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  4. Recombinant Mouse Nuclear receptor subfamily 4 group A member 1 (Nr4a1)

Recombinant Mouse Nuclear receptor subfamily 4 group A member 1 (Nr4a1)

  • 货号：
    CSB-YP016062MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP016062MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP016062MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP016062MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP016062MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Nr4a1; Gfrp; Hmr; N10; Nur77; Nuclear receptor subfamily 4 group A member 1; Nuclear hormone receptor NUR/77; Nuclear protein N10; Orphan nuclear receptor HMR
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-601
  • 氨基酸序列
    MPCIQAQYGT PATSPGPRDH LTGDPLALEF GKPTMDLASP ETAPAAPATL PSFSTFMDGY TGEFDTFLYQ LPGTTQPCSS ACSSASSTSS SSSSATSPAS ASFKFEDFQV YGCYPGTLSG PLDETLSSSG SEYYGSPCSA PSPSTPNFQP SQLSPWDGSF GHFSPSQTYE GLWAWTEQLP KASSGPPPPP TFFSFSPPTG PSPSLAQSSL KLFPPPATHQ LGEGESYSMP AAFPGLAPTS PNRDTSGILD APVTSTKSRS GASGGSEGRC AVCGDNASCQ HYGVRTCEGC KGFFKRTVQK SAKYICLANK DCPVDKRRRN RCQFCRFQKC LAVGMVKEVV RTDSLKGRRG RLPSKPKQPP DASPTNLLTS LIRAHLDSGP STAKLDYSKF QELVLPRFGK EDAGDVQQFY DLLSGSLDVI RKWAEKIPGF IELCPGDQDL LLESAFLELF ILRLAYRSKP GEGKLIFCSG LVLHQLQCAR GFGDWIDNIL AFSRSLHSLG VDVPAFACLS ALVLITDRHG LQDPRRVEEL QNRIASCLKE HMATVAGDPQ PASCLSRLLG KLPELRTLCT QGLQRIFCLK LEDLVPPPPI VDKIFMDTLS F
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3'. May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation. Plays a role in the vascular response to injury.
  • 基因功能参考文献：
    1. By facilitating Mff-mediated mitochondrial fission and FUNDC1-required mitophagy, NR4A1 disturbed mitochondrial homeostasis, enhanced endothelial apoptosis and provoked microvascular dysfunction in myocardial ischemia reperfusion injury. PMID: 29744594
    2. identify Nur77 as a transcriptional regulator of T cell metabolism, which elevates the threshold for T cell activation and confers protection in different T cell-mediated inflammatory diseases. PMID: 30072431
    3. The data show that NR4A1 expression by multipotent progenitors of the spleen limits erythropoiesis and megakaryopoeisis, permitting development to other myeloid lineages. This effect is specific to the spleen, revealing a unique molecular pathway that regulates myeloid bias in an extramedullary niche. PMID: 29282309
    4. NR4A1 knockdown interrupted the Mff-related mitochondrial fission and recused Parkin-mediated mitophagy, reducing the hyperglycemia-mediated mitochondrial damage and thus improving renal function. PMID: 30077998
    5. we found that either knockdown of Nr4a1 or 3, 3'- Diindolylmethane (DIM), an Nr4a1 antagonist, were able to rescue the effects of SNRPN knockdown on neurite outgrowth of embryonic cortical neurons, providing the potential therapeutic methods for SNRPN deletion disorders. We thus concluded that maintaining the proper level of SNRPN is critical in cortical neurodevelopment. PMID: 27430727
    6. new insights into the understanding the mechanisms of the effects of Nur77 on LPS-activated microglia PMID: 28729074
    7. Data show that SUMOylation is critical in controlling NR4A1 function in inflammatory cytokine signaling and controlling macrophage cell death. PMID: 28622293
    8. our data demonstrated that NR4A1 protein physically associates with the WT1 promoter, and enhanced WT1 promoter transactivation and knockdown of WT1 in MIN6 cells induced apoptosis. These findings suggest that NR4A1 protects pancreatic beta-cells against H2O2 mediated apoptosis by up-regulating WT1 expression. PMID: 28342843
    9. These results suggest that berberine-induced activation of AMPK may contribute to hepatic FGF21 expression via NUR77. PMID: 29247651
    10. we found that KLF6 transcriptionally cooperates with NUR77 and SF1 PMID: 26874000
