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  4. Recombinant Mouse Nuclear receptor subfamily 2 group E member 1 (Nr2e1)

Recombinant Mouse Nuclear receptor subfamily 2 group E member 1 (Nr2e1)

  • 中文名称：
    小鼠Nr2e1重组蛋白
  • 货号：
    CSB-YP737334MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Nr2e1重组蛋白
  • 货号：
    CSB-EP737334MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Nr2e1重组蛋白
  • 货号：
    CSB-EP737334MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Nr2e1重组蛋白
  • 货号：
    CSB-BP737334MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Nr2e1重组蛋白
  • 货号：
    CSB-MP737334MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Nr2e1
  • Uniprot No.：
  • 别名：
    Nr2e1; Tll; Tlx; Nuclear receptor subfamily 2 group E member 1; Nuclear receptor TLX; Protein tailless homolog; Tll; mTll
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-385
  • 氨基酸序列
    MSKPAGSTSR ILDIPCKVCG DRSSGKHYGV YACDGCSGFF KRSIRRNRTY VCKSGNQGGC PVDKTHRNQC RACRLKKCLE VNMNKDAVQH ERGPRTSTIR KQVALYFRGH KEDNGAAAHF PSTALPAPAF FTAVTQLEPH GLELAAVSAT PERQTLVSLA QPTPKYPHEV NGTPMYLYEV ATESVCESAA RLLFMSIKWA KSVPAFSTLS LQDQLMLLED AWRELFVLGI AQWAIPVDAN TLLAVSGMNT DNTDSQKLNK IISEIQALQE VVARFRQLRL DATEFACLKC IVTFKAVPTH SGSELRSFRN AAAIAALQDE AQLTLNSYIH TRYPTQPCRF GKLLLLLPAL RSISPSTIEE VFFKKTIGNV PITRLLSDMY KSSDI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Orphan receptor that binds DNA as a monomer to hormone response elements (HRE) containing an extended core motif half-site sequence 5'-AAGGTCA-3' in which the 5' flanking nucleotides participate in determining receptor specificity. Regulates cell cycle progression in neural stem cells of rhe developing brain. Involved in the regulation of retinal development and essential for vision. During retinogenesis, regulates PTEN-Cyclin D expression via binding to the promoter region of PTEN and suppressing its activity. May be involved in retinoic acid receptor (RAR) regulation in retinal cells.
  • 基因功能参考文献：
    1. Results demonstrate that social isolation stress during adolescence attenuates an exercise-induced increase in neurogenesis. This effect is most pronounced in the ventral hippocampus. TLX is necessary for the pro-neurogenic effects of exercise during adolescence. PMID: 28972669
    2. This study demonstrated that deletion of Tlx impairs motor, cognitive and anxiety-related behaviours during adolescence and adulthood in male and female mice with most effects occurring during adolescence rather than adulthood, independent of housing conditions. PMID: 26970576
    3. This study demonstrated that Nr2e1 could be regulated by retinoic acid, which would aid a better understanding of the mechanism underlying RA-induced brain abnormality. PMID: 28427488
    4. Nr2e1 deficiency augments palmitate-induced oxidative stress. Nr2e1 deficiency also resulted in decreases in antioxidant enzymes and expression level of Nrf2. PMID: 26649147
    5. TLX is essential for SOX2-mediated in vivo reprogramming of astrocytes and itself is also sufficient to induce neurogenesis in the adult striatum. PMID: 26607952
    6. TLX, an essential regulator of NSC proliferation and self-renewal, inhibits miR-219 processing. miR-219 suppresses mouse NSC proliferation downstream of TLX. PMID: 26965827
    7. At high glucose concentrations in vitro, AdipoR1 regulated the survival of neural stem cells through the p53/p21 pathway and the proliferation- and differentiation-related factors of neural stem cells via TLX. PMID: 26247729
    8. role in regulating STAT1 signaling and host defense PMID: 26196739
    9. Nr2e1 regulates processes involved in neurite development and terminal retinal cell differentiation. PMID: 26092486
    10. we have provided new candidate interacting partners and numerous well-developed testable hypotheses for understanding the pathways by which Nr2e1 functions to regulate neocortex development. PMID: 26204903
    11. TLX overexpression promotes beta cell proliferation and decreases cell apoptosis causes significant expression changes of 225 genes. PMID: 26471300
    12. data suggest that miR-378 is a novel miRNA that regulates NSC proliferation and differentiation via targeting TLX PMID: 26361139
    13. NR2E1 regulates CBX7 and restrains senescence in neural stem cells. PMID: 25328137
    14. TLX functions as a potent suppressor of oncogene-induced senescence in prostate cancer via its transcriptional co-regulation of the CDKN1A (p21(WAF1) (/) (CIP1) ) and SIRT1 genes PMID: 25557355
    15. MiR-9 and TLX form a feedback regulatory loop to coordinate the proliferation. PMID: 24901604
    16. These results suggest a strong association between hippocampal neurogenesis and cognition, as well as significant contributions of TLX to hippocampal neurogenesis, learning, and memory. PMID: 24927526
    17. Results of the current study suggest aggression displayed by TLX null and heterozygous mice involves 5-HT(2A/C) receptors. PMID: 23933143
    18. By interacting with its cofactors, Tlx represses its target genes and plays an important role in the maintenance of adult neural stem cells. (Review) PMID: 23415832
    19. TLX/NR2E1 and related NRs such as PNR and COUPTFs can selectively associate with the developmental corepressor BCL11A via a conserved motif F/YSXXLXXL/Y within the RID1 and RID2 domains . The interaction with BCL11A facilitates TLX-mediated repression of the RARb2 gene. PMID: 23975195
