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  4. Recombinant Mouse Neuromodulin (Gap43)

Recombinant Mouse Neuromodulin (Gap43)

  • 中文名称：
    小鼠Gap43重组蛋白
  • 货号：
    CSB-YP009231MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Gap43重组蛋白
  • 货号：
    CSB-EP009231MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Gap43重组蛋白
  • 货号：
    CSB-EP009231MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Gap43重组蛋白
  • 货号：
    CSB-BP009231MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Gap43重组蛋白
  • 货号：
    CSB-MP009231MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Gap43
  • Uniprot No.：
  • 别名：
    Gap43; Basp2; Neuromodulin; Axonal membrane protein GAP-43; Calmodulin-binding protein P-57; Growth-associated protein 43
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-227
  • 氨基酸序列
    MLCCMRRTKQ VEKNDEDQKI EQDGVKPEDK AHKAATKIQA SFRGHITRKK LKGEKKGDAP AAEAEAKEKD DAPVADGVEK KEGDGSATTD AAPATSPKAE EPSKAGDAPS EEKKGEGDAA PSEEKAGSAE TESAAKATTD NSPSSKAEDG PAKEEPKQAD VPAAVTDAAA TTPAAEDAAT KAAQPPTETA ESSQAEEEKD AVDEAKPKES ARQDEGKEDP EADQEHA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    This protein is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction.
  • 基因功能参考文献：
    1. hnRNP-Q1-mediated repression of Gap-43 mRNA translation provides an additional mechanism for regulating GAP-43 expression and function and may be critical for neuronal development. PMID: 26658614
    2. The GASP-43 is involves in the orientation of cell division by interacting with Galphai. PMID: 26380950
    3. Trimethyltin decreased synaptophysin but not GAP-43 expression in the mouse hippocampus. PMID: 24001577
    4. Antiretroviral drugs may recruit the HuD-GAP43 pathway, potentially contributing to a response to the antiretroviral neuronal toxicity. PMID: 24565699
    5. KSRP modulation of GAP-43 mRNA stability restricts axonal outgrowth in embryonic hippocampal neurons. PMID: 24244461
    6. these findings suggest that GAP-43 is dynamically involved in early postnatal and adult hippocampal neurogenesis and synaptic connectivity, possibly contributing to the GAP-43+/- behavioral phenotype. PMID: 24576816
    7. In this review, GAP-43 in vivo knockdown in olivary neurons leads to the atrophy of their climbing fibers and a reduction in the ability to sprout toward surrounding denervated Purkinje cells. PMID: 23441024
    8. GAP-43 is required to sustain synaptic stability and promote the initiation of axonal regrowth PMID: 23754371
    9. In vitro results indicated that GAP43 is indeed expressed in both myoblasts and differentiating myotubes, and its cellular localization changes dramatically during maturation. PMID: 23308181
    10. Initial emergence of hollows in the somatosensory cortex is no different between GAP43 heterozygous mice and nonheterozygous littermate controls; however, the emergence of sides and septa is delayed by 2 days. PMID: 22759196
    11. This study demonistrated that neuronal responses to netrin-1 have been directly demonstrated to require GAP-43. PMID: 23085079
    12. Dynamic palmitoylation links cytosol-membrane shuttling of acyl-protein thioesterase-1 and acyl-protein thioesterase-2 with that of proto-oncogene H-ras product and growth-associated protein-43 PMID: 23396970
    13. Nos2 deficiency promotes medulloblastoma development in Ptch1(+/-) mice through retention of proliferating GCPs in the external granular layer due to reduced Gap43 expression PMID: 22438824
    14. GAP-43 expression in the mucosal myenteric plexus was markedly reduced in the small intestines of Neodiplostomum seoulense-infected mice. PMID: 22451741
    15. These data suggest the efficient transcription of the distal promoter of Gap43 is an important mark for the transition of P19 cells from the progenitor stage into neuronal differentiation. PMID: 21938722
    16. GAP-43 is essential both to maintain climbing fibres structure in non-traumatic condition and to promote sprouting after partial lesion of the inferior olive. PMID: 21695168
    17. The Gap43 and Pten network highlights the covariance of gene expression and forms a molecular network associated with axonal outgrowth in the adult retina. PMID: 21655357
    18. Results suggest that GAP-43 might link the cell membrane and spindle pole and consequently participate in controlling cleavage orientation during cell division. PMID: 21345365
    19. all the effects of environmental stimulation are amplified if the afferent Purkinje axons are endowed with enhanced intrinsic growth capabilities, induced by overexpression of GAP-43 PMID: 21304956
    20. Mice lacking one allele for GAP43 displayed resistance to change, stress-induced behavioral withdrawal and anxiety. GAP43 deficiency leads to the development of a subset of autistic-like behaviors. PMID: 20707874