    11. additional loss of Nur77 in the absence of Bim led to diabetes induction, suggesting that Nur77 promotes tolerance in this context. Together, these data reveal novel nondeletional roles for Nur77 that differ between T cell subsets and have implications for self-tolerance. PMID: 28947542
    12. current data identify Nr4a1 as regulator of microglia activation and potentially new target for the treatment of inflammatory CNS diseases such as multiple sclerosis. PMID: 28411187
    13. Celastrol promotes Nur77 migration from the nucleus to mitochondria, where it is ubiquitinated by TRAF2. Ubiquitinated Nur77 then interacts with p62/SQSTM1, leading to autophagy of dysfunctional mitochondria and alleviation of inflammation. PMID: 28388439
    14. New transcript variants encode for NUR77 protein isoforms which are significantly smaller in size due to lack of transactivation domain and a part of DNA binding domain. Western blot analysis using NUR77 specific antibody confirmed the existence of these smaller variants in mouse. PMID: 28111880
    15. Nur77 deficiency compromises myofiber growth, but not the regenerative capacity of myogenic progenitor cells. PMID: 28170423
    16. Using Nr4a1 transgenic mice, electrically evoked dopamine release was shown to differ between the striosome and matrix compartments of the corpus striatum in a regionally-distinct manner. PMID: 27036891
    17. Nur77 was found to be an important modulator in microglia function and attenuated pro-inflammatory mediator production. PMID: 27889907
    18. this study shows that in NR4A1-/- mice, inflammatory Ly6Chigh monocytes contribute to rapid development of arthritis in a serum transfer-induced arthritis model PMID: 27600773
    19. Nur77 downregulation triggers pulmonary artery smooth muscle cell proliferation and migration in mice with hypoxic pulmonary hypertension PMID: 27871853
    20. Our findings may throw light on the function of ApoA4 in inflammatory responses and acute-phase reactions, as well as the development of SERPINA3 relative diseases. PMID: 28412351
    21. This study demonstrated that NGFI-B plays a role in controlling correct cerebellar development. PMID: 27769911
    22. Nur77 modulates the inflammatory state of macrophages by decreasing inflammatory gene expression, inhibits the collagen content of the extracellular matrix of macrophages, and reduces phagocytosis by macrophages. PMID: 26932821
    23. Detailed knowledge on the role of Nur77 in maintaining cardiomyocyte Ca(2+) homeostasis and the dual role Nur77 plays in cardiac remodelling will aid in developing personalized therapies against heart failure PMID: 26486271
    24. Nuclear receptors of the NR4a family are not required for the development and function of follicular T helper cells PMID: 25899083
    25. NR4A1 functions as a master transcription factor for the development of this novel thymus-specific macrophage subset. PMID: 26091486
    26. Identified Nur77/Nor1 as novel regulators of thrombomodulin expression and function in vascular endothelial cells. PMID: 26634653
    27. In both mouse colitis models inflammation was increased in Nur77-/- mice.Overexpression of Nur77 in lipopolysaccharide activated RAW macrophages up-regulated IL-10 and downregulated TNFalpha, MIF-1 and MCP-1 mRNA expression through NFkappaB repression. PMID: 26241646
    28. Nr4a1 factor plays crucial role regulatory T cells, mediating the two defining characteristics of regulatory T cells: stability of cell lineage and immunosuppressive functions. PMID: 26304965
    29. Data show that transcription factor Nr4a1 expression in myeloid cells limits experimental autoimmune encephalomyelitis (EAE) severity. PMID: 26523867
    30. Nur77 is a critical negative regulator of Ang II-induced VSMC proliferation, migration, and phenotypic switching via the downregulation of beta-catenin activity PMID: 26597820