    20. Interaction of NSD1 with the NR2E/F subfamily including COUP-TFI, COUP-TFII, EAR2 and TLX requires an F/YSXXLXXL/Y motif. Interactions of NSD1 with liganded NRs require an overlapping LXXLL motif. PMID: 23975195
    21. Characterization of TLX expression in neural stem cells and progenitor cells in adult mouse brains PMID: 22952666
    22. While brain tumors still form in Tlx mutant mice, these tumors are less infiltrative and rarely associate with the adult neurogenic niches, suggesting a non-stem-cell origin. PMID: 23028043
    23. miR-137 forms a feedback regulatory loop with TLX and LSD1 to control the dynamics between neural stem cell proliferation and differentiation during neural development. PMID: 22068596
    24. Cerebrum and olfactory bulb hypoplasia, hallmarks of the Nr2e1-null mice phenotype, were not fully corrected in animals harboring one functional copy of human NR2E1. PMID: 22290436
    25. Tlx affects gene expression in a positive and negative manner in in the neonatal activated neural stem cells and transit-amplifying progenitors. PMID: 21714038
    26. Nuclear orphan receptor TLX induces Oct-3/4 for the survival and maintenance of adult hippocampal progenitors upon hypoxia. PMID: 21135096
    27. our results do not support Nr2e1(frc/frc) mice as pharmacological models for bipolar disorder PMID: 20497236
    28. nuclear receptor tailless (Tlx), which in the adult is expressed exclusively in astrocyte-like B cells of the subventricular zone, acts as a key regulator of neural stem cell (NSC) expansion and brain tumor initiation from NSCs PMID: 20360385
    29. Introducing an expression vector of Tlx or cyclin D1 that lacks the let-7b recognition site rescued let-7b-induced proliferation deficiency, suggesting that TLX and cyclin D1 are targets for let-7b-mediated regulation of neural stem cell proliferation. PMID: 20133835
    30. compared sequence from human, mouse, and the puffer fish Fugu rubripes PMID: 12079282
    31. Loss of the tailless gene affects forebrain development and emotional behavior PMID: 12527005
    32. Expression of the transcription factor, tailless (tlx), is required for formation of superficial cortical layers. PMID: 12902391
    33. TLX maintains adult neural stem cells in an undifferentiated, proliferative state; TLX-expressing cells from adult brains can proliferate, self-renew and differentiate into all neural cell types in vitro PMID: 14702088
    34. Tlx underlies a fundamental developmental program of retinal organization and controls the generation of the proper numbers of retinal progenies and development of glial cells during the protracted period of retinogenesis. PMID: 15371513
    35. These studies indicate that tlx is an essential intrinsic regulator in the decision to proliferate or differentiate in the developing forebrain. PMID: 15385616
    36. Absence of the transcription factor tailless (tlx) leads to premature laminar development and thinning of neocortex. PMID: 15950290
    37. Transgenic human NR2E1 was sufficient to fix structural brain & eye abnormalities & hyperaggressiveness in mice deleted for mouse Nr2e1. NR2E1 protein & regulatory sequences are comparable between these species. PMID: 16000615
    38. Nr2e1 deletion can significantly alter both synaptic plasticity and dendritic structure in the dentate gyrus PMID: 16289828
    39. Data show that in the postnatal mouse retina, the orphan nuclear receptor tailless is strongly expressed in the proangiogenic astrocytes, which secrete VEGF and fibronectin. PMID: 16424942
    40. TLX interacts with atrophin1 (Atn1), a corepressor that is involved in human neurodegenerative dentatorubral-pallidoluysian atrophy (DRPLA) and that is essential for development of multiple tissues. PMID: 16702404
    41. The results indicate that TLX plays an important role in neural development by regulating cell cycle progression and exit of neural stem cells in the developing brain. PMID: 17901127
    42. In the tailless germline mutants, They are less anxious and much more aggressive than controls, In contrast to germline mutants, the conditional mutants are not impaired in fear conditioning. PMID: 17953618
    43. TLX (also called NR2E1) regulates adult neural stem cell proliferation in a cell-autonomous manner by controlling a defined genetic network implicated in cell proliferation and growth PMID: 18235445
    44. findings demonstrate the crucial role of Tlx in the generation and maintenance of neural stem cells in the adult subventricular zone in vivo PMID: 18794344
    45. Here the s show that miR-9 suppresses TLX expression to negatively regulate neural stem cell proliferation and accelerate neural differentiation. PMID: 19330006

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Nuclear hormone receptor family, NR2 subfamily
  • 组织特异性：
    Expressed in embryonic developing forebrain and in dorsal midbrain. Outside the neural epithelium only found in the surface ectoderm at the site of nasal placode formation, where subsequently the nasal and olfactory epithelium is formed. Also expressed in
  • 数据库链接：

    KEGG: mmu:21907

    STRING: 10090.ENSMUSP00000019938

    UniGene: Mm.287100