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  4. Recombinant Mouse Leukosialin (Spn), partial

Recombinant Mouse Leukosialin (Spn), partial

  • 货号：
    CSB-YP022590MO1
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP022590MO1
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP022590MO1-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP022590MO1
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP022590MO1
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Spn; Leukosialin; B-cell differentiation antigen LP-3; Leukocyte sialoglycoprotein; Lymphocyte antigen 48; Ly-48; Sialophorin; CD antigen CD43
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. Positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN). Negatively regulates Th2 cell differentiation and predisposes the differentiation of T-cells towards a Th1 lineage commitment. Promotes the expression of IFN-gamma by T-cells during T-cell receptor (TCR) activation of naive cells and induces the expression of IFN-gamma by CD4(+) T-cells and to a lesser extent by CD8(+) T-cells. Plays a role in preparing T-cells for cytokine sensing and differentiation into effector cells by inducing the expression of cytokine receptors IFNGR and IL4R, promoting IFNGR and IL4R signaling and by mediating the clustering of IFNGR with TCR. Acts as a major E-selectin ligand responsible for Th17 cell rolling on activated vasculature and recruitment during inflammation. Mediates Th17 cells, but not Th1 cells, adhesion to E-selectin. Acts as a T-cell counter-receptor for SIGLEC1.; Protects cells from apoptotic signals, promoting cell survival.
  • 基因功能参考文献：
    1. glycosylation status of CD43 protein on leukemia cells is associated with sensitivity to CTL-mediated cytolysis PMID: 27011118
    2. findings demonstrate that CD43 is a major E-selectin ligand in Th17 cells that functions independent of P-selectin glycoprotein ligand-1 PMID: 26700769
    3. CD43 may be an important mediator of macrophage lipid homeostasis, thereby affecting macrophage foam cell formation and ultimately atherosclerotic plaque development. PMID: 25524774
    4. We have identified a robust physiologic role for CD43 in a relevant animal model and established an important in vivo function for CD43-dependent regulation of IFN-gamma production. PMID: 23585675
    5. Results show that while CD43 binding to ezrin-radixin-moesin proteins is crucial for S76 phosphorylation, CD43 movement and regulation of T-cell migration can occur through an ERM-independent, phosphorylation-dependent mechanism. PMID: 21289089
    6. study found that the M. tuberculosis Cpn60.2 molecular chaperone is the major mycobacterial surface ligand that interacts with CD43 to stabilize the interaction between M. tuberculosis and the macrophage PMID: 20633027
    7. Transient resistance to B16F10 melanoma growth and metastasis in CD43-/- mice. PMID: 11828253
    8. CD43 engagement and signaling are involved in the costimulatory and antiapoptotic effects of enzymatically inactive mutant trypanosomal trans-sialidase when it binds highly sialylated mucin CD43 on the T cell surface. PMID: 11994475
    9. Cd43 displays dynamic roles in T cell immunity: a positive regulatory role in costimulation and trafficking of T cells to the central nervous system, and a negative regulatory role in the down-modulation of an immune response. PMID: 12055210
    10. Delayed onset of experimental autoimmune encephalomyelitis in CD43-deficient immunized mice and in recipients of adoptively transferred CD43-/- cells is characterized by significant reduction of T cell trafficking to the brain and spinal cord. PMID: 14662853
    11. Exclusion of the CD43 intracellular region from the immunological synapse is required for CD43 regulation of interleukin-2 production. PMID: 15117976
    12. Two functionally distinct populations of small intestinal intraepithelial lymphocytes (IELs) are described that differ according to the expression of a CD43 glycoform. PMID: 15528368
    13. CD43 is involved in the binding, uptake, and subsequent growth of Mycobacterium tuberculosis in murine macrophages and in vivo PMID: 16034122
    14. That CD43 costimulation was responsible for elevated cytokine/chemokine activity was confirmed at the transcriptional level by real-time PCR for IFN-gamma and CCL5, and by ELISA for IFN-gamma. PMID: 16246302
    15. CD43, when modified by a specific set of glycosyltranferases, can function as an E-selectin ligand and therefore potentially mediate activated T cell migration PMID: 16339541
    16. These results characterize an important role for CD43 during the activation and expansion of autoreactive MOG-specific CD8+ T cells. PMID: 16890924
    17. Recruitment of neutrophils and activated T cells in in vivo models is not influenced by CD43 expression. PMID: 17056577
    18. CD43 functions as a signaling molecule, sensing extracellular cues and transducing intracellular signals that modulate T-cell function PMID: 17638845
    19. results demonstrate that CD43 regulates Th cell differentiation in vitro and in vivo PMID: 18490738
    20. The s propose that CD43 is involved in the inflammatory response to M. tuberculosis and, through the induction of pro-inflammatory mediators, can regulate apoptosis to control intracellular growth of the bacterium. PMID: 18637079
    21. The loss of both PSGL-1 and CD43 led to a further increase in T cell adhesion and proliferation PMID: 19890058

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  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein. Cell projection, microvillus. Cell projection, uropodium.; [CD43 cytoplasmic tail]: Nucleus. Nucleus, PML body.
  • 组织特异性：
    Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.
  • 数据库链接：

    KEGG: mmu:20737

    STRING: 10090.ENSMUSP00000049534

    UniGene: Mm.283714