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  4. Recombinant Mouse Nuclear receptor ROR-alpha (Rora)

Recombinant Mouse Nuclear receptor ROR-alpha (Rora)

  • 货号：
    CSB-YP020069MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP020069MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP020069MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP020069MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP020069MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Rora; Nr1f1; Rzra; Nuclear receptor ROR-alpha; Nuclear receptor RZR-alpha; Nuclear receptor subfamily 1 group F member 1; RAR-related orphan receptor A; Retinoid-related orphan receptor-alpha
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-523
  • 氨基酸序列
    MESAPAAPDP AASEPGSSGS EAAAGSRETP LTQDTGRKSE APGAGRRQSY ASSSRGISVT KKTHTSQIEI IPCKICGDKS SGIHYGVITC EGCKGFFRRS QQSNATYSCP RQKNCLIDRT SRNRCQHCRL QKCLAVGMSR DAVKFGRMSK KQRDSLYAEV QKHRMQQQQR DHQQQPGEAE PLTPTYNISA NGLTELHDDL STYMDGHTPE GSKADSAVSS FYLDIQPSPD QSGLDINGIK PEPICDYTPA SGFFPYCSFT NGETSPTVSM AELEHLAQNI SKSHLETCQY LREELQQITW QTFLQEEIEN YQNKQREVMW QLCAIKITEA IQYVVEFAKR IDGFMELCQN DQIVLLKAGS LEVVFIRMCR AFDSQNNTVY FDGKYASPDV FKSLGCEDFI SFVFEFGKSL CSMHLTEDEI ALFSAFVLMS ADRSWLQEKV KIEKLQQKIQ LALQHVLQKN HREDGILTKL ICKVSTLRAL CGRHTEKLMA FKAIYPDIVR LHFPPLYKEL FTSEFEPAMQ IDG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Nuclear receptor that binds DNA as a monomer to ROR response elements (RORE) containing a single core motif half-site 5'-AGGTCA-3' preceded by a short A-T-rich sequence. Key regulator of embryonic development, cellular differentiation, immunity, circadian rhythm as well as lipid, steroid, xenobiotics and glucose metabolism. Considered to have intrinsic transcriptional activity, have some natural ligands like oxysterols that act as agonists (25-hydroxycholesterol) or inverse agonists (7-oxygenated sterols), enhancing or repressing the transcriptional activity, respectively. Recruits distinct combinations of cofactors to target genes regulatory regions to modulate their transcriptional expression, depending on the tissue, time and promoter contexts. Regulates genes involved in photoreceptor development including OPN1SW, OPN1SM and ARR3 and skeletal muscle development with MYOD1. Required for proper cerebellum development, regulates SHH gene expression, among others, to induce granule cells proliferation as well as expression of genes involved in calcium-mediated signal transduction. Regulates the circadian expression of several clock genes, including CLOCK, ARNTL/BMAL1, NPAS2 and CRY1. Competes with NR1D1 for binding to their shared DNA response element on some clock genes such as ARNTL/BMAL1, CRY1 and NR1D1 itself, resulting in NR1D1-mediated repression or RORA-mediated activation of clock genes expression, leading to the circadian pattern of clock genes expression. Therefore influences the period length and stability of the clock. Regulates genes involved in lipid metabolism such as apolipoproteins APOA1, APOA5, APOC3 and PPARG. In liver, has specific and redundant functions with RORC as positive or negative modulator of expression of genes encoding phase I and phase II proteins involved in the metabolism of lipids, steroids and xenobiotics, such as CYP7B1 and SULT2A1. Induces a rhythmic expression of some of these genes. In addition, interplays functionally with NR1H2 and NR1H3 for the regulation of genes involved in cholesterol metabolism. Also involved in the regulation of hepatic glucose metabolism through the modulation of G6PC1 and PCK1. In adipose tissue, plays a role as negative regulator of adipocyte differentiation, probably acting through dual mechanisms. May suppress CEBPB-dependent adipogenesis through direct interaction and PPARG-dependent adipogenesis through competition for DNA-binding. Downstream of IL6 and TGFB and synergistically with RORC isoform 2, is implicated in the lineage specification of uncommitted CD4(+) T-helper (T(H)) cells into T(H)17 cells, antagonizing the T(H)1 program. Probably regulates IL17 and IL17F expression on T(H) by binding to the essential enhancer conserved non-coding sequence 2 (CNS2) in the IL17-IL17F locus. Involved in hypoxia signaling by interacting with and activating the transcriptional activity of HIF1A. May inhibit cell growth in response to cellular stress. May exert an anti-inflammatory role by inducing CHUK expression and inhibiting NF-kappa-B signaling.
  • 基因功能参考文献：
    1. We have identified RORalpha as a regulator of Treg genes responsible for suppressing allergic skin inflammation and also documented higher expression of RORalpha in skin-resident Tregs than in peripheral blood circulating Tregs in humans, suggesting that RORalpha and the TL1A-DR3 circuit could be therapeutically targeted in atopic dermatitis. PMID: 29500225