    21. This study suggested abnormal postsynaptic differentiation in GAP-43 HZ cortex during early barrel development, followed by adaptive compensation and partial phenotypic rescue. PMID: 19915093
    22. permanently dephosphorylated GAP-43 may disrupt normal axonal fasciculation which gives rise to the ectopic growth into distal stratum oriens PMID: 19437419
    23. first evidence that links increased GAP-43 expression with growth of central axons; findings raise questions about the properties of granule cells and the mossy fiber mechanisms that differentially regulate axonal remodeling in the adult hippocampus PMID: 19650124
    24. GAP-43 promotes axon growth by multiple synergistic mechanisms that potentiate the intrinsic motility of the elongating processes, while reducing their sensitivity to environmental inhibition. PMID: 19895561
    25. Palmitoylcarnitine modulates the interaction of protein kinase C delta with GAP-43. PMID: 12061781
    26. While GAP-43 is not necessary for LTP induction, its phosphorylation may regulate presynaptic properties, thereby affecting synaptic plasticity and the induction of LTP in the hippocampus. PMID: 12099903
    27. Accumulation of GAP-43 along precise axon segments disrupts the normal axon-glia interaction and enhances the retraction of oligodendrocytic processes to facilitate the outgrowth of neuritic sprouts. PMID: 15009129
    28. nNOS-positive cells were surrounded by GAP-43 labeled regions that appeared to be presynaptic boutons. PMID: 15148562
    29. After status epilepticus in mice, early loss of GAP-43 and strong CD44 induction in the IML correlated with hilar cell loss and subsequent MFS. PMID: 15191797
    30. GAP-43 expressed in multipotent precursors is required for appropriate cell fate commitment, and its absence affects astrocyte as well as neuronal differentiation. PMID: 15234344
    31. Older transgenic animals overexpressing human S100B show more GAP-43 staining than control animals, suggesting synaptic remodeling could take place. PMID: 15306236
    32. The present results provide strong evidence for a pivotal role of hippocampal GAP-43 in the bidirectional regulation of mnemonic processing. PMID: 15390153
    33. GAP-43 is necessary for the development and function of a variety of neuronal systems. PMID: 15541878
    34. GAP-43 and L1 coexpressed in Purkinje cells act synergistically to switch these CNS neurons into a regeneration-competent phenotype and serve as a key regulator of the regenerative ability of intrinsic CNS neurons in vivo. PMID: 16195382
    35. modulator I repressed GAP-43 gene expression from the proximal promoter in non-neuronal cells; also, a novel stimulative element is immediately downstream of modulator I PMID: 16794332
    36. role of GAP-43 protein levels in regulating memory function PMID: 17554085
    37. GAP-43 plays a functional role in regulating neurotransmitter release, as determined in knockuot mice. PMID: 17581849
    38. GAP-43 is required to link centrosome position to process outgrowth and regulate neuronal polarity. PMID: 18235238
    39. an unregulated expression of HuD disrupts mossy fiber physiology in adult mice in part by altering the expression and phosphorylation of GAP-43 and the amount of free calmodulin available at the synaptic terminal PMID: 18493953
    40. TNF-alpha acts as an inducer of axonal growth into degenerated discs, as evidenced by decreased GAP-43 immunoreactivity in mice receiving TNF-deficient nucleus pulposus injections. PMID: 18552668
    41. the phosphorylation state of a single site on GAP-43 differentially regulates performance of three different memory-associated tasks. PMID: 18727047
    42. Multiple effects of GAP-43 on cerebellar development suggest that it is a critical downstream transducer of signaling. mechanisms PMID: 18777197
    43. NFAT-3 has a role as a direct transcriptional repressor of GAP-43 expression PMID: 19443652
    44. Our data demonstrate that expression of GAP-43 in fibroblasts results in lengthening of the cell cycle compared to control fibroblasts. The mechanism by which GAP-43 mediated this effect involved increasing the time spent in the G(1) phase. PMID: 19589379

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  • 亚细胞定位：
    Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Cell projection, filopodium membrane; Peripheral membrane protein. Perikaryon. Cell projection, dendrite. Cell projection, axon. Cytoplasm.
  • 蛋白家族：
    Neuromodulin family
  • 组织特异性：
    Expressed in the hippocampus (at protein level). Expressed in the dorsal root ganglion and the spinal cord (at protein level).
  • 数据库链接：

    KEGG: mmu:14432

    STRING: 10090.ENSMUSP00000099881

    UniGene: Mm.1222