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  4. Recombinant Mouse Mitotic spindle assembly checkpoint protein MAD1 (Mad1l1)

Recombinant Mouse Mitotic spindle assembly checkpoint protein MAD1 (Mad1l1)

  • 货号：
    CSB-YP895741MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP895741MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP895741MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP895741MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP895741MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Mad1l1; Mad1; Mitotic spindle assembly checkpoint protein MAD1; Mitotic arrest deficient 1-like protein 1; MAD1-like protein 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-717
  • 氨基酸序列
    MEDLGENTTV LSSLRSLNNF ISQRMEGTSG LDVSTSASGS LQKQYEYHMQ LEERAEQIRS KSYLIQVERE KMQMELSHKR ARVELERAAS TNARNYEREV DRNQELLARI RQLQECEATA EEKMREQLER HRLCKQNLDA VSQQLREQED SLASAREMIS SLKGRVSELQ LSAMDQKVQV KRLESEKQEL KEQLELQQRK WQEANQKIQE LQASQDERAE HEQKIKDLEQ KLCLQEQDAA VVKSMKSELM RMPRMERELK RLHEENTHLR EMKETNGLLT EELEGLQRKL SRQEKMQEAL VDLELEKEKL LAKLQSWENL DQTMGLNLRT PEDLSRFVVE LQQRELTLKE KNNSITSSAR GLEKVQQQLQ DEVRQANAQL LEERKKRETH EALARRLQKR NALLTKERDG MRAILGSYDS ELTQTEYSTQ LTQRLWEAED MVQKVHAHSS EMEAQLSQAL EELGVQKQRA DTLEMELKML KAQTSSAESS FSFCKEEVDA LRLKVEELEG ERSRLEQEKQ VLEMQMEKLT LQGDYNQSRT KVLHMSLNPI SMARQRQHED HDRLQEECER LRGLVHALER GGPIPADLEA ASSLPSSKEV AELRKQVESA ELKNQRLKEV FQTKIQEFRK VCYTLTGYQI DVTTESQYRL TSRYAEHQTD CLIFKATGPS GSKMQLLETE FSRSVPELIE LHLLQQDSIP AFLSALTIEL FSRQTSI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Forms a heterotetrameric complex with the closed conformation form of MAD2L1 (C-MAD2) at unattached kinetochores during prometaphase, recruits an open conformation of MAD2L1 (O-MAD2) and promotes the conversion of O-MAD2 to C-MAD2, which ensures mitotic checkpoint signaling.
  • 基因功能参考文献：
    1. MAD1 and Proto-Oncogene Proteins c-myc reciprocally regulate ribosomal DNA transcription, providing a mechanism for coordination of ribosome biogenesis and cell growth PMID: 15282543
    2. MAD1 is present in mouse oocytes at all stages during the first meiosis and that it participates in spindle checkpoint during meiosis PMID: 15342357
    3. Data show that the loss of Trrap leads to chromosome missegregation, mitotic exit failure and compromised mitotic checkpoints, which are caused by defective Trrap-mediated transcription of the mitotic checkpoint proteins Mad1 and Mad2. PMID: 15549134
    4. identified mouse Telomeric Repeat Binding Factor 1 (Trf1) as a protein that interacts directly with the spindle checkpoint protein Mad1 and the mitotic kinase Nek2 PMID: 15611654
    5. Together these data demonstrate that the MYC-antagonist MAD1 and cyclin-dependent kinase inhibitor p27(Kip1) cooperate to regulate the self-renewal and differentiation of HSCs in a context-dependent manner. PMID: 15654333
    6. the simultaneous knockdown of p18, p27 and MAD1 with a medium of only stem cell factor can induce long-term culture-initiating cell expansion despite the loss of engraftment ability PMID: 18685787

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  • 亚细胞定位：
    Nucleus. Chromosome, centromere, kinetochore. Nucleus envelope. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole.
  • 蛋白家族：
    MAD1 family
  • 数据库链接：

    KEGG: mmu:17120

    STRING: 10090.ENSMUSP00000031534

    UniGene: Mm.27250