    2. These findings demonstrated for the first time that nuclear receptor RORalpha deficiency aggravated HFD-induced myocardial dysfunction at least in part by impairing mitochondrial biogenesis in association with disrupting AMPK-PGC1alpha signaling. PMID: 27825849
    3. RORalpha/gamma controls circadian expression of Insig2, which keeps feeding-induced SREBP1c activation under check PMID: 28747429
    4. RORalpha induces KLF4 mediated Kupffer cells polarization and protects against NASH. PMID: 28683306
    5. Data demonstrated that the circadian rhythm of testosterone synthesis in TM3 cells was disturbed following Fen treatment as evidenced by changes in the circadian rhythmicity of core clock genes (Bmal1, Rev-erbalpha, Roralpha). PMID: 29040059
    6. RORalpha inhibits PPARgamma-mediated transcriptional activity by interacting with HDAC3 and competing for the promoters of lipogenic genes. PMID: 28757615
    7. The expression RORalpha is significantly elevated under hypoxic conditions in keratinocytes in an HIF-1alpha dependent manner. PMID: 28332183
    8. that retinoic acid receptor-related orphan receptor alpha is a novel transcriptional regulator of SEMA3E-mediated neurovascular coupling in pathological retinal angiogenesis PMID: 28646017
    9. The deficiency of LXRalpha decreased glucose uptake after MI, resulting in a metabolic shift that suppressed glucose metabolism, which was in association with adverse cardiac remodeling. PMID: 28511797
    10. Data suggest that inflammatory response in macrophages, white adipocytes, and white adipose tissue in obese mice, includes up-regulation of expression of Rora; in adipose tissue, overexpression of Rora or treatment with Rora agonist enhances expression of cytokines, promotes macrophage infiltration, up-regulates expression of genes involved in ER stress response, and enhances signaling seen in unfolded protein response. PMID: 28698385
    11. these findings reveal that RORalpha regulates macrophage M2 polarization via activation of AMPKalpha PMID: 27788394
    12. RORalpha-dependent type 2 innate lymphoid cells are required and sufficient for type 2 cytokine expression and mucous metaplasia in immature mice. PMID: 28360114
    13. pharmacological activation of RORalpha by melatonin and SR1078 (a synthetic agonist) showed beneficial effects against diabetic cardiomyopathy, while the RORalpha inhibitor SR3335 significantly exacerbated cardiac impairments in diabetic mice. PMID: 27862268
    14. study provides the first direct evidence that the nuclear melatonin receptor RORalpha is a novel endogenous protective receptor against MI/R injury and an important mediator of melatonin-exerted cardioprotection; melatonin-RORalpha axis signaling thus appears important in protection against ischemic heart injury. PMID: 26797926
    15. RORalpha regulates pathologic retinal angiogenesis by modulating SOCS3-dependent inflammation PMID: 26243880
    16. This study showed that spontaneous Rora mutations causing cerebellar pathology are impaired in motor functions during the neonatal period. PMID: 25907855
    17. the inhibition of NF-kappaB by melatonin, but not that of NLRP3, was blunted in RORalpha (sg/sg) mice, indicating that functional RORalpha transcription factor is necessary for the initiation of the innate immune response against inflammation. PMID: 26045547
    18. Identify RORalpha as being essential to drive inflammation in experimental epidermolysis bullosa acquisita. PMID: 25953430
    19. The physiological function of RORalpha in regulating both glucose and free fatty acids homeostasis. PMID: 26015436
    20. Rora induces the mRNA level of antioxidant enzymes, superoxide dismutase 2 and glutathione peroxidase 1, through the Rora response elements located in the upstream promoters of Sod2 and Gpx1. PMID: 24597775
    21. The nuclear receptors retinoic acid receptor-related orphan receptors-alpha and gammat (RORalpha and RORgammat) play critical roles in the development of TH17 cells. PMID: 25560829
    22. Corl2/Skor2 has a role in regulating cerebellar Purkinje cell differentiation in a pathway involving RORalpha PMID: 24491816
    23. RORalpha is a key regulator of diurnal rhythm and fasting induction of CYP8B1, which regulates bile acid composition and serum and liver cholesterol levels. PMID: 24226095
    24. late postnatal expression of RORalpha cell-autonomously regulates the maintenance of Purkinje cell dendritic complexity PMID: 23719821
    25. This study suggests an autocrine Wnt5a-Ror signaling pathway that directs sympathetic axon branching during target innervation. PMID: 23454479
    26. Disruption of Roralpha1 and cholesterol 25-hydroxylase expression attenuates phagocytosis in male Roralphasg/sg mice. PMID: 23239817
    27. Data indicate that RORalpha-deficient, but not RORgammat-deficient, mice lacked natural helper (NH) cells in all tissues. PMID: 22981535
