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  4. Recombinant Mouse Mitogen-activated protein kinase 8 (Mapk8)

Recombinant Mouse Mitogen-activated protein kinase 8 (Mapk8)

  • 货号：
    CSB-YP013466MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP013466MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP013466MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP013466MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP013466MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Mapk8; Jnk1; Prkm8Mitogen-activated protein kinase 8; MAP kinase 8; MAPK 8; EC 2.7.11.24; Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-384
  • 氨基酸序列
    MSRSKRDNNF YSVEIGDSTF TVLKRYQNLK PIGSGAQGIV CAAYDAILER NVAIKKLSRP FQNQTHAKRA YRELVLMKCV NHKNIIGLLN VFTPQKSLEE FQDVYIVMEL MDANLCQVIQ MELDHERMSY LLYQMLCGIK HLHSAGIIHR DLKPSNIVVK SDCTLKILDF GLARTAGTSF MMTPYVVTRY YRAPEVILGM GYKENVDLWS VGCIMGEMVC HKILFPGRDY IDQWNKVIEQ LGTPCPEFMK KLQPTVRTYV ENRPKYAGYS FEKLFPDVLF PADSEHNKLK ASQARDLLSK MLVIDASKRI SVDEALQHPY INVWYDPSEA EAPPPKIPDK QLDEREHTIE EWKELIYKEV MDLEERTKNG VIRGQPSPLA QVQQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock. Phosphorylates the heat shock transcription factor HSF1, suppressing HSF1-induced transcriptional activity. Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteosomal degradation. Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1. In neurons, phosphorylates SYT4 which captures neuronal dense core vesicles at synapses. Phosphorylates EIF4ENIF1/4-ET in response to oxidative stress, promoting P-body assembly.
  • 基因功能参考文献：
    1. JNK acts as a key mediator of muscle remodeling during exercise via regulation of myostatin/SMAD signaling. PMID: 30072727
    2. These findings further validated the involvement of P. acnes in the pathology of intervertebral disc degeneration (IVDD) and provided evidence that P. acnes-induced apoptosis of NPCs via the TLR2/JNK pathway is likely responsible for the pathology of IVDD. PMID: 29323102
    3. High JNK expression is associated with cerebral ischaemia reperfusion injury. PMID: 30138595
    4. Noise exposure led to enhanced JNK phosphorylation and IRS1 serine phosphorylation as well as reduced Akt phosphorylation in skeletal muscles in response to exogenous insulin stimulation. PMID: 29433422
    5. Therefore, APP modulates Nav1.6 sodium channels through a Go-coupled JNK pathway, which is dependent on phosphorylation of APP at Thr668. PMID: 28008944
    6. this study establishes that JNK1 is a key mediator of osteoblast function in vivo and in vitro. PMID: 28561373
    7. Jnk1 deficiency inhibits the development of neural stem cells/precursors PMID: 27556303
    8. the effects of JNK1 deficiency in an experimental model of familial Alzheimer's disease, was investigated. PMID: 26558630
    9. Irradiation coupled with JNK inhibition in beta1 integrin -/- transgenic adenocarcinoma of prostate (TRAMP) leads to increased levels of nuclear focal adhesion kinase (FAK) in tumor cells. PMID: 27438371
    10. transgenic mice overexpressing sPLA2 -IIA keratinocytes showed enhanced activation of EGFR and JNK1/2 that led to c-Jun activation. PMID: 27299855
    11. p53 plays a novel protective role in APAP induced liver injury through inhibiting the activation of JNK, a key mediator in APAP-induced oxidative stress. PMID: 28196650
    12. BOC interacts with ABL and activates JNK thereby promoting neuronal differentiation and neurite outgrowth. PMID: 27871935
    13. Quantitative phosphoproteomic analysis identifies the critical role of JNK1 in neuroinflammation induced by Japanese encephalitis virus PMID: 27703031
    14. post-translational modification facilitates the mobilization of SIRT6 to DNA damage sites and is required for efficient recruitment of poly (ADP-ribose) polymerase 1 (PARP1) to DNA break sites and for efficient repair of double-strand break. PMID: 27568560
    15. The s have found that JNK signaling is required for proper vascular morphogenesis and the normal formation of collateral arteries in muscle. PMID: 27504807
    16. JNK1-mediated NLRP3 phosphorylation at S194 is a critical priming event and is essential for NLRP3 inflammasome activation. PMID: 28943315
    17. IL-6 likely up-regulates IRP1 and DMT1 expression and down-regulates FPN1 expression in BV2 microglial cells through JNK signaling pathways PMID: 28672025
    18. Study examined whether JNK is present at the presynaptic site and its activity after presynaptic NMDA receptors stimulation; found that JNK, via the JBD domain, acts as a physiological effector on T-SNARE proteins; data suggest that JNK-dependent phosphorylation of T-SNARE proteins may have an important functional role in synaptic plasticity. PMID: 28367336
    19. JNK signaling, which is inversely correlated with WNT4, plays an important role in perinatal germline cyst breakdown and primordial follicle formation by regulating E-cadherin junctions between oocytes in mouse ovaries. PMID: 27013242
    20. It was concluded that compounds targeting JNK1 activity in brain and adipose tissue, which do not accumulate in the skin, may be safer and most effective. PMID: 27230858