    31. Mechanistically, NFATc1 induces Nur77 expression at late stage of osteoclast differentiation; in turn, Nur77 transcriptionally up-regulates E3 ubiquitin ligase Cbl-b, which triggers NFATc1 protein degradation. PMID: 26173181
    32. Nr4a1 induction is dependent on ERK1/2 and that MKP-1 negatively regulates this induction. PMID: 25657047
    33. Pgc-1alpha and Nr4a1 represent candidate genes for linking circadian neurohormone release with functional adaptation and healthiness of retina and photoreceptor cells. PMID: 26393668
    34. data support a novel and critical role for Nr4a1 in the regulation of CD8(+) T cell expansion and effector function through transcriptional repression of Irf4. PMID: 26363057
    35. Whereas NR4A1, induced by PDGF-BB, suppresses cell growth on a solid surface, it increases anchorage-independent growth. PMID: 25269081
    36. Nuclear receptor NR4A1 promotes breast cancer invasion and metastasis by activating TGF-beta signalling PMID: 24584437
    37. these findings support a protective role of Nur77 in OVA-induced airway inflammation and identify Nur77 as a novel therapeutic target for airway inflammation. PMID: 26170382
    38. These data show that GFP-Nur77 inhibited differentiation of oxLDL-treated macrophages into DC. T PMID: 25471687
    39. Results show that NR4A1 expression identifies a highly quiescent and distinct population of myeloid-biased LT-HSCs. PMID: 25284014
    40. Retinoids induce a Nur77-dependent cell death program in thymocytes activating the mitochondrial pathway of apoptosis. PMID: 25576519
    41. Nur77 appears essential for regulating early signaling of liver regeneration by modulating cytokine-mediated inflammatory, apoptotic, and energy mobilization processes PMID: 25307349
    42. These observations imply that zearalenone activity interferes with testosterone biosynthesis in mouse Leydig cells via the crosstalk of estrogen receptor signaling and Nur77 expression. PMID: 24512812
    43. Nur77 mediates its effects on muscle size by orchestrating transcriptional programs that favor muscle growth, including the induction of insulin-like growth factor 1 (IGF1), as well as downregulation of growth-inhibitory genes, Fbxo32 and Trim63. PMID: 25605333
    44. these results suggest that TGF-beta1/ALK5/Smad3 signaling represses the expression of steroidogenic genes via the suppression of Nur77 transactivation in testicular Leydig cells. PMID: 25140527
    45. Ectopic induction of Nur77 in endothelial cells is sufficient to improve skin wound healing. Furthermore, TR3/Nur77 regulates the expression of integrin beta4 by targeting its promoter activity. PMID: 25326539
    46. A role for endogenous MEF2 factors exclusively in hormone/Fsk/cAMP-induced Nr4a1 gene expression in mouse MA-10 Leydig cells. PMID: 24694307
    47. This study showed that nurr77 has a similar mRNA expression pattern and low levels of expression at rest. PMID: 24753204
    48. Data indicate that Nur77 is an important nuclear hormone receptor in regulation of atherosclerotic plaque formation and represents a promising target for the treatment of atherosclerosis. PMID: 24498071
    49. Fasting-induced activation of orphan nuclear receptor Nur77 increases hepatic fibroblast growth factor (FGF)21 expression. PMID: 24885573
    50. Accompanied by the substantial decline in apoptosis inducer Nur77, myocardial ischemia/reperfusion induced cardiac dysfunction was manifested as cardiomyopathy and increased Reactive Oxygen Spieces. PMID: 24680679

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Mitochondrion.
  • 蛋白家族：
    Nuclear hormone receptor family, NR4 subfamily
  • 组织特异性：
    Highest levels occur in the testis followed by brain and muscle tissue.
  • 数据库链接：

    KEGG: mmu:15370

    STRING: 10090.ENSMUSP00000023779

    UniGene: Mm.119