoblasts and spongiotrophoblasts. PMID: 11969338
    8. embryonic function is dependent on the DNA binding site PMID: 12381721
    9. GCNF in early embryos is a maternal protein and could be involved in the regulation of zygotic gene expression and preimplantation embryonic development. PMID: 12493724
    10. germ cell nuclear factor plays a role in transcriptional regulation during meiosis and the early haploid phase of spermatogenesis PMID: 12606326
    11. androgen-regulated gene expressions located at the corpus principal cells might be controlled by GCNF. PMID: 12639946
    12. GCNF-dependent repression of Bmp15 and Gdf9 mediates gamete regulation of female fertility. PMID: 12912906
    13. germ cell nuclear factor (GCNF) may play an important role in epididymal differentiation PMID: 15064830
    14. Germ cell nuclear factor acts as a repressor of basal transcription on both the Protamine 1 and Protamine 2 promoters. PMID: 15236322
    15. Expression of GCNF may play important roles in spermatogenesis, capacitation and fertilization. PMID: 15273870
    16. The level of GCNF is critical for differentiation and maturation of neuronal precursor cells. PMID: 15297607
    17. Three hypersensitive sites were identified in the region spanning 2.1kb between the last exon of GCNF and the first exon of Ad4BP/SF-1. PMID: 15737622
    18. GCNF has a central role in the repression of pluripotency gene expression during retinoic acid-induced embryonic stem cell differentiation PMID: 16166633
    19. Required for establishment of the isthmic organizer, thereby regulating the midbrain development. PMID: 16530751
    20. We found that GCNF interacts directly with Dnmt3 molecule(s) and verified that this interaction induces the methylation of the Oct-3/4 promoter. PMID: 16631596
    21. These results suggested that GCNF might be a negative regulator of PPARdelta gene expression through its direct interaction with PPARdelta promoter in mouse testis. PMID: 18330480
    22. Nr6a1 is expressed not only in the germ cells of mouse but also in a variety of somatic tissues, including epididymis, oviduct, brain, and pituitary. PMID: 19164181
    23. The suppression of Oct4 by GCNF is important for the transition from primitive to definitive neural stem cells and restriction of the non-neural competency in the early neural stem cell lineage. PMID: 19228964

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Nuclear hormone receptor family, NR6 subfamily
  • 组织特异性：
    Expressed in the germ cells of both the adult testis and ovary, being most abundant in spermatids.
  • 数据库链接：

    KEGG: mmu:14536

    STRING: 10090.ENSMUSP00000075624

    UniGene: Mm.439703