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  4. Recombinant Mouse Methylenetetrahydrofolate reductase (Mthfr)

Recombinant Mouse Methylenetetrahydrofolate reductase (Mthfr)

  • 货号：
    CSB-YP893882MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP893882MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP893882MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP893882MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP893882MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Mthfr
  • Uniprot No.：
  • 别名：
    Mthfr; Methylenetetrahydrofolate reductase; EC 1.5.1.20
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-654
  • 氨基酸序列
    MVNEARGSGS PNPRSEGSSS GSESSKDSSR CSTPSLDPER HERLREKMRR RMDSGDKWFS LEFFPPRTAE GAVNLISRFD RMAAGGPLFV DVTWHPAGDP GSDKETSSMM IASTAVNYCG LETILHMTCC QQRPEEITGH LHRAKQLGLK NIMALRGDPV GDHWEAEEGG FSYATDLVKH IRTEFADYFD ICVAGYPRGH PDAESFEDDL KHLKEKVSAG ADFIITQLFF EASTFFSFVK ACTEIGISCP ILPGIFPIQG YTSLRQLVKL SKLEVPQKIK DVIEPIKDND AAIRNYGIEL AVSLCRELLD SGLVPGLHFY TLNREVATME VLKQLGMWTE DPRRPLPWAL SAHPKRREED VRPIFWASRP KSYIYRTQDW DEFPNGRWGN SSSPAFGELK DYYLFYLKSK SPREELLKMW GEELTSEESV FEVFEHYLSG EPNRHGYRVT CLPWNDEPLA AETSLMKEEL LRVNRLGILT INSQPNINAK PSSDPVVGWG PSGGYVFQKA YLEFFTSRET VEALLQVLKT YELRVNYHIV DVKGENITNA PELQPNAVTW GIFPGREIIQ PTVVDPISFM FWKDEAFALW IEQWGKLYEE ESPSRMIIQY IHDNYFLVNL VDNEFPLDSC LWQVVEDTFE LLNRHPTERE TQAP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine.
  • 基因功能参考文献：
    1. findings indicate that the loss of folate as a methyl donor is a modifier of allergic airway disease, and that epigenetic and expression changes correlate with this modification. PMID: 29329322
    2. Ganglion cell loss and vasculopathy observed in Mthfr+/- and Cbs+/- mouse retinas may be milder than expected, not because of compensatory increases of enzymes in remethylation/transsulfuration pathways, but because downstream transsulfuration pathway products GSH, taurine, and H2S are maintained at robust levels. PMID: 28384716
    3. In summary, high folate intake during pregnancy leads to pseudo-MTHFR deficiency, disturbed choline/methyl metabolism, embryonic growth delay and memory impairment in offspring. These findings highlight the unintended negative consequences of supplemental folic acid. PMID: 28069796
    4. Study demonstrated that maternal MTHFR deficiency (i.e., in utero MTHFR deficiency) and early life exposure to vigabatrin separately and together alter the levels of proteins in the glutamatergic synapse PMID: 26235956
    5. Mildly hyperhomocysteinemic Mthfr+/- mice demonstrate reduced ganglion cell function, thinner NFL, and mild vasculopathy by 24 weeks. PMID: 25766590
    6. DNA methylation patterns in undifferentiated spermatogonia are relatively stable in culture over time under conditions of altered methionine and MTHFR levels. PMID: 24048573
    7. Data from Mthfr knockout mice (in homozygous/heterozygous matings) suggest that maternal genotype contributes to sensitivity to arsenic as embryotoxin (i.e., genetic predisposition to fetal resorption/congenital malformation in arsenic poisoning). PMID: 24384392
    8. our results support an interaction between mild neonatal stress, the MTHFR genotype and sex PMID: 23896051
    9. expressed in placenta at highest level during early development PMID: 22365888
    10. investigation of Mthfr regulation in an in vivo mouse model revealed temporal- and tissue-specific regulation that supports important roles for MTHFR in the developing embryo, and in postnatal brain and male reproductive tissues PMID: 21769670
    11. A possible mechanism for the epigenetic involvement of Mthfr deficiency is proposed in the gender-dependent regulation of proteins associated with plasticity of the excitatory synapse. PMID: 21490592
    12. These results showed that methylenetetrahydrofolate reductase deficiency impairs endothelial progenitor cell formation and increases endothelial progenitor cell senescence by endothelial nitric oxide synthase uncoupling and downregulation of SIRT1. PMID: 21169404
    13. Variable presentation of MTHFR deficiency in different genetic backgrounds; plasma homocysteine is not a predictor of severity. PMID: 20532821
    14. Newborn reflex development was slightly influenced by Mthfr +/- genotype and by the combination genotype and the neonatal vigabatrin (GVG) administration, in a sex-independent manner. Females presented attenuated anxiety due to Mthfr +/- genotype and GVG. PMID: 20813139
    15. multiple transcription start sites and alternative splicing PMID: 12370778
    16. reduces 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, the major carbon donor in the remethylation of homocysteine to methionine PMID: 14577615
    17. Mthfr(+/-) genotype sensitizes mice to diet-induced hyperhomocysteinemia and hyperhomocysteinemia alters tissue methylation capacity and impairs endothelial function in cerebral microvessels. PMID: 14630804
    18. These results suggest a possibility that the truncated pseudogene may buffer variations of the mouse Mthfr functional gene, and the mouse has evolved fewer variations of the gene than human PMID: 14637162
    19. important role for choline and betaine depletion in the pathogenesis of homocystinuria due to MTHFR deficiency PMID: 15217352
    20. MTHFR plays a role in cerebellar patterning, possibly through effects on proliferation or apoptosis. PMID: 15979267
    21. the elucidation of Mthfr regulation, demonstrates that two TATA-less, GC-rich promoters differentially drive transcription of Mthfr in a cell-specific manner, and provides a novel link of Mthfr to possible roles in the immune response and cell survival PMID: 16274753
    22. No interaction between Sp and Mthfr mutations, or between the Sp mutation and low dietary folate, in neural tube development, were observed. PMID: 16397891
    23. Review. A knockout mouse model for MTHFR deficiency displays hyperhomocysteinemia, homocystinuria, birth defects, and lipid metabolism alterations. PMID: 17349292
    24. Mild (methylenetetrahydrofolate reductase) MTHFR deficiency (heterozygotes) in mice is associated with increased risk for atherosclerotic disease PMID: 17689540
    25. Underexpression and overexpression of Mthfr/MTHFR increase MTX-induced myelosuppression but have distinct effects on plasma homocysteine and nephrotoxicity. PMID: 18551038
    26. VPA increases MTHFR expression and has lower teratogenic potential in MTHFR deficiency PMID: 18615588
    27. Maternal MTHFR and folate deficiencies resulted in increased developmental delays and smaller embryos. PMID: 19215022
    28. Hyperhomocysteinemia is associated with hypertriglyceridemia and that MTHFR deficiency may exacerbate lipid accumulation in ApoE deficiency. PMID: 19560954
    29. Mthfr deficiency protects against murine cytomegalovirus infection in vivo and in vitro PMID: 19609317

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  • 蛋白家族：
    Methylenetetrahydrofolate reductase family
  • 数据库链接：

    KEGG: mmu:17769

    STRING: 10090.ENSMUSP00000069774

    UniGene: Mm.89959