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  4. Recombinant Mouse Malcavernin (Ccm2)

Recombinant Mouse Malcavernin (Ccm2)

  • 货号：
    CSB-YP816991MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP816991MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP816991MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP816991MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP816991MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Ccm2
  • Uniprot No.：
  • 别名：
    Ccm2; Osm; Cerebral cavernous malformations protein 2 homolog; Malcavernin; Osmosensing scaffold for MEKK3
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-453
  • 氨基酸序列
    MEEEGKKGKK PGIVSPFKRV FLKGEKSRDK KAHEKVTERR PLHTVVLALP ERVEPDRLLS DYIEKEVKYL GQLTSIPGYL NPSSRTEILH FIDKAKRSHQ LPGHLTQEHD AVLSLSAYNV KLAWRDGEDI ILRVPIHDIA AVSYVRDDAA HLVVLKTAQD PGISPSQSLC AESSRGLSAG SLSESAVGPV EACCLVIMAT ESKVAAEELC SLLSQVFQIV YTESTIDFLD RAIFDGASTP THHLSLHSDD SSTKVDMKDS YDADASTFCF PDSGDVGGLP PLPFCMQTSP HSKTVSESEL STSATELLQD YMLTLRTKLS SQEIQQFAAL LHEYRNGASI HEFCISLRQL YGDSRKFLLL GLRPFIPEKD SQHFENFLET IGVKDGRGII TDSFGRHRRA LSTTSTSTIN GNRTTGSPDD RSAPSEGDEW DRMISDISSD IEALGCSMDQ DSA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the CCM signaling pathway which is a crucial regulator of heart and vessel formation and integrity. May act through the stabilization of endothelial cell junctions. May also function as a scaffold protein for MAP2K3-MAP3K3 signaling. Seems to play a major role in the modulation of MAP3K3-dependent p38 activation induced by hyperosmotic shock.
  • 基因功能参考文献：
    1. Loss of CCM2 is associated with Cerebral Cavernous Malformations. PMID: 27513872
    2. CCM2 expression and it's role during ovary and testis development PMID: 26386873
    3. CCM2:MEKK3-mediated regulation of Rho-ROCK signalling is required for maintenance of neurovascular integrity, a mechanism by which CCM2 loss leads to disease. PMID: 26235885
    4. Down-modulation of STK25, but not STK24, rescued medulloblastoma cells from NGF-induced TrkA-dependent cell death, suggesting that STK25 is part of the death-signaling pathway initiated by TrkA and CCM2. PMID: 22782892
    5. The inducible deletion of Ccm2 in adult mice recapitulates the cerebral cavernous malformations-like brain lesions in humans. PMID: 21596842
    6. Developmental timing of CCM2 loss influences cerebral cavernous malformations in mice. PMID: 21859843
    7. Rac1/osmosensing scaffold for MEKK3 contributes via phospholipase C-gamma1 to activation of the osmoprotective transcription factor NFAT5. PMID: 21712438
    8. CCM1 associates with CCM2, indicating that the genetic heterogeneity observed in familial cavernous malformation pathogenesis may reflect mutation of different molecular members of a coordinated signaling complex. PMID: 16037064
    9. Ccm2 is expressed in neurons and choroid plexus but not in vascular endothelium of small vessels in the brain. PMID: 16465592
    10. embryo responses to hyperosmotic environments include elevation of CCM2 PMID: 17214902
    11. 14 bp deletion in the exon of CCM2 was present in eleven families from the Iberian Peninsula indicates a high prevalence of this mutation. PMID: 17345049
    12. CCM2 regulates endothelial cytoskeletal architecture, cell-to-cell interactions and lumen formation. Heterozygosity at Ccm2, a genotype equivalent to that in human CCM, results in impaired endothelial barrier function PMID: 19151728
    13. endothelial CCM2 has a role in proper vascular development PMID: 19259391

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  • 亚细胞定位：
    Cytoplasm. Note=Treatment with sorbitol caused relocalization to ruffle-like structures.
  • 蛋白家族：
    CCM2 family
  • 组织特异性：
    Highly expressed in heart, lower expression in kidney, lung and liver (at protein level).
  • 数据库链接：

    KEGG: mmu:216527

    STRING: 10090.ENSMUSP00000000388

    UniGene: Mm.221271