    21. JNK1 activation suppresses antifungal immunity in mice. JNK1-deficient mice had a significantly higher survival rate than wild-type control mice in response to Candida albicans infection, and the expression of JNK1 in hematopoietic innate immune cells was critical for this effect. PMID: 28112734
    22. activation of JNK in the endoplasmic reticulum stress response precedes activation of XBP1. PMID: 27122189
    23. JNK/FoxO1 is involved in the regulation of oxidative stress-induced cell apoptosis in MGCs PMID: 27936150
    24. apoE(-/-) mouse carrying a visceral fat graft from obese dnJNK donors were protected against enhanced systemic inflammation and atherogenesis. PMID: 27512097
    25. These data provide evidence for involvement of the TNF-alpha-JNK axis in extracellular matrix remodeling. PMID: 27875656
    26. Loss of Jnk1, but not Jnk2, in macrophages protects them from apoptosis, increasing cell survival, and this accelerates early atherosclerosis in LDL receptor knockout mice. PMID: 27102962
    27. Data indicate that in baicalein (BAI)-treated groups following AngII-infusion, p38-MAPK and JNK signaling were attenuated PMID: 27333787
    28. data suggest that c-Jun-N-terminal kinase 1 may be recruited by nicotine and is functionally necessary for the acute effects of nicotine on learning and memory. PMID: 27235579
    29. the results identify the JNK/S6K1 axis as a key molecular mechanism whereby a high fat/sucrose diet impairs insulin action in retina. PMID: 27965359
    30. MOR epigenetic regulation requires multiple coordinated signals converging at the MOR promoter, involving mitogen-activated protein kinase (MAPK) activation and mitogen- and stress-activated protein kinase 1. PMID: 28153853
    31. activation of astrocyte MMP2/JNK1/2 contributes to the pathogenesis of pain hypersensitivity in the complex regional pain syndrome model PMID: 27919822
    32. that JNK played roles in nerve regeneration at both early and late phases PMID: 26613205
    33. Results suggest that activation of JNK in Alzheimer's disease (AD) inhibits insulin signaling which could lead to a decreased expression of VGLUT1, therefore contributing to the glutamatergic deficit in AD PMID: 26836159
    34. The cJun NH2-terminal kinase contributes to the regulation of hepatic fibroblast growth factor 21 expression during fasting/feeding cycles. PMID: 26947074
    35. JNK kinase is critical for mitochondrial function during reperfusion after myocardial ischemia. PMID: 26746142
    36. Activated JNK promotes mitochondrial dysfunction and acute hepatotoxicity through robust phosphorylation of numerous mitochondrial proteins. PMID: 26491845
    37. Reactive oxygen species induce autophagy to counteract apoptosis in mesenchymal stem cells by activation of JNK. PMID: 26592514
    38. In hepatocytes, JNK1 and JNK2 appear to have combined effects in protecting from drug-induced liver injury. PMID: 26708719
    39. Diminished MTORC1-dependent JNK activation underlies the neurodevelopmental defects associated with lysosomal dysfunction. PMID: 26387958
    40. Role of JNK in the pathophysiology of hemorrhage/resususcitation-induced liver injury after chronic ethanol feeding PMID: 26367181
    41. Taken together, our findings demonstrate that salicortin inhibits NF-kappaB and NFATc1 activation, leading to attenuation of osteoclastogenesis and bone resorption. PMID: 26740180
    42. results suggest that JNK is a major negative regulator in mAChR-induced intestinal secretion. PMID: 25704031
    43. although JNK activation and RIP3 expression are induced by FS, neither contributes to the liver injury. PMID: 25423287
    44. Data suggest that JNK1 may not be critical for learning but when higher levels of hippocampus-dependent learning occur, JNK1 signaling is recruited and is necessary for stronger hippocampus-dependent memory formation. PMID: 25543025
    45. Quantitative proteomics reveals dynamic interaction of JNK with RNA transport granule proteins Sfpq and Nono during neuronal differentiation PMID: 25326457
    46. findings suggest that JNK is crucial for the development of Candida albicans cell wall extrac -induced vascular lesions in mice PMID: 25242023
    47. suppression of tumorigenesis by JNK requires ATF2. PMID: 25456131
    48. These experiments reveal both overlapping and nonredundant effects of JNK- and IKK-dependent signaling in AgRP neurons. PMID: 25456138
    49. JNK1 and JNK2 play primarily opposing roles in mucosal hyperplasia and neutrophil recruitment early in otitis media. PMID: 25311344
    50. Urotensin II inhibited the proliferation of cardiac side population cells by JNK/LRP6 signalling during pressure overload. PMID: 24447593

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Cell junction, synapse.
  • 蛋白家族：
    Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily
  • 组织特异性：
    Brain (at protein level).
  • 数据库链接：

    KEGG: mmu:26419

    STRING: 10090.ENSMUSP00000107576

    UniGene: Mm.21495