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  4. Recombinant Mouse Nuclear RNA export factor 1 (Nxf1)

Recombinant Mouse Nuclear RNA export factor 1 (Nxf1)

  • 货号：
    CSB-YP016218MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP016218MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP016218MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP016218MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP016218MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Nxf1
  • Uniprot No.：
  • 别名：
    Nxf1; TapNuclear RNA export factor 1; Tip-associated protein; Tip-associating protein; mRNA export factor TAP
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-618
  • 氨基酸序列
    ADEGKSYNE HDDRVSFPQR RKKGRGPFRW KCGEGNRRSG RGGSGVQSSR FEEDDGDVAM NDPQDGPRVR YNPYTNRPNR RGDGWHDRDR IHITVRRDRA PAERGGAGTS QDGTTKNWFK ITIPYGRKYD KTWLLSMIQS KCSVPFNPIE FHYENTRAHF FVEDATTASA LKGVNHKIQD RENRRISIII NASAPPYTVQ NELKPEQIEQ LKLIMSKRYD GNQQALDLKG LRSDPDLVAQ NIDVVLNRRS CMAATLRIIE ENIPELLSLN LSSNRLYKLD DMSSIVQKAP NLKTLNLSGN ELKTERELDK IKGLKLEELW LDRNPMCDNF GDQSSYISAI RERFPKLLRL DGHELPPPIS FDVEAPTMLP PCKGSYFGTE NLKSLVLRFL QQYYVIYDSG DRQGLLYAYH DGACCSLSIP YNPQNPVRKN LAEYVKDSRN VKKLKEPTQR FRLLKHTRLN VVAFLNELPK TQHDVNAFVV DISAQTSTLL CFSVNGVFKE VDGKSRDSLR AFTRTFIAVP ASNSGLCIVN DELFVRNASP EEIQRAFAMS APTPSSSPVP TLSPEQQEML QAFSTQSGMN LEWSQKCLQD NNWDYTRSAQ AFTLLKAKGE IPEVAFMK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm (TAP/NFX1 pathway). The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5 components of the TREX complex. ALYREF/THOC4-bound mRNA is thought to be transferred to the NXF1-NXT1 heterodimer for export. Also involved in nuclear export of m6A-containing mRNAs: interaction between SRSF3 and YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export.
  • 基因功能参考文献：
    1. Taken together, the results show for the first time that murine leukemia virus requires the Tap/NXF1-mediated export pathway for the expression of its spliced and unspliced RNAs and for full-legth RNA nuclear export. PMID: 24597485
    2. Disruption of NXF1 expression impaired cytoplasmic accumulations of both spliced and unspliced RNA transcripts of Murine leukemia virus. PMID: 24478440
    3. RNA transport element RTE evolved as a high affinity RBM15 ligand to provide a splicing-independent link to NXF1, thereby ensuring efficient nuclear export and expression of retrotransposon transcripts PMID: 17001072
    4. FMRP and Nxf2 associate with and destabilize Nxf1 mRNA in neuronal cells. PMID: 17548835
    5. Nxf1 suppresses a class of intracisternal A particle (IAP)-induced mutations, including Ap3d1(mh2J), a model for Hermansky-Pudlak syndrome, and Atcay(hes), a model for Cayman ataxia PMID: 19436707
    6. The RNA-binding motif protein 15B (RBM15B/OTT3) acts as cofactor of the nuclear export receptor NXF1. PMID: 19586903
    7. CAST allele of Nxf1 suppresses IAP-Idelta1 insertions in Ap3d1[mh2J], Atcay[hes], Mgrn1[md] and Usp14[axJ]. Does not suppress the Mitf[mi-bw] LINE nor any of six tested ETn insertion alleles. PMID: 19436707
    8. Allelic variation in Nxf1 suppresses RNA processing defects in IAP insertion alleles of Pitpn (vibrator) and Eya1 (branchio-oto-renal syndrome). PMID: 14517553

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  • 亚细胞定位：
    Nucleus, nucleoplasm. Nucleus speckle. Cytoplasm. Nucleus, nuclear pore complex. Nucleus envelope. Cytoplasm, Stress granule.
  • 蛋白家族：
    NXF family
  • 组织特异性：
    Expressed ubiquitously.
  • 数据库链接：

    KEGG: mmu:53319

    STRING: 10090.ENSMUSP00000010241

    UniGene: Mm.7271