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  4. Recombinant Mouse Nodal (Nodal)

Recombinant Mouse Nodal (Nodal)

  • 货号：
    CSB-YP015916MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP015916MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP015916MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP015916MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP015916MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Nodal; Nodal
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    245-354
  • 氨基酸序列
    HHLPDR SQLCRRVKFQ VDFNLIGWGS WIIYPKQYNA YRCEGECPNP VGEEFHPTNH AYIQSLLKRY QPHRVPSTCC APVKTKPLSM LYVDNGRVLL EHHKDMIVEE CGCL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Essential for mesoderm formation and axial patterning during embryonic development.
  • 基因功能参考文献：
    1. A cell that experiences the highest level of Nodal signaling begins to express Lefty1 and becomes an L1(epi) cell. Deletion of Lefty1 alone or together with Lefty2 increased the number of prospective DVE cells PMID: 29138408
    2. Overexpression of Nodal in mouse embryonic stem cells (ES) cells enhances mesoderm and endoderm specification, but inhibits neuroectoderm formation [Review]. PMID: 29206913
    3. Here the s report that zebrafish embryos without maternally provided vg1 fail to form endoderm and head and trunk mesoderm, and closely resemble nodal loss-of-function mutants. Although Nodal is processed and secreted without Vg1, it requires Vg1 for its endogenous activity. PMID: 29140251
    4. Inhibition of autocrine NODAL signaling did not alter kinetics of exit from the embryonic stem cells state but compromised both germline and somatic lineage specification. NODAL guides transition from naive to formative pluripotency. PMID: 28669603
    5. these results therefore highlight an unanticipated role for p53 family proteins in a regulatory network that integrates essential Wnt-Tcf and nodal-Smad inputs. PMID: 27889317
    6. In the presence of a Nodal heterozygous mutation mice lacking both Tgif1 and its paralog, Tgif2, survive to late gestation. PMID: 27924807
    7. PGAP6 plays a critical role in Nodal signaling modulation through CRIPTO shedding. PMID: 27881714
    8. results show that TET-mediated oxidation of 5-methylcytosine modulates Lefty-Nodal signalling by promoting demethylation in opposition to methylation by DNMT3A and DNMT3B; findings reveal a fundamental epigenetic mechanism featuring dynamic DNA methylation and demethylation crucial to regulation of key signalling pathways in early body plan formation PMID: 27760115
    9. Results indicate that microRNA miR-127 accelerates mesendoderm differentiation of embryonic stem cells through nodal signaling by targeting left-right determination factor 2 (Lefty2). PMID: 27072135
    10. Epigenetic histone deacetylation ensures neural fate commitment by restricting Nodal signalling in murine anterior epiblast ex vivo and mESC in vitro. PMID: 25904100
    11. The finding that Foxh1-Grg-mediated repression is not essential for Nodal expression during mouse embryogenesis suggests that other regulators compensate for the loss of repressive regulatory input that is mediated by Grg interactions. PMID: 25511461
    12. Nodal is epigenetically controlled through the epigenetic regulatory element in the mouse embryo and human cells. PMID: 25528267
    13. The Nodal morphogen gradient induces distinct gene expression patterns during zebrafish embryogenesis. PMID: 25869585
    14. HEB is a fundamental link between Nodal signalling, the derepression of a specific class of poised promoters during differentiation, and lineage specification in mouse embryonic stem cells PMID: 25775035
    15. conserved hotspot for the binding of pluripotency factors at the Nodal locus behaves as an enhancer, is activated ahead of other Nodal enhancers in the epiblast, and is essential to Nodal expression in embryonic stem cells PMID: 24960041
    16. Nodal signaling from the visceral endoderm is required to maintain Nodal gene expression in the epiblast and drive DVE/AVE migration. PMID: 25536399
    17. Studies indicate that the pair of Nodal and Lefty (Lefty1 and Lefty2) has a conserved role in left-right asymmetry. PMID: 24704359
    18. Meteorin is a novel regulator of Nodal transcription PMID: 24558432
    19. This implies that Gdf1 potentiates Nodal activity by stabilizing a low molecular weight fraction that is susceptible to neutralization by soluble Acvr2. PMID: 24798330
    20. The consequent prolonged Nodal activity in the node by the absence of Cerl2 affects local Nodal expression and prolongs its expression in the lateral plate mesoderm. PMID: 23544137
    21. The combined inhibition of Nodal and BMP signaling resulted in the accumulation of Nanog-negative cells, even in the presence of LIF, uncovering a shared role for BMP and Nodal signaling in maintaining Nanog expression and repression of differentiation. PMID: 23081664
    22. Snail knockdown abolished the Nodal-induced epithelial-mesenchymal transition in B16 cells PMID: 23348697
    23. findings show signaling by Nodal and its co-receptor Cripto is active during a crucial window of male germ cell development; germ cells of mutant mice with compromised Nodal signaling showed premature differentiation, reduced pluripotency marker expression and a reduced ability to form embryonic germ cell colonies PMID: 23034635
    24. A Nodal-to-TGFbeta cascade exerts biphasic control over cardiopoiesis. PMID: 22872153
    25. Study shows the cilia protein Arl13b is required for left right axis specification as its absence results in heterotaxia; the defect originates in the node where Cerl2 is not downregulated and asymmetric expression of Nodal is not maintained resulting in symmetric expression of both genes. PMID: 22554696
    26. Results show that Yin-Yang1 (YY1) is required for developmental processes, including epithelial to mesenchymal transition (EMT) and Nodal signaling. PMID: 22669107
    27. NODAL signaling, emanating from a maternal source in the uterus, is critical for proper placental development, adequate intrauterine growth, and term delivery of healthy offspring. PMID: 22378764
    28. Data suggest that expression of Nodal in pancreas is localized to islet beta-cells; up-regulation of Nodal expression increases apoptosis of beta cells and is associated with increased Alk7 expression. PMID: 22550067
    29. Our results allowed us to propose for the first time an autocrine role of Nodal during the development of germ cells and indicate that members of the TGB-beta family may reinforce the male fate and prevent meiosis in embryonic germ cells. PMID: 22396454
    30. Knockdown or pharmacological inhibition of the Nodal/Activin receptor Alk4/7 in cancer stem cells virtually abrogated their self-renewal capacity and in vivo tumorigenicity PMID: 22056140
    31. Distinct pathways for Nodal and Cripto in the differentiation of visceral endoderm and anterior visceral endoderm from extra-embryonic endoderm stem cells. PMID: 21862554
    32. Graded Nodal/Activin signaling mediates 3 distinct cell fate decisions in embryonic stem cells. PMID: 21731500
    33. Embryo transfer experiments indicate the embryo regulates Nodal expression in the uterus and directs its expression at the time of implantation, restricting NODAL to the sites between implantation crypts. PMID: 21270430
    34. asymmetry in pSmad2 distribution was found to be generated not by unidirectional transport of Nodal but rather as a result of left-right distribution of active Nodal in the node, which translated into asymmetry in lateral plate mesoderm PMID: 21419113
    35. In Nodal null embryos, membrane localisation of Dvl2 is reduced, while in mutants for the Nodal inhibitor Lefty1, Dvl2 is ectopically membrane localised, establishing a role for Nodal in modulating planar cell polarity signalling. PMID: 21364967
    36. Nodal signaling in embryonic stem cells was shown to recruit histone demethylase Jmjd3 to chromatin thereby counteracting Polycomb repression at target loci PMID: 20571128
    37. Nodal and its prime effector, FoxH1, are not essential to preimplantation Activin/Nodal signaling. PMID: 21047506
    38. Neural induction in embryonic stem cells requires retinoic acid at subnanomolar levels to suppress Nodal signaling and suggest that the mechanism by which Wnt signaling suppresses neural development is through facilitation of Nodal signaling. PMID: 20665854
    39. The patterning of forebrain and medial craniofacial elements requires a fine balance between BMP and Nodal signaling during primitive streak development. PMID: 20508035
    40. Data show that autocrine Nodal signaling in undifferentiated ES cells modulates the vital pluripotency pathway of BMP signaling. PMID: 20427282
    41. Cripto-1 activates nodal- and ALK4-dependent and -independent signaling pathways in mammary epithelial Cells. PMID: 11909953
    42. A conserved intronic enhancer (ASE), containing binding sites for the fork head transcription factor Foxh1, modulates dynamic patterns of Nodal expression during early mouse development. PMID: 12091315
    43. nodal signals through the activin type II receptors to specify the left-sidedness by means of a threshold mechanism PMID: 12112458
    44. Embryos lacking Nodal in the node fail to initiate molecular asymmetry in the left lateral plate mesoderm and exhibit multiple left-right patterning defects. PMID: 12231623
    45. Cerberus-like and Lefty-1 function redundantly to modulate Nodal signaling during gastrulation PMID: 12431380
    46. Nodal signalling during gastrulation is regulated by extraembryonic proteases Spc1 and Spc4 PMID: 12447384
    47. loss of Drap1 leads to severe gastrulation defects that are consistent with increased expression of Nodal and can be partially suppressed by Nodal heterozygosity PMID: 12471260
    48. Nodal produced in the node is required for expression of Nodal and other left side-specific genes in the lateral plate mesoderm PMID: 12654299
    49. There are Notch-responsive elements in the Nodal promoter, and a direct relationship between Notch activity and Nodal expression. PMID: 12730123
    50. Notch pathway acts upstream of Nodal expression during left-right asymmetry determination in mice. PMID: 12730124

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    TGF-beta family
  • 数据库链接：

    KEGG: mmu:18119

    STRING: 10090.ENSMUSP00000039653

    UniGene: Mm.57195