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  4. Recombinant Mouse N-glycosylase/DNA lyase (Ogg1)

Recombinant Mouse N-glycosylase/DNA lyase (Ogg1)

  • 货号：
    CSB-YP016313MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP016313MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP016313MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP016313MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP016313MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ogg1; N-glycosylase/DNA lyase [Includes: 8-oxoguanine DNA glycosylase; EC 3.2.2.-); DNA-(apurinic or apyrimidinic site) lyase; AP lyase; EC 4.2.99.18)]
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-345
  • 氨基酸序列
    MLFRSWLPSS MRHRTLSSSP ALWASIPCPR SELRLDLVLA SGQSFRWKEQ SPAHWSGVLA DQVWTLTQTE DQLYCTVYRG DDSQVSRPTL EELETLHKYF QLDVSLAQLY SHWASVDSHF QRVAQKFQGV RLLRQDPTEC LFSFICSSNN NIARITGMVE RLCQAFGPRL IQLDDVTYHG FPNLHALAGP EAETHLRKLG LGYRARYVRA SAKAILEEQG GPAWLQQLRV APYEEAHKAL CTLPGVGAKV ADCICLMALD KPQAVPVDVH VWQIAHRDYG WHPKTSQAKG PSPLANKELG NFFRNLWGPY AGWAQAVLFS ADLRQPSLSR EPPAKRKKGS KRPEG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion.
  • 基因功能参考文献：
    1. OGG1 affects PRMT5 binding on histone H4 and the formation of dimethylation of histone H4 arginine-3 via PRMT5. PMID: 29409673
    2. This study demonstrated that the heterozygous Ogg1+/- mice exhibited poorer learning performance in the Barnes maze and Restoration by X-ray Irradiation. PMID: 29101721
    3. These observations imply that pre-excision step(s) during OGG1 initiated base excision repair evoked by reactive oxygen species facilitates NF-kappaB DNA occupancy and gene expression. PMID: 28266569
    4. we show for the first time that OGG1 plays a distinct role in regulation of blood hepatic glucose production. PMID: 29207315
    5. Data show that the total liver tumors was significantly higher in the N-diethylnitrosamine (DEN), N-methyl-N-nitrosourea (MNU), N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN), N-bis (2-hydroxypropyl) nitrosamine (DHPN) and 1,2-dimethylhydrazine dihydrochloride (DMH)-administered oxoguanine glycosylase 1 (Ogg1) knockout (-)(/)(-) males and females. PMID: 28820464
    6. 8-oxoguanine DNA glycosylase-1 (OGG1) is the essential protein involved in oxidative stress-induced DNA demethylation. PMID: 27251462
    7. OGG1 plays a role in an LSD1-dependent pathway of LPS-induced macrophage activation in mice. PMID: 28843610
    8. These findings demonstrate that OGG-1 negatively regulates inflammatory cytokine release by coordinating molecular interaction with the autophagic pathway in hyperoxia-induced lung injury. PMID: 28202616
    9. These results together, points to a new paradigm about the role of DNA damage and repair by OGG1 in aging and age-associated disease processes. PMID: 27343030
    10. results implicate hyperglycemia-induced O-GlcNAcylation of Ogg1 in increased mtDNA damage and, therefore, provide a new plausible biochemical mechanism for diabetic cardiomyopathy. PMID: 27816939
    11. DNA repair protein OGG1 bound to its substrate is coupled to DNA occupancy of NF-kappaB and functions in epigenetic regulation of gene expression. PMID: 27756845
    12. OGG1 plays a protective role in atherosclerosis by preventing excessive inflammasome activation. PMID: 27384322
    13. OGG1 acts as a STAT1 coactivator and has transcriptional activity in the presence of endotoxin PMID: 26496208
    14. Ogg1 and Mutyh regulate hippocampal gene expression related to cognition and behavior, suggesting a role for the glycosylases in regulating adaptive behavior. PMID: 26711335
    15. Data suggest that OGG1 plays a vital role in the protection of DNA bases from oxidative damage induced by radiofrequency electromagnetic radiation. PMID: 26401913
    16. Deletion of one or both alleles of ogg1 in mice does increase susceptibility to the toxic effects of aflatoxin B1. PMID: 25583175
    17. OGG1- DNA base excision repair plays a role in various biological processes that may benefit the host, but when in excess could be implicated in disease and/or aging processes. PMID: 25614460
    18. Oxidative damage and Ogg1 deficient background exacerbate repair deficiencies.. Overexpression of the arsenic metabolizing enzyme As3mt acts as adaptive mechanism. PMID: 26210637
    19. rise in the intracellular 8-oxoG base levels increases the proportion of GTP-bound Rac1 PMID: 23612479
    20. 8-Oxo-7,8-dihydroguanine repair is a lifetime process suggesting that, via Rho GTPase, OGG1 could be involved in the cytoskeletal changes and organ remodeling observed in various chronic diseases. PMID: 24681335
    21. Expression of OGG1 and APEX1 was decreased at 3h after last exposure to Aroclor 1254 and only the expression level of APEX1 was recovered at 24-h after, so inhibition of DNA repair can be a potential mode of action of Aroclor 1254 gonadal toxicity. PMID: 25113976
    22. These findings suggest an important role for AEC mtDNA integrity maintained by OGG1 in the pathogenesis of pulmonary fibrosis that may represent a novel therapeutic target. PMID: 24918270
