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  4. Recombinant Mouse Junction plakoglobin (Jup)

Recombinant Mouse Junction plakoglobin (Jup)

  • 货号：
    CSB-YP011975MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP011975MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP011975MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP011975MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP011975MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Jup
  • Uniprot No.：
  • 别名：
    Jup; Junction plakoglobin; Desmoplakin III; Desmoplakin-3
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-745
  • 氨基酸序列
    MEVMNLIEQP IKVTEWQQTY TYDSGIHSGV NTCVPSVSSK GIMDEDDACG RQYTLKKTTT YTQGVPQNQG DLEYQMSTTA RAKRVREAMC PGVSGEDSSL LLATQVEGQT TNLQRLAEPS QLLKSAIVHL INYQDDAELA TRALPELTKL LNDEDPVVVT KAAMIVNQLS KKEASRRALM GSPQLVAAVV RTMQNTSDLD TARCTTSILH NLSHHREGLL AIFKSGGIPA LVRMLSSPVE SVLFYAITTL HNLLLYQEGA KMAVRLADGL QKMVPLLNKN NPKFLAITTD CLQLLAYGNQ ESKLIILANG GPQGLVQIMR NYSYEKLLWT TSRVLKVLSV CPSNKPAIVE AGGMQALGKH LTSNSPRLVQ NCLWTLRNLS DVATKQEGLE SVLKILVNQL SVDDVNVLTC ATGTLSNLTC NNSKNKTLVT QNSGVEALIH AILRAGDKDD ITEPAVCALR HLTSRHPEAE MAQNSVRLNY GIPAIVKLLN QPNQWPLVKA TIGLIRNLAL CPANHAPLQE AAVIPRLVQL LVKAHQDAQR HVAAGTQQPY TDGVRMEEIV EGCTGALHIL ARDPMNRMEI FRLNTIPLFV QLLYSSVENI QRVAAGVLCE LAQDKEAADA IDAEGASAPL MELLHSRNEG TATYAAAVLF RISEDKNPDY RKRVSVELTN SLFKHDPAAW EAAQSMIPIN EPYADDMDAT YRPMYSSDVP LDPLDMHMDL DGDYPMDTYS DGLRPPYPTA DHMLA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
  • 基因功能参考文献：
    1. Reduced plakoglobin staining in intercalated discs of heart tissue from human arrhythmogenic right ventricular cardiomyopathy (ARVC) patients and in a murine ARVC model is caused by alterations in epitope accessibility and not by protein relocalization PMID: 26676851
    2. Mice with hepatic loss of the desmosomal protein gamma-Catenin are prone to cholestatic injury and chemical carcinogenesis. PMID: 26485505
    3. EMMPRIN ensures proper actomyosin-driven maturation of competent endothelial junctions by forming a molecular complex with gamma-catenin (also known as junction plakoglobin) and Nm23 (also known as NME1), a nucleoside diphosphate kinase PMID: 24994937
    4. In 2 knockin mouse models, endogenous Jup was engineered to express the Naxos-associated form of plakoglobin. Insufficiency of the truncated Naxos plakoglobin, rather than gain of function, accounted for cardiac disease manifestations. PMID: 25705887
    5. plakoglobin (Pg) in conjunction with lymphoid enhancer-binding factor 1 (Lef-1) differentially regulates the proximal promoters of Dsc2 and Dsc3 PMID: 23652796
    6. our study reveals a function for gamma-catenin in the regulation of mESC differentiation and has implications for human cancers in which gamma-catenin is mutated and/or aberrantly expressed. PMID: 23724138
    7. The data suggest novel function(s) for plakoglobin (PG) beyond the heart and define a critical threshold of PG expression that is necessary for postnatal survival. PMID: 22522917
    8. decreased plakoglobin expression increases the invasive behavior of breast cancer cells; this is the first demonstration of a functional role for plakoglobin/gamma-catenin in the metastatic process PMID: 22632416
    9. The cadherin-catenin signaling pathway was inactivated in spinal motor neurons to assess the significance of motor neuron position in motor circuit assembly; genetic inactivation of both beta- and gamma-catenin or N-cadherin, disrupts motor neuron positioning. PMID: 22036570
    10. Lack of plakoglobin in epidermis leads to keratoderma. PMID: 22315228
    11. Loss of Ncad-binding proteins beta-catenin and plakoglobin in the heart leads to gap junction remodeling and abrupt onset of spontaneous lethal ventricular arrhythmia in double knockout mice. PMID: 22252313
