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  4. Recombinant Mouse Meiotic recombination protein SPO11 (Spo11)

Recombinant Mouse Meiotic recombination protein SPO11 (Spo11)

  • 货号：
    CSB-YP893873MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP893873MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP893873MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP893873MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP893873MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Spo11
  • Uniprot No.：
  • 别名：
    Spo11Meiotic recombination protein SPO11; EC 5.6.2.2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-396
  • 氨基酸序列
    MAFAPMGPEA SFFDALDRHR ASLLAMVKRG AGETPAGATR VASSSEVLTA IENIIQDIIK SLARNEVPAF TIDNRSSWEN IMFDDSVGLR MIPQCTTRKI RSDSPKSVKK FALILKVLSM IYKLIQSDTY ATKRDIYYTD SQLFGNQAAV DSAIDDISCM LKVPRRSLHV LSTSKGLIAG NLRYMEEDGT RVQCTCSATA TAVPTNIQGM QHLITDAKFL LIVEKDATFQ RLLDDNFCSR MSPCIMVTGK GVPDLNTRLL VKKLWDTFHI PVFTLVDADP YGIEIMCIYK YGSMSMSFEA HNLTIPTIRW LGLLPSDIQR LNIPKDSLIP LTKHDQMKLD SILKRPYITY QPLWKKELEM MADSKMKAEI QALTLLSSDY LSRVYLPNKL RFGGWI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of a topoisomerase 6 complex specifically required for meiotic recombination. Together with TOP6BL, mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination. The complex promotes relaxation of negative and positive supercoiled DNA and DNA decatenation through cleavage and ligation cycles. Essential for the phosphorylation of SMC3, HORMAD1 and HORMAD2.; In contrast to isoform 1, does not mediate DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination.
  • 基因功能参考文献：
    1. These data suggest that Tex19.1 and Ubr2 are required for mouse spermatocytes to accumulate sufficient Spo11-dependent recombination to ensure that the homology search is consistently successful, and reveal a hitherto unknown genetic pathway promoting meiotic recombination in mammals. PMID: 28708824
    2. If SPO11 expression is reduced and DNA double-strand breaks at zygonema are decreased (approximately 40 % below wild-type level), meiotic chromosome pairing is normal. PMID: 26440409
    3. suppression of genetic recombination in the pseudoautosomal region and at subtelomeres in mice with a hypomorphic Spo11 allele PMID: 23870400
    4. our results indicate that in addition to asynapsis, persistent SPO11-induced DSBs are important for the initiation of MSCI and MSUC, and that SPO11-independent DNA repair foci contribute to the MSUC response in oocytes. PMID: 23754961
    5. Mice with greater or fewer copies of the Spo11 gene--with correspondingly greater or fewer numbers of early recombination foci--exhibited relatively invariant crossover numbers. PMID: 22388890
    6. Data support a role for SPO11alpha in mid- to late prophase, presumably acting as a topoisomerase, that would be conserved in male and female meiocytes. PMID: 20647542
    7. required for meiotic chromosome synapsis PMID: 11106738
    8. chromosome synapsis defects and sexually dimorphic meiotic progression in Spo11 deficient mice PMID: 11106739
    9. Spo11 is not required for Ig hypermutation or class switch recombination PMID: 11807770
    10. In Spo11 -/- cells, SMC1 localization on meiotic chromosomes is mainly associated with homologous or non-homologous synapsed regions, whereas SMC3 localized throughout the chromosomes. Both localized along chromosome cores of Spo11 null spermatocytes. PMID: 12498344
    11. meiotic double-stranded breaks are processed by endonucleolytic cleavage that releases Spo11 attached to an oligonucleotide with a free 3'-OH PMID: 16107854
    12. The presence of these L-foci in Spo11(-/-) spermatocytes raises the interesting possibility of yet uncharacterized alterations in DNA or chromosome structure in Spo11(-/-) cells. PMID: 17456548
    13. Detection of the release product, SPO11-oligonucleotide complexes, from mouse testis lysates. PMID: 19799184

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    TOP6A family
  • 组织特异性：
    High levels are found only in the testis where expression is restricted primarily to meiotic germ cells. Not expressed in spermatogonia. Highest levels are found in pachytene spermatocytes. Very low levels are found in thymus, brain and oocytes of embryon
  • 数据库链接：

    KEGG: mmu:26972

    STRING: 10090.ENSMUSP00000059056

    UniGene: Mm.23495