    28. RORalpha suppresses adipogenic differentiation at a later stage of differentiation by RORE-mediated stimulation of Dec1 and Dec2 expression. PMID: 22244086
    29. Rora expression was induced by cigarette smoke in mice and cell culture. Rora-deficient mice were protected from smoke induced airspace enlargement. DNA damage may contribute to pathogenesis of emphysema and Rora has a role in responses to genotoxicity PMID: 22744720
    30. RORalpha regulates survival and BCR expression in IgA+ memory B cells. PMID: 22561605
    31. Recent studies have expanded the role of RORalpha to other structures and functions in the brain. RORalpha was considered to be exclusively expressed in neurons in the brain--{REVIEW} PMID: 22223133
    32. Retinoid acid receptor-related orphan receptor (ROR)alpha's transcriptional dysregulation is found in the R6/2 mouse model for Huntington's Disease and in a model for spinocerebellar ataxia type 1 disease. PMID: 22712074
    33. transcription factor RORalpha was critical for the development of nuocytes and their role in the expulsion of parasitic worms PMID: 22267218
    34. RORalpha plays a critical role in the regulation of several aspects of metabolic syndrome. PMID: 21540300
    35. cholesterol signalling through increased Ror-alpha expression stimulates chondrocyte hypertrophy and partially mediates responses of cartilage to actin dynamics PMID: 20196782
    36. The neuroprotective function of RORalpha in astrocytes correlates with a downregulation of HIF-1alpha selectively in these cells. PMID: 21976517
    37. RIP140 acts as a coactivator for RORalpha, influencing clock gene expression. PMID: 21628546
    38. Study propose that Roralpha plays an important role in regulation of the AKT2 signalling cascade, which controls glucose uptake in skeletal muscle. PMID: 21279323
    39. Accelerated progression of the early steps of dendritic differentiation in culture involves increased expression of RORalpha in both Purkinje cells and interneurons. PMID: 20663205
    40. data identify new roles for ROR-alpha in molecular layer interneurons and radial glia development. PMID: 20722722
    41. RORalpha and RORgamma are ligand-regulated members of the NR superfamily and may serve as sensors for 7-oxygenated sterols. PMID: 19965867
    42. RORa regulates prosaposin which is involved in glycosphingolipid catabolism PMID: 11879571
    43. expression of ROR alpha(1) proteins in Colon 38 adenocarcinoma cells supports the concept that this receptor is involved in the oncostatic action of melatonin. PMID: 12019353
    44. ROR alpha acts through recruitment of gene-specific sets of transcriptional cofactors as part of the developmental program of ROR alpha-dependent gene expression in cerebellar development. PMID: 14687547
    45. Review. RORalpha, whose transcriptional activity is activated by cholesterol, is increases apoA-I expression, decreasing intracellular cholesterol content. Negative feedback regulation of intracellular cholesterol content is a new role for RORalpha. PMID: 14751813
    46. the orphan nuclear receptor RORalpha regulates bidirectionally Bmal1 (positively) and Per1 (negatively) transcriptions simultaneously PMID: 15135064
    47. ROR alpha has a role in the control of lipid homeostasis in skeletal muscle PMID: 15199055
    48. Rora, a circadian activator of brain-muscle-Arnt-like-protein 1 (Bmal1) transcription within the suprachiasmatic nucleus, is required for normal Bmal1 expression and consolidation of daily locomotor activity. PMID: 15312651
    49. The staggerer (sg) mutant mouse shows an early block in Purkinje cell (PC) development due to a truncation of the transcription factor retinoic acid-related orphan receptor alpha (ROR alpha). PMID: 15351503
    50. observations suggest that some of the actions of alpha-tocopherol (alpha-T) are mediated by the transcription factor retinoic acid-related orphan receptor alpha (ROR-alpha) PMID: 15753139

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  • 相关疾病：
    Defects in Rora are the cause of the staggerer (SG) mutant phenotype which is characterized by disturbance of Purkinje cell development and immune system functioning. This phenotype exhibits lower body weight, reduced adiposity, decreased plasma cholesterol, triglyceride and apolipoprotein CIII levels, and is resistant to diet-induced obesity. Also has abnormal circadian rhythms.
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Nuclear hormone receptor family, NR1 subfamily
  • 组织特异性：
    Expressed in cerebellum, heart, liver, lung, kidney, retina and brown and white adipose tissues. Expressed in the subset of mature Th17 cells.
  • 数据库链接：

    KEGG: mmu:19883

    STRING: 10090.ENSMUSP00000034766

    UniGene: Mm.378450