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  4. Recombinant Mouse Lysine-specific demethylase 8 (Jmjd5)

Recombinant Mouse Lysine-specific demethylase 8 (Jmjd5)

  • 中文名称：
    小鼠Kdm8重组蛋白
  • 货号：
    CSB-YP887465MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Kdm8重组蛋白
  • 货号：
    CSB-EP887465MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Kdm8重组蛋白
  • 货号：
    CSB-EP887465MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Kdm8重组蛋白
  • 货号：
    CSB-BP887465MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Kdm8重组蛋白
  • 货号：
    CSB-MP887465MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Kdm8
  • Uniprot No.：
  • 别名：
    Kdm8; Jmjd5Bifunctional peptidase and arginyl-hydroxylase JMJD5; EC 1.14.11.-; EC 3.4.-.-; JmjC domain-containing protein 5; Jumonji C domain-containing protein 5; L-arginine; 3R)-hydroxylase KDM8; Lysine-specific demethylase 8
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-414
  • 氨基酸序列
    MSEDTTEPLV GSSTLWKELR TLLPDKEEEL KLDLGEKVDR SVAALLRQAV GLFYAGHWQG CLQASEAVLD YSWEKLNTGP WRDVDKEWRR VYSFGCLLKA LCLCQAPQKA TTVVEALRVC DMGLLMGAAI LEDILLKVVA VLQTHQLPGK QPARGPHQDQ PATKKAKCDA SPAPDVMLER MVPRLRCPPL QYFKQHFLVP GRPVILEGVA DHWPCMKKWS LQYIQEIAGC RTVPVEVGSR YTDEDWSQTL MTVDEFIQKF ILSEAKDVGY LAQHQLFDQI PELKRDISIP DYCCLGNGEE EEITINAWFG PQGTISPLHQ DPQQNFLVQV LGRKYIRLYS PQESEAVYPH ETHILHNTSQ VDVENPDLEK FPKFTEAPFL SCILSPGDTL FIPAKYWHYV RSLDLSFSVS FWWS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Bifunctional enzyme that acts both as an endopeptidase and 2-oxoglutarate-dependent monoxygenase. Endopeptidase that cleaves histones N-terminal tails at the carboxyl side of methylated arginine or lysine residues, to generate 'tailless nucleosomes', which may trigger transcription elongation. Preferentially recognizes and cleaves monomethylated and dimethylated arginine residues of histones H2, H3 and H4. After initial cleavage, continues to digest histones tails via its aminopeptidase activity. Upon DNA damage, cleaves the N-terminal tail of histone H3 at monomethylated lysine residues, preferably at monomethylated 'Lys-9' (H3K9me1). The histone variant H3F3A is the major target for cleavage. Additionnally, acts as Fe(2+) and 2-oxoglutarate-dependent monoxygenase, catalyzing (R)-stereospecific hydroxylation at C-3 of 'Arg-137' of RPS6 and 'Arg-141' of RCCD1, but the biological significance of this activity remains to be established. Regulates mitosis through different mechanisms: Plays a role in transcriptional repression of satellite repeats, possibly by regulating H3K36 methylation levels in centromeric regions together with RCCD1. Possibly together with RCCD1, is involved in proper mitotic spindle organization and chromosome segregation. Negatively regulates cell cycle repressor CDKN1A/p21, which controls G1/S phase transition. Required for G2/M phase cell cycle progression. Regulates expression of CCNA1/cyclin-A1, leading to cancer cell proliferation. Also, plays a role in regulating alpha-tubulin acetylation and cytoskeletal microtubule stability involved in epithelial to mesenchymal transition. Regulates the circadian gene expression in the liver. Represses the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer in a catalytically-independent manner. Negatively regulates the protein stability and function of CRY1; required for AMPK-FBXL3-induced CRY1 degradation.
  • 基因功能参考文献：
    1. The protease activities of JMJD5 and JMJD7 represent a mechanism for removal of histone tails bearing methylated arginine residues and define a potential mechanism of transcription regulation. PMID: 28847961
    2. Jmjd5 is involved in the transcriptional regulation of a subset of p53-regulated genes, possibly through the control of p53 recruitment at the gene loci. PMID: 26334721
    3. Data suggest that Jmjd5 partially accumulates on mitotic spindles during mitosis; depletion of Jmjd5 results in significant mitotic arrest, spindle assembly defects, and sustained activation of spindle assembly checkpoint. PMID: 26710852
    4. Collectively, these data indicate that JMJD5 is essential during embryonal development and a repressor of p53 expression. The latter suggests that JMJD5 has oncogenic activity and accordingly JMJD5 is upregulated in leukemias and breast cancer. PMID: 22402282
    5. Results suggest that Jmjd5 physiologically moderates embryonic cell proliferation through the epigenetic control of Cdkn1a expression. PMID: 22241836

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  • 亚细胞定位：
    Nucleus. Chromosome.
  • 组织特异性：
    Expressed in a circadian manner in the liver.
  • 数据库链接：

    KEGG: mmu:77035

    STRING: 10090.ENSMUSP00000033010

    UniGene: Mm.307239