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  4. Recombinant Mouse Nicotinamide N-methyltransferase (Nnmt)

Recombinant Mouse Nicotinamide N-methyltransferase (Nnmt)

  • 货号：
    CSB-YP015906MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP015906MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP015906MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP015906MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP015906MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Nnmt
  • Uniprot No.：
  • 别名：
    NnmtNicotinamide N-methyltransferase; EC 2.1.1.1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-264
  • 氨基酸序列
    MESGFTSKDT YLSHFNPRDY LEKYYSFGSR HCAENEILRH LLKNLFKIFC LGAVKGELLI DIGSGPTIYQ LLSACESFTE IIVSDYTDQN LWELQKWLKK EPGAFDWSPV VTYVCDLEGN RMKGPEKEEK LRRAIKQVLK CDVTQSQPLG GVSLPPADCL LSTLCLDAAC PDLPAYRTAL RNLGSLLKPG GFLVMVDALK SSYYMIGEQK FSSLPLGWET VRDAVEEAGY TIEQFEVISQ NYSSTTSNNE GLFSLVGRKP GRSE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the N-methylation of nicotinamide using the universal methyl donor S-adenosyl-L-methionine to form N1-methylnicotinamide and S-adenosyl-L-homocysteine, a predominant nicotinamide/vitamin B3 clearance pathway. Plays a central role in regulating cellular methylation potential, by consuming S-adenosyl-L-methionine and limiting its availability for other methyltransferases. Actively mediates genome-wide epigenetic and transcriptional changes through hypomethylation of repressive chromatin marks, such as H3K27me3. In a developmental context, contributes to low levels of the repressive histone marks that characterize pluripotent embryonic stem cell pre-implantation state. Acts as a metabolic regulator primarily on white adipose tissue energy expenditure as well as hepatic gluconeogenesis and cholesterol biosynthesis. In white adipocytes, regulates polyamine flux by consuming S-adenosyl-L-methionine which provides for propylamine group in polyamine biosynthesis, whereas by consuming nicotinamide controls NAD(+) levels through the salvage pathway. Via its product N1-methylnicotinamide regulates protein acetylation in hepatocytes, by repressing the ubiquitination and increasing the stability of SIRT1 deacetylase. Can also N-methylate other pyridines structurally related to nicotinamide and play a role in xenobiotic detoxification.
  • 基因功能参考文献：
    1. Crystal structures of monkey and mouse nicotinamide N-methyltransferase (NNMT) bound with end product, 1-methyl nicotinamide have been presented. PMID: 28720493
    2. NNMT is a novel regulator of histone methylation, polyamine flux and NAD(+)-dependent SIRT1 signalling, and is a unique and attractive target for treating obesity and type 2 diabetes PMID: 24717514
    3. Brain NNMT expression was not altered in hyperhomocysteinemia. PMID: 21169389
    4. Adipose tissue is a source of nicotinamide N-methyltransferase and homocysteine. PMID: 18996527
    5. the progression of vascular inflammation and atherosclerosis was associated with the upregulation of hepatic nicotinamide N-methyltransferase activity and subsequent increase in endogenous MNA plasma levels PMID: 19307695

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Class I-like SAM-binding methyltransferase superfamily, NNMT/PNMT/TEMT family
  • 组织特异性：
    Expressed in white adipose tissue and liver (at protein level).
  • 数据库链接：

    KEGG: mmu:18113

    STRING: 10090.ENSMUSP00000034808

    UniGene: Mm.458542