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  4. Recombinant Mouse Neuronal migration protein doublecortin (Dcx)

Recombinant Mouse Neuronal migration protein doublecortin (Dcx)

  • 货号：
    CSB-YP006576MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP006576MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP006576MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP006576MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP006576MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Dcx
  • Uniprot No.：
  • 别名：
    Dcx; DcnNeuronal migration protein doublecortin; Doublin; Lissencephalin-X; Lis-X
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-366
  • 氨基酸序列
    MELDFGHFDE RDKASRNMRG SRMNGLPSPT HSAHCSFYRT RTLQALSNEK KAKKVRFYRN GDRYFKGIVY AVSSDRFRSF DALLADLTRS LSDNINLPQG VRYIYTIDGS RKIGSMDELE EGESYVCSSD NFFKKVEYTK NVNPNWSVNV KTSANMKAPQ SLASSNSAQA RENKDFVRPK LVTIIRSGVK PRKAVRVLLN KKTAHSFEQV LTDITEAIKL ETGVVKKLYT LDGKQVTCLH DFFGDDDVFI ACGPEKFRYA QDDFSLDENE CRVMKGNPSA AAGPKASPTP QKTSAKSPGP MRRSKSPADS GNDQDANGTS SSQLSTPKSK QSPISTPTSP GSLRKHKVDL YLPLSLDDSD SLGDSM
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May participate along with PAFAH1B1/LIS-1 in a distinct overlapping signaling pathway that promotes neuronal migration.
  • 基因功能参考文献：
    1. s found that 14-3-3epsilon binds to the microtubule binding protein, doublecortin (Dcx) in a phosphorylation dependent manner and that this binding increases the Dcx protein levels in the cell by interrupting ubiquitin based degradation. PMID: 28173130
    2. MEIS2 associates with chromatin-bound PBX1, recruits PARP1/ARTD1, and initiates PARP1-mediated eviction of H1 from the chromatin fiber. PMID: 28739678
    3. The regulation axis from microRNA-128-3p to doublecortin is critical for hippocampus-related contextual learning not only in wild type, but also in mice infused with Abeta-42. PMID: 27222923
    4. Findings demonstrate the direct requirement of ADAM10 in cortical radial migration and reveal the underlying mechanism by linking ADAM10-initiated regulated intramembrane proteolysis of Notch to the regulation of microtubule cytoskeleton through transcriptional control of Dcx expression PMID: 28158408
    5. Dcx is a new marker for the Pax7(+)MyoD(-) subpopulation, which contributes to myofiber maturation during muscle regeneration. PMID: 25480916
    6. DCX-positive cells resembling immature and developing neurons were found in mice and sheep and human hypothalamus. PMID: 24288185
    7. we showed that miR-29a directly regulated its target protein Doublecortin (DCX) expression, which further modulated axon branching in primary culture. PMID: 24535747
    8. Doublecortin knockout mice show lamination defects of the hippocampal pyramidal cell layer largely restricted to the CA3 region. PMID: 24073232
    9. Dcx protein- and mRNA-levels reflected changes in neurogenesis PMID: 23690918
    10. DCX is dispensable for the development of new neurons in adult mice PMID: 23667508
    11. results indicate that immature DCX-expressing neurons are required for successful acquisition of spatial learning, as well as reversal learning, but are not necessary for the retrieval of stored long-term memories. PMID: 23575857
    12. In axons from mice mutant for Dcx, widespread differences are found in actin-associated proteins as compared with wild-type axons PMID: 23303949
    13. analysis of doublecortin expressing neurons in the adult mouse dentate gyrus PMID: 22957010
    14. The Doublecortin specifically enhances binding of the ADP-bound Kif1a motor domain to microtubules. Dcx is essential for the function of Kif1a, a kinesin-3 motor protein that traffics synaptic vesicles. PMID: 22857951
    15. Whereas DCX mRNA levels significantly increase during acute hypoxia, down-regulation of DCX transcripts is found in response to chronic hypoxia compared to controls, in relation to specifically vulnerable brain regions. PMID: 21824737
    16. Our findings define doublecortin-like as a novel marker for specific aspects of adult neurogenesis, which partly overlap with DCX PMID: 22605579
    17. Data suggest that doublecortin (DCX) cells in the arcuate nucleus (ARC) represent a cellular correlate of plasticity that is involved in controlling energy balance. PMID: 22492306
    18. Our data reveal a dual reciprocal role for DCX phosphorylation in the regulation of microtubule and actin dynamics that is indispensable for proper brain lamination. PMID: 22367209
    19. Doublecortin is a new regulatory target of miR-134 during cortical neuronal differentiation, demonstrating a novel role for miR-134 in the modulation of cortical development. PMID: 21228099
    20. These data show that the double layer of cells from Dcx knockout mice have simplified dendritic arbors and an increased excitability as do cells of the less disorganized CA1 region PMID: 22250815
    21. DCX is thus, per se, no marker of neurogenesis but might be associated more broadly with plasticity. PMID: 22022443
    22. Data show that intracellular engulfment protein ELMO1 was required for phagocytosis by DCX(+) cells. PMID: 21804544
    23. DCX-positive neuroblasts were significantly decreased in the mouse somatosensory cortex and hippocampal CA1 region from postnatal day 14. PMID: 21360195
    24. articular and endochondral chondrocytes originate from the same mesenchymal cells that express Dcx PMID: 21162077
