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  4. Recombinant Mouse MAP kinase-interacting serine/threonine-protein kinase 1 (Mknk1)

Recombinant Mouse MAP kinase-interacting serine/threonine-protein kinase 1 (Mknk1)

  • 货号：
    CSB-YP014603MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP014603MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP014603MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP014603MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP014603MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Mknk1; Mnk1; MAP kinase-interacting serine/threonine-protein kinase 1; EC 2.7.11.1; MAP kinase signal-integrating kinase 1; MAPK signal-integrating kinase 1; Mnk1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-427
  • 氨基酸序列
    MVSSQKLEKP IEMGSSEPLP IVDSDKRRKK KRKTRATDSL PGKFEDVYQL TSELLGEGAY AKVQGAVNLQ SGKEYAVKII EKQAGHSRSR VFREVETLYQ CQGNRNILEL IEFFEDDTRF YLVFEKLQGG SILAHIQKRK HFNEREASRV VRDVATALDF LHTKGIAHRD LKPENILCES PEKVSPVKIC DFDLGSGVKL NNSCTPITTP ELTTPCGSAE YMAPEVVEVF RDEATFYDKR CDLWSLGVVL YIMLSGYPPF VGHCGADCGW DRGEVCRMCQ NKLFESIQEG KYEFPDKDWA HISNEAKDLI SKLLVRDAKQ RLSAAQVLQH PWVQGQAPER GLPTPQVLQR NSSTMDLTLF AAEAIALNRQ LSQHEENELA EEQEALAEGL CSMKLSPPSK SRLARRRALA QAGRSRDANP CLTPAGL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.
  • 基因功能参考文献：
    1. MNK1 participates in mediating high-fat diet-induced insulin resistance. Our findings reveal distinct roles for the MNKs in a novel area of disease biology PMID: 27087055
    2. data suggest a physiological role for MNK1a-Ser(353) phosphorylation in regulation of the MNK1a kinase, which correlates with increased eIF4E phosphorylation in vitro and in vivo. PMID: 27413184
    3. Mnk1 is a novel pancreatic acinar cell-specific stress response kinase that regulates digestive enzyme abundance and eIF4E phosphorylation. PMID: 25037190
    4. Long-term potentiation (LTP) consolidation in the dentate gyrus of live rodents requires sustained (hours) BDNF-TrkB signaling and from TrkB to MAP-kinase-interacting kinase. PMID: 25453757
    5. Data suggest that Mnk1 and Mnk2 regulate cell migration/wound healing, expression of vimentin, stability of vimentin protein, and binding of eIF4E (eukaryotic translation initiation factor 4E) and Cyfip1 (cytoplasmic FMR1 interacting protein 1). PMID: 25588502
    6. Mnk1/2 has a minimal role in T cell development and activation but may regulate non-T cell lineages to control Th1 and Th17 differentiation in vivo. PMID: 23269249
    7. oncogenic role for Mnk1/2 in tumor development PMID: 20679220
    8. Mnk1 phosphorylation by caspase-activated Pak2/gamma-PAK inhibits phosphorylation and interaction of eIF4G with Mnk PMID: 15234964
    9. Mnk2 and Mnk1 are essential for constitutive and inducible phosphorylation of eukaryotic initiation factor 4E but not for cell growth or development PMID: 15254222
    10. Mnk activation by oxidative stress leading to enhanced eIF4E phosphorylation may play a role in promoting stress-induced hyperproliferative diseases, such as smooth muscle cell proliferation and hypertrophy in cardiovascular disease. PMID: 15683719
    11. The roles of features in the catalytic domains and C termini in determining the regulatory properties and basal activities of Mnk1 AND Mnk2 are reported. PMID: 16162500
    12. Roles of mitogen-activated protein kinase signal-integrating kinases 1 and 2 in oxidant-mediated eIF4E phosphorylation. PMID: 17689282
    13. mTOR inhibition increases eIF4E phosphorylation through a PI3K-dependent and Mnk-mediated mechanism. PMID: 17724079
    14. MNK1 and MNK2 are kinases involved in anti-apoptotic signaling in response to serum withdrawal PMID: 17903173
    15. Pharmacological inhibition of Mnk kinases or siRNA-mediated knockdown of Mnk1 and Mnk2 results in partial reversal of the suppressive effects of IFNalpha on normal and leukemic hematopoietic progenitors. PMID: 19574459

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  • 蛋白家族：
    Protein kinase superfamily, CAMK Ser/Thr protein kinase family
  • 组织特异性：
    Ubiquitously expressed in all tissues examined, with high levels in skeletal muscle.
  • 数据库链接：

    KEGG: mmu:17346

    STRING: 10090.ENSMUSP00000019677

    UniGene: Mm.209327