    23. The data unveil a previously unidentified role of 8-Oxoguanine-DNA glycosylase-1 driven base excision repair pathway in the generation of endogenous signals for inflammation in the innate signaling pathway. PMID: 25267977
    24. Single cell gel electrophoresis (SCGE) and Pig-a mutation assay in vivo-tools for genotoxicity testing from a regulatory perspective: a study of benzo[a]pyrene in Ogg1(-/-) mice. PMID: 25308545
    25. Transient OGG1, APE1, PARP1 and Polbeta expression in an Alzheimer's disease mouse model. PMID: 24121118
    26. Ogg1 chaperoning of Aco-2 in preventing oxidant-mediated mtDNA damage and apoptosis may afford an innovative target for the molecular events underlying oxidant-induced toxicity. PMID: 24429287
    27. results show that nonproductive binding of OGG1 to 8-oxoG in promoter sequences could be an epigenetic mechanism to modulate gene expression for a prompt innate immune response. PMID: 24489103
    28. These observations suggest that Ogg1 overexpression may be an important mechanism to protect cardiac cells against oxidative stress damage. PMID: 24304833
    29. Oxidative stress and OGG1-dependent base excision repair mechanisms define the genetic integrity of mammalian spermatozoa. PMID: 23813448
    30. Data indicate that maternal folate depletion during pregnancy and high-fat feeding from weaning altered gene expression of Ogg1, Neil1, Mutyh and Xrcc1 in the brain of adult offspring. PMID: 23603834
    31. Data indicate that enzyme OGG1 plays a pivotal role in repairing mitochondrial DNA damage, and consequently, in mitochondrial reactive oxygen species production. PMID: 23748360
    32. OGG1 status is a critical determinant of risk for methylmercury toxicity, with increased susceptibility in Ogg1-deficient murine embryonic fibroblasts independent of cellular immortalization method. PMID: 23566953
    33. Decreased Ogg1 expression and thereby 8-oxoG repair in the airway epithelium conveyed a lower inflammatory response after pollen challenge of sensitized mice. PMID: 23127499
    34. 8-Oxoguanine DNA glycosylase deficiency increases susceptibility to obesity and metabolic dysfunction. PMID: 23284747
    35. OGG1 and MYH appear to be dispensable for antimutator function in mitochondria. PMID: 22209780
    36. OGG-1 deficiency negatively regulates allergen-induced airway inflammatory response PMID: 22100973
    37. OGG1 binding to PARP-1 plays a functional role in the repair of oxidative DNA damage. PMID: 22057269
    38. Cigarette smoke modulated the activity and distribution of the BER enzymes 8-oxoguanine-DNA-glycosylase (OGG1) and apyrimidinic/apurinic endonuclease (APE1) in several brain regions. PMID: 21778470
    39. Data suggest that Cockayne syndrome B protein protects CAG repeats from expansion by either active reduction of the tract length during parent-child transmission, or by antagonizing the action of OGG1, which tends to promote expansion in somatic cells. PMID: 21566259
    40. A frequently occurring mutagenic base lesion produced by ROS is 8-oxo deoxyguanine (8-oxo dG) and the major enzyme for repair of 8-oxo dG is 8-oxoguanine-DNA glycosylase 1 (OGG1). PMID: 21322094
    41. OGG1 has a pivotal role in repairing oxidative damage to nuclear DNA under ischemic conditions, thereby reducing brain damage and improving functional outcome PMID: 20736962
    42. These data indicate that the mitochondria are the major site for OGG1 repair activity under conditions of oxidative stress. PMID: 20042377
    43. The stoichiometry of base excision repair enzymes is one critical factor underlying the tissue selectivity of somatic CAG expansion. PMID: 19997493
    44. The expression of Cyp1a1, Cyp1a2, and OGG1genes was significantly up-regulated in mice given diets containing 375 ppm dicyclanil or more. PMID: 20118626
    45. study showed that 8-oxoguanine DNA glycosylase 1 is the only glycosylase that incises 8-oxoadenine, when base-paired with cytosine in mitochondria and nuclei, but a different enzyme incises 8-oxoadenine when base-paired with guanine in the nucleus PMID: 12644468
    46. In the striatum of ICR mice, Ogg1 was 34% and 31% lower than Balb/c and C57BL/J, respectively. Oxo8dG values in brains of C57BL/J mice were twice that of the values in brains of Balb/c and ICR mice. PMID: 12691525
    47. Findings show that hypermutation is unaffected in the absence of Ogg1 activity and indicate that 8-hydroxyguanine lesions most likely do not cause V gene mutations. PMID: 12759433
    48. OGG1 has a role in removing oxidized pyrimidines during repair of thymine glycols in mitochondrial DNA PMID: 12819227
    49. Deficiencies in Myh and Ogg1 predispose 65.7% of mice to tumors, predominantly lung and ovarian tumors, and lymphomas. PMID: 15126346
    50. OGG1 is a microtubule-associated protein itself or it utilizes yet to be identified motor proteins to ride on microtubules during interphase and mitosis and in response to oxidative DNA damage. PMID: 15474421

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  • 亚细胞定位：
    Nucleus, nucleoplasm. Nucleus speckle. Nucleus matrix.
  • 蛋白家族：
    Type-1 OGG1 family
  • 组织特异性：
    Highest expression in testis.
  • 数据库链接：

    KEGG: mmu:18294

    STRING: 10090.ENSMUSP00000032406

    UniGene: Mm.43612