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  4. Recombinant Mouse M-phase inducer phosphatase 1 (Cdc25a)

Recombinant Mouse M-phase inducer phosphatase 1 (Cdc25a)

  • 货号：
    CSB-YP004994MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP004994MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP004994MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP004994MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP004994MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Cdc25a; Cdc25m3; M-phase inducer phosphatase 1; EC 3.1.3.48; Dual specificity phosphatase Cdc25A
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-514
  • 氨基酸序列
    MELGPEPPHR RRLFFACSPT PAPQPTGKML FGASAAGGLS PVTNLTVTMD QLEGLGSDCE KMEVRNNSSL QRMGSSESTD SGFCLDSPGP LDSKENLEIS LTRINSLPQK LLGCSPALKR SHSDSLDHDT FHLIDQDENK ENEAFEFKKP IRPASRHIYE ESKDPFTHRQ NSAPARMLSS NESESGNFSP LFIPQSPVKA TLSDEDDGFI DLLDGENMKN DEETPSCMAS LWTAPLVMRR PANLADRCGL FDSPSPCGSS TRAVLKRADR SHEEPPRGTK RRKSVPSPVK AKADVPEPAQ LPSQSLSLMS SPKGTIENIL DSDPRDLIGD FSKGYLFNTV SGKHQDLKYI SPEIMASVLN GKFAGLIKEF VIIDCRYPYE YEGGHIKGAV NLHMEEEVED FLLKNPIVPT DGKRVIVVFH CEFSSERGPR MCRYVRERDR LGNEYPKLHY PELYVLKGGY KEFFLKCQSH CEPPSYRPMH HEDFKEDLKK FRTKSRTWAG EKSKREMYSR LKKL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro. Phosphorylation by PIM1 leads to an increase in phosphatase activity.
  • 基因功能参考文献：
    1. Our results identify Cdc25A as a potential target for neuroprotectant strategy for the treatment of delayed ischemic neuronal death. PMID: 28607169
    2. CDC25A-deficient embryonic stem cells resist high doses of ATR inhibitors, which we show is due to their failure to prematurely enter mitosis in response to the drugs. PMID: 27067599
    3. These results showed that IGF1 regulated the expression of BOULE and CDC25A mRNAs via ERK1/2 signaling and in T-independent pathway during spermatogenesis in the adult mouse testes. PMID: 25726377
    4. accelerated cholangiocyte cystogenesis is likely due to overexpression of Cdc25A PMID: 24211536
    5. Cdc25A activity is required for the metaphase II arrest in mouse oocytes. PMID: 23345398
    6. deletion of Cdc25a increased apoptosis and accelerated the elimination of DNA damage following UV PMID: 22764135
    7. In the DNA damage response, instead of inhibiting cyclin B-CDK1 through destruction of Cdc25A phosphatase, oocytes utilize an inhibitory phosphorylation of Cdc25B. PMID: 22578416
    8. Cdc25A inhibitors block cell-cycle progression and proliferation, reduce liver and kidney weights and cyst growth in animal models of polycystic kidney/liver disease. PMID: 22155366
    9. CDC25A and CDC25B but not CDC25C compensate for each other to maintain the proliferative capacity of intestinal epithelial stem and progenitor cells PMID: 21283624
    10. IL-7 drives Cdc25A-mediated T-cell proliferation, which prevents the nuclear translocation of Foxo1, leading to reduced expression of CD62L and the migration of T cells into circulation. PMID: 20831893
    11. Results reveal an unexpected role of Cdc25A down-regulation and the inhibitory phosphorylation of cdk2 T14 and Y15 in cell cycle quiescence during muscle differentiation and implicate miRNAs-322 and -503 in the process. PMID: 20462953
    12. Cdc25A degradation does not inhibit Cdk2 activity because a considerable proportion of Cdk2 molecules localize to the cytoplasm and centrosomes in mESCs, where they may be sheltered from regulation by nuclear Cdc25A. PMID: 20104581
    13. human papillomavirus type 16 E7 maintains elevated levels of the cdc25A tyrosine phosphatase during deregulation of cell cycle arrest PMID: 11752153
    14. Identification of epidermal growth factor receptor as a target of Cdc25A protein phosphatase PMID: 11912208
    15. Studies of a transgenic mouse mammary tumor model identify Cdc25A as a candidate for human tumor latency modifier Apmt2. PMID: 12045150
    16. A naturally occurring point substitution in Cdc25A, and not Fv2/Stk, is associated with altered cell-cycle status of early erythroid progenitor cells. PMID: 12411323
    17. TGF-beta stimulates p160ROCK translocation to the nucleus and inhibitory phosphorylation of the cyclin-dependent kinase-activating phosphatase, Cdc25A. PMID: 14657354
    18. In lymphocyte cell lines dependent on interleukin-3 or interleukin-7, or primary lymphocytes dependent on interleukin 7, the phosphatase Cdc25A is the critical mediator of proliferation. PMID: 15928203
    19. Chk1/Cdc25A activity participates in activation of a cell cycle pathway-mediated death signal in neurons. PMID: 16928871
    20. Cdc25A plays a rate-limiting role in transformation and tumor initiation mediated by ras activation PMID: 17638870
    21. CDC25A is not only a major regulator of both G(1)/S and G(2)/M transition during unperturbed cell cycle progression, but also a critical checkpoint mediator.[review] PMID: 18073536
    22. restricting CDC25A can limit tumorigenesis induced by the HER2/neu-RAS oncogenic pathway without compromising normal cell division or viability [review] PMID: 18316586
    23. These data demonstrate that CDC25A behaves differently during female meiosis than during mitosis, and moreover, that CDC25A has a function in resumption of meiosis, spindle formation and the Meiosis I-Meiosis II transition. PMID: 18367163
    24. Cdc25A promotes G2/M transition in mouse oocytes. PMID: 18418039
    25. Results indicate that Cdc25A S123 phosphorylation is crucial for coupling centrosome duplication to DNA replication cycles after DNA damage and therefore is likely to play a role in the regulation of tumorigenesis. PMID: 18936171
    26. CDC25A is the only family member to provide an essential function during early embryonic development, and other family members compensate for its loss in adult mice. PMID: 19273838
    27. The expression of the dual specificity phosphatase CDC25A, is deregulated in Ba/F3 cells expressing the oncogenic protein NPM/ALK and in human cell lines derived from NPM/ALK-positive anaplastic large cell lymphomas. PMID: 19305144

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  • 蛋白家族：
    MPI phosphatase family
  • 组织特异性：
    Ubiquitously expressed in most developing tissue. High levels in the testis and lower levels in the ovary, particularly in germ cells. Lower levels also in kidney, liver, heart and muscle.
  • 数据库链接：

    KEGG: mmu:12530

    STRING: 10090.ENSMUSP00000091882

    UniGene: Mm.307103