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  4. Recombinant Mouse Lymphoid enhancer-binding factor 1 (Lef1)

Recombinant Mouse Lymphoid enhancer-binding factor 1 (Lef1)

  • 货号：
    CSB-YP012856MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP012856MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP012856MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP012856MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP012856MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Lef1; Lef-1; Lymphoid enhancer-binding factor 1; LEF-1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-397
  • 氨基酸序列
    MPQLSGGGGG GDPELCATDE MIPFKDEGDP QKEKIFAEIS HPEEEGDLAD IKSSLVNESE IIPASNGHEV VRQAPSSQEP YHDKAREHPD EGKHPDGGLY NKGPSYSSYS GYIMMPNMNS DPYMSNGSLS PPIPRTSNKV PVVQPSHAVH PLTPLITYSD EHFSPGSHPS HIPSDVNSKQ GMSRHPPAPE IPTFYPLSPG GVGQITPPIG WQGQPVYPIT GGFRQPYPSS LSGDTSMSRF SHHMIPGPPG PHTTGIPHPA IVTPQVKQEH PHTDSDLMHV KPQHEQRKEQ EPKRPHIKKP LNAFMLYMKE MRANVVAECT LKESAAINQI LGRRWHALSR EEQAKYYELA RKERQLHMQL YPGWSARDNY GKKKKRKREK LQESTSGTGP RMTAAYI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor that binds DNA in a sequence-specific manner. Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. PIASG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Required for IL17A expressing gamma-delta T-cell maturation and development, via binding to regulator loci of BLK to modulate expression. May play a role in hair cell differentiation and follicle morphogenesis.
  • 基因功能参考文献：
    1. we identified CYGB as an important regulator of osteosarcoma (OS) extravasation and highlighted the importance of the LEF1-CYGB regulatory axis in OS metastasis to the lungs. PMID: 29927026
    2. Lef1 and Tcf1 showed oncogenic effect in colonic carcinogenesis. Cellular context of miRNAs might play important roles in carcinogenesis by altering the expression pattern of Lef/Tcfs members. PMID: 27433921
    3. Data (including data from studies using transgenic/knockout mice) suggest that expression of Lef1 in hypothalamus inhibits anxiety and regulates neurogenesis; expression of Lef1 in hypothalamus is required for neurogenesis. PMID: 28837622
    4. Uncovering a Pitx2-Sox2-Lef-1 transcriptional mechanism that regulates dental epithelial stem cells homeostasis and dental development. PMID: 27660324
    5. this study shows that the transcription factors Tcf1 and Lef1 suppress CD4+ T lineage genes in CD8+ T cells through intrinsic histone deacetylase activity PMID: 27111144
    6. leukemic stem cells are therefore more sensitive to loss of Tcf1 and Lef1 than Hematopoietic Stem Cells in their self-renewal capacity. PMID: 27044748
    7. Sp5/8 bind directly to GC boxes in Wnt target gene enhancers and to adjacent, or distally positioned, chromatin-bound T-cell factor (Tcf) 1/lymphoid enhancer factor (Lef) 1 to facilitate recruitment of beta-catenin to target gene enhancers. PMID: 26969725
    8. This paper demonstrates a cell-intrinsic requirement for transcription factors TCF1 and LEF1 for the development of all subsets of Natural Killer T cells. PMID: 26490636
    9. data reveal LEF1 as a central regulator of iNKT cell number and Th2-type effector differentiation. PMID: 25897173
    10. These findings provide evidence for the existance of a posititve feedback loop connecting survivin expression in tumor cells to PI3K/Akt enhanced beta-catenin-Tcf/Lef-dependent transcription followed by secretion of VEGF and angiogenesis. PMID: 25204429
    11. TDG, as a new coactivator, promotes beta-catenin/TCFs transactivation and functionally cooperates with CBP in canonical Wnt signaling. PMID: 24748645
    12. TCF-1 and LEF-1 adopted distinct genetic 'wiring' to promote the CD4(+) T cell fate and establish CD8(+) T cell identity. PMID: 24836425
    13. Postnatal isoform switch and protein localization of LEF1 and TCF7L2 transcription factors in cortical, thalamic, and mesencephalic regions of the adult mouse brain. PMID: 23152144
    14. Activation of beta-catenin/TCF signaling by lysophosphatidic acid may be involved in neurogenesis by controlling the survival of neural precursors. PMID: 24145616
    15. LEF-1 induces epithelial-mesenchymal transition in a b-catenin- independent manner. PMID: 24269234
    16. data demonstrate that Lef-1 is an important factor for HSC and progenitor function and that its stem cell regulatory role depends on its DNA binding ability PMID: 23942465
    17. results confined the agenesis of the third molar (am3) locus to an interval of 1 Mbp on chromosome 3, demonstrating that Lef1, one of the essential transcription factors for early tooth development, is the strongest candidate for am3 PMID: 23736965
    18. Association of beta-catenin with P-Smad3 but not LEF-1 dissociates in vitro profibrotic from anti-inflammatory effects of TGF-beta1. PMID: 23203799
    19. LEF-1 was aberrantly upregulated in premalignant Tcf7(-/-) early thymocytes. PMID: 23103132
    20. LEF-1 and T cell factor (TCF)-1 cooperatively regulate generation of memory precursors and protective memory CD8+ T cells. PMID: 22875805
    21. functional TCF/Lef1 signaling governs important aspects of epidermal differentiation and lipid metabolism, thereby regulating skin barrier function. PMID: 21938009
    22. Lgr4-deficient mice showed premature differentiation of ureteric bud with reduced expression of Wnt effector Lef1 and Gata3. PMID: 21523854
    23. These data demonstrate that aberrant signalling by TCF/Lef1, transcription factors crucial for hair follicles bulge stem cell activation and hair differentiation, results in development of ectopic sebaceous glands originating from bulge cells. PMID: 21694721
    24. a novel interaction between the VDR and LEF1 that is mediated by the DNA-binding domain of the VDR and that is required for normal canonical Wnt signaling in keratinocytes. PMID: 21471213
    25. Lef1DeltaN binds beta-catenin, stimulates Lef/Tcf reporter activity, and promotes terminal osteoblast differentiation. PMID: 21270130
    26. LEF-1 and beta-catenin bind Pitx2 promoter sequences in ocular neural crest, indicating a likely direct effect of canonical Wnt signaling on Pitx2 expression. PMID: 20960542
    27. Lef1 has a positive effect on the self-renewal of mouse embryonic stem cells PMID: 20659708
    28. beta-catenin, Lef1 & Tcf1 are needed for Bmp4 expression maintaining Shh expression in the dental ectoderm. The number of incisors from one placode can be varied through local changes of a mesenchymal signaling circuit involving these proteins. PMID: 20883686
    29. miR-26b regulates Pit-1 expression by inhibiting Lef-1 expression and may promote Pit-1 lineage differentiation during pituitary development. PMID: 20807761
    30. Data demonstrate that Sox17 can directly regulate Wnt/beta-catenin-dependent transcription of the Lef-1 promoter and reveal new context-dependent binding sites in the Lef-1 promoter that facilitate protein-protein interactions between Sox17 and TCF4. PMID: 20802155
    31. The results suggested that Wnt/beta-catenin signaling could function in enamelin gene expression by direct interaction through two conserved LEF1 responsive elements on the enamelin gene in ameloblast-like cells. PMID: 20599763
    32. Drastic down-regulation of Lef1 observed at both E16 and E18; RT-PCR revealed post-natal reduction in expression (Lef1, 1/37). Results suggest down-regulation of gene is important factor in normal odontogenesis in dental papillae. PMID: 20448247
    33. Data indicate Lef1 binds Pax3 & the complex binds to Lef1 promoter at Lef1 binding sites to enhance transcription of Lef1. PMID: 20006729
    34. In adult mouse brain, beta-catenin and LEF1 accumulate in the nuclei of neurons specifically in the thalamus. PMID: 20371816
    35. In the canonical Wnt signaling pathway, beta-catenin can partner with Lef-1/Tcf high mobility group (HMG) box transcription factors to control gene expression PMID: 11854293
    36. The LEF1/beta -catenin complex activates movo1 PMID: 11983900
    37. LEF-1 as key regulator of beta-catenin nuclear localization and stability PMID: 11986304
    38. Nr-CAM is the gene most extensively induced by LEF1 PMID: 12183361
    39. LEF-1 participates in the regulation of the recombination-activating gene-2 promoter together with c-Myb and Pax-5 in immature B cells. PMID: 12244173
    40. LEF1 repressed Runx2-induced activation of the mouse osteocalcin 2 promoter in several osteoblast lineage cell lines. PMID: 12551949
    41. An intricate molecular program links two extracellular signalling pathways to the formation of a nuclear transcription factor that acts on target genes to remodel cellular junctions and permit hair follicle formation PMID: 12646922
    42. Redundant functions of TCF-1 and LEF-1 during T and NK cell development, but unique role of TCF-1 for Ly49 NK cell receptor acquisition. PMID: 12731066
    43. interaction with Smad4 and beta-catenin assists in regulating graded expression of Msx2 PMID: 14551209
    44. Smad2,4/LEF1 complex replaces beta-catenin/LEF1 during activation of epithelial mesenchymal transformation in vivo by TGFbeta3. PMID: 14691138
    45. Wnt regulation of the Lef-1 promoter at the WRE may play an important role during airway submucosal glandular bud formation. PMID: 15194563
    46. cellular mechanism of LEF1 signaling in regulating tooth morphogenesis PMID: 15649466
    47. LEF-1 expression is regulated through PITX2, LEF-1 and beta-catenin direct physical interactions PMID: 15728254
    48. beta-catenin displaces Groucho/TLE from Tcf/Lef by binding to a previously unidentified second, low-affinity binding site on Lef-1 PMID: 15768032
    49. We propose that there are two distinct pathways for the nuclear import of lef-1: importin alpha/beta-mediated and beta-catenin-mediated one, which provides a novel paradigm for Wnt signaling pathway. PMID: 15936755
    50. LEF1, a downstream component of the Wnt signaling pathway, mediates breast cancer cell invasion, and may be regulated in part by estradiol PMID: 16142310

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  • 亚细胞定位：
    Nucleus. Note=Found in nuclear bodies upon PIASG binding.
  • 蛋白家族：
    TCF/LEF family
  • 组织特异性：
    Expressed in Vgamma1.1 and Vgamma2 gamma-delta T-cells, however not expressed in gamma-delta thymocytes fated for Il17a expression (at protein level). Expressed in alpha-beta T-cell lineages. Expressed in the thymus. Found in distinct epithelial cell comp
  • 数据库链接：

    KEGG: mmu:16842

    STRING: 10090.ENSMUSP00000029611

    UniGene: Mm.255219