    25. These results suggest the physiological importance of phosphorylation of doublecortin for its function. PMID: 20715151
    26. Aging produces a dramatic decrease in the expression of doublecortin along with epigenetic modifications in the hippocampus. PMID: 20340168
    27. the proximal 3'-end region of the mouse Dcx regulatory sequence is essential for Dcx gene expression during differentiation of neuronal precursors. PMID: 20509865
    28. Transgenic ablation of doublecortin-expressing cells suppresses adult neurogenesis and worsens stroke outcome in mice. PMID: 20385829
    29. Thus, we provide definitive evidence for a critical role for Dcx in neuronal migration and neurogenesis, as well as for the in vivo genetic interaction of the two genes most commonly involved in human neuronal migration defects. PMID: 20181597
    30. Targeted mutation of Dcx gene causes disrupted hippocampal lamination; behavioral tests show defects in context and cued conditioned fear tests, suggesting deficits in hippocampal learning accompany the abnormal cytoarchitecture PMID: 12196578
    31. The coiled-coil domain of neurabin II binds to a DCX region encompassing the C-terminal portion of the second DCX repeat and the N-terminal portion of the Ser/Pro-rich domain. PMID: 14550532
    32. These results thus suggest that in addition to vesicular trafficking, DCX may play a role in the regulation of cell adhesion via its interaction with DFFRX in migrating and differentiating neurons. PMID: 15607950
    33. Dcx acts as a molecular link between microtubule and actin cytoskeletal filaments that is regulated by phosphorylation and neurabin II. PMID: 15632197
    34. Doublecortin expression levels in adult brain reflect neurogenesis. PMID: 15654838
    35. Transient recruitment of DCX to different scaffold proteins, JIP-1/2, which will regulate its phosphorylation by JNK, and Neurabin II, which will regulate its dephosphorylation by PP1, plays an important role in normal neuronal migration. PMID: 16530423
    36. Microtubule-associated protein Dcx is enriched at the leading edge in the branching and nucleokinesis of migrating interneurons. PMID: 16571605
    37. Our data identifies a critical function for DCX in the movement of newly generated neurons in the adult brain. PMID: 16699506
    38. Dcx is necessary not only for embryonic radial migration but also migration of adult subventricular zone cells PMID: 16931042
    39. Doublecortin (DCX) is one of the three genes found from Affymetrix gene chip analysis related to glioma patient survival. PMID: 17178868
    40. DCX has a distinct role in cortical interneuron migration; Dcx distribution is different in mutant mice compared with wild-type controls. PMID: 17409252
    41. Study finds that spinophilin, a protein-phosphatase 1 targeting protein, is responsible for the dephosphorylation of the microtubule-associated protein doublecortin Ser 297 selectively at the "wrist" of growing axons, leading to activation PMID: 17482550
    42. We used the in utero electroporation system to isolate a genomic fragment of the doublecortin gene that can direct reporter expression faithful to doublecortin in young neurons of the cerebral cortex. PMID: 17671991
    43. Results demonstrate that DCX can be used as a marker to distinguish articular chondrocytes from other chondrocytes and to evaluate the quality of tissue engineered or regenerated cartilage in terms of their "articular" or "non-articular" nature. PMID: 17897623
    44. Together, these results confirm that the LCS contributes pyramidal neurons to ventral forebrain structures and reveals that DCX and LIS1 have important functions in this major migratory pathway in the developing forebrain. PMID: 18075262
    45. seizures in Dcx KO mice are likely to be due to abnormal synaptic transmission involving heterotopic cells in the hippocampus and may therefore provide a useful model to further study how lamination defects underlie the genesis of epileptiform activities PMID: 18575605
    46. Data show that some of the nestin-positive cells co-express DCX in the peri-infarct area after photothrombosis. PMID: 18616932
    47. Expression of Doublecortin (Dcx) protein in the developing cerebellum of mouse from postnatal 2nd day to postnatal 22nd day and in young adults by immunohistochemistry. PMID: 18637555
    48. Data show that the greatest number of DCX-immunopositive cells was found in C57BL/6, which differed significantly from ICR and BALB/c mice. PMID: 18649926
    49. Results demonstrate that the majority of dorsal root ganglion neurons express doublecortin, both in somata and in fibers. PMID: 18803236
    50. DCX-EGFP-positive cells in the nonischemic subventricular zone of the transgenic mouse expressed high neural progenitor marker genes, Sox2 and nestin, but not mature neuronal marker genes PMID: 18854839

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  • 亚细胞定位：
    Cytoplasm. Cell projection, neuron projection.
  • 组织特异性：
    In neonatal tissues, highly expressed in brain, but not expressed in heart, liver, kidney and spleen. In adult tissues, faintly expressed in brain but not expressed in muscle, heart, lung, liver, spleen, intestine, kidney, testis and placenta.
  • 数据库链接：

    KEGG: mmu:13193

    STRING: 10090.ENSMUSP00000033642

    UniGene: Mm.12871