    12. While gamma-catenin gene expression remained unaltered, an increase in serine- and threonine-phosphorylated, but not tyrosine-phosphorylated gamma-catenin was observed in beta-catenin knockout livers. PMID: 21703193
    13. Ventricular arrhythmias were associated with progressive cardiomyopathy and fibrosis in Jup mutant hearts. PMID: 21880664
    14. Nuclear plakoglobin is essential for differentiation of cardiac progenitor cells to adipocytes in arrhythmogenic right ventricular cardiomyopathy. PMID: 22021931
    15. Plakoglobin (JUP) knockout mice exhibited progressive loss of cardiac myocytes, extensive inflammatory infiltration, fibrous tissue replacement, and cardiac dysfunction similar to those of arrhythmogenic right ventricular cardiomyopathy patients. PMID: 21245375
    16. Data propose a novel role for PG in regulating cell motility through distinct ECM-Src and RhoGTPase-dependent pathways, influenced in part by PG-dependent regulation of FN mRNA stability. PMID: 20876660
    17. PG strengthens adhesion and suppresses motility in mouse keratinocytes, through both intercellular adhesion-dependent and -independent mechanisms PMID: 15805189
    18. plakoglobin-dependent adhesion includes desmosome-specific functions in assembly and regulation by epidermal growth factor receptor PMID: 16183992
    19. These data confirmed that beta-catenin or plakoglobin is required for alpha-catenin to form complex with E-cadherin,It is also reqd for the stable expression and cell surface localization of E-cadherin. PMID: 16357441
    20. O-glycosylation functions to regulate the post-translational stability of plakoglobin and keratinocyte cell-cell adhesion PMID: 16510446
    21. Data show suppression of desmoplakin expression leads to nuclear localization of plakoglobin and a 2-fold reduction in canonical Wnt/beta-catenin signaling through Tcf/Lef1 transcription factors. PMID: 16823493
    22. Plakoglobin is a potent modulator of epithelial homeostasis via its role as a key suppressor of c-Myc in the skin. PMID: 16871158
    23. The results suggest that upregulation of gamma-catenin can compensate for the loss of beta-catenin in cardiomyocytes to maintain normal cardiac structure and function. PMID: 16936006
    24. Heterozygous plakoglobin deficiency provokes arrhythmogenic right ventricular cardiomyopathy. PMID: 17030684
    25. Plakoglobin may potentiate death in cells damaged by apoptotic stimuli, perhaps limiting the potential for the propagation of mutations and cellular transformation. PMID: 17110936
    26. PG is a principle effector of the PVIgG-induced signals downstream of c-Myc that disrupt the desmosomal plaque at the plasma membrane PMID: 17518986
    27. results exclude an essential role for canonical Wnt signaling (as mediated by beta- and/or gamma-catenin) during hematopoiesis and lymphopoiesis PMID: 17855627
    28. hematopoietic cells can transduce canonical Wnt signals in the combined absence of beta- and gamma-catenin PMID: 17906078
    29. plakoglobin is insufficient for LEF/TCF-dependent transcriptional activation by Wnt-3a in F9 cells PMID: 17984222
    30. Plakoglobin (PG) is a major component of the intracellular plaque that serves to connect transmembrane elements to the cytoskeleton. PMID: 18496566
    31. Data show that leukocytes interacting with endothelial cells rapidly dissociate VE-PTP from VE-cadherin, weakening endothelial cell contacts via a mechanism that requires plakoglobin but not beta-catenin. PMID: 19015309

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  • 亚细胞定位：
    Cell junction, adherens junction. Cell junction, desmosome. Cytoplasm, cytoskeleton. Membrane; Peripheral membrane protein. Note=Cytoplasmic in a soluble and membrane-associated form.
  • 蛋白家族：
    Beta-catenin family
  • 数据库链接：

    KEGG: mmu:16480

    STRING: 10090.ENSMUSP00000001592

    UniGene: